Tissue-engineered trachea: A review

Law, Jia Xian; Liau, Ling Ling; Aminuddin, B S; Ruszymah, B H I

doi:10.1016/j.ijporl.2016.10.012

Cited by 94 publications

(75 citation statements)

References 92 publications

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“…Due to these appropriate characteristics, there is a significant interest for using tissue decellularization to provide ECM-based scaffolds (Choi et al 2012; Costa et al 2007; Hellström et al 2014; Kiyotake et al 2016; Law et al 2016). …”

Section: Introductionmentioning

confidence: 99%

A silk fibroin/decellularized extract of Wharton’s jelly hydrogel intended for cartilage tissue engineering

et al. 2019

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A hybrid hydrogel was obtained from decellularized extract from Wharton’s jelly (DEWJ) and silk fibroin (SF) and characterized for cartilage tissue engineering. Wharton’s jelly was used due to its similarity with articular cartilage in extracellular matrix composition. Also, silk fibroin has good mechanical properties which make this construct appropriate for cartilage repair. Decellularization of Wharton’s jelly was verified by DAPI staining, DNA quantification, and PCR analysis. Then, the biochemical composition of DEWJ was determined by ELISA kits for total proteins, collagens, sulfated glycosaminoglycans (sGAG), and transforming growth factor β1 (TGF-β1). After fabricating pure SF and SF/DEWJ hybrid hydrogels, their physical and mechanical properties were characterized by FESEM, Fourier-transform infrared spectroscopy (FTIR) and rheological assays (amplitude and frequency sweeps). Furthermore, cell viability and proliferation were assessed by MTT assay. The results have shown that DEWJ in hybrid hydrogels enhances mechanical properties of the construct relative to pure SF hydrogels. Also, this extract at its 40% concentration in culture media and 20% or 40% concentrations in SF/DEWJ hybrid hydrogels significantly increases population of the cells compared to control and pure SF hydrogel after 7 days. In conclusion, this study proposes the potential of SF/DEWJ hybrid hydrogels for cartilage tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

A silk fibroin/decellularized extract of Wharton’s jelly hydrogel intended for cartilage tissue engineering

et al. 2019

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“…Three major shortcomings are described for current tissue engineered airway substitutes: a lack of mechanical stability, a missing long‐term stable sealed connection to the patient's trachea, and an insufficient blood supply with nutrition of the engineered tissue in vivo (Law et al, ). In addition, a functional ciliated epithelium is of high significance, as it prevents mucus plugging by mucociliary clearance and thus decreases the risk for infections (Hamilton et al, ; Zhang et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…airway tissue engineering, hydrogels, respiratory epithelium, tri-culture, vascularization 1 | INTRODUCTION Even though the trachea seems to be a simple tube to conduct air into the lungs, it is a complex organ with manifold functions. To allow for head movements and swallowing, it has to be flexible; to prevent collapse while inspiration or coughing, it has to be rigid (Law, Liau, Aminuddin, & Ruszymah, 2016). The airway mucosa is responsible to protect the lungs by warming, humidifying, and filtering the incoming air (Hamilton, Bullock, Macneil, Janes, & Birchall, 2014).…”

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confidence: 99%

“…As airway transplantation still has major shortcomings such as restenosis, immunological rejection, lack of blood supply, or infections, longer defects can only be treated palliatively (Delaere, 2012;Etienne et al, 2018) Thus, the development of an adequate tissue-engineered tracheal substitute is of high clinical interest. Three major shortcomings are described for current tissue engineered airway substitutes: a lack of mechanical stability, a missing long-term stable sealed connection to the patient's trachea, and an insufficient blood supply with nutrition of the engineered tissue in vivo (Law et al, 2016). In addition, a functional ciliated epithelium is of high significance, as it prevents mucus plugging by mucociliary clearance and thus decreases the risk for infections (Hamilton et al, 2014;Zhang et al, 2015).…”

mentioning

confidence: 99%

“…To the authors' knowledge, no studies have reported this so far. In respiratory tissue engineering, researchers have focused on functional regeneration of cartilage or functional respiratory epithelium (Kaye, Goldstein, Grande, Zeltsman, & Smith, 2019;Law et al, 2016). Mostly, only co-cultures of epithelial cells with fibroblasts or stem cells were used (Albers, Thiebes, Gessenich, Jockenhoevel, & Cornelissen, 2015;Law et al, 2016).…”

mentioning

confidence: 99%

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Combination of vascularization and cilia formation for three‐dimensional airway tissue engineering

Kreimendahl

Ossenbrink

Köpf

et al. 2019

J Biomedical Materials Res

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Tissue engineering is a promising approach to treat massive airway dysfunctions such as tracheomalacia or tumors. Currently, there is no adequate solution for patients requiring the resection of more than half of the length of their trachea. In this study, the best conditions for combination of three different cell types from the respiratory airway system were investigated to develop a functional ciliated and pre‐vascularized mucosal substitute in vitro. Primary human fibroblasts were combined with respiratory epithelial cells and endothelial cells. As scaffolds, fibrin gel and agarose‐type I collagen blends were used and cultured with different medium compositions to optimize both vascularization and differentiation of the respiratory epithelium. A mixture of endothelial growth medium and epithelial differentiation medium was shown to optimize both vascularization and epithelial growth and differentiation. After 28 days of co‐culture, significantly increased formation of capillary‐like structures was observed in fibrin gels with more than three times higher structure volumes compared to agarose–collagen gels. After 35 days, epithelial differentiation into a pseudostratified epithelium with typical marker expression was improved on fibrin gels. While cilia formation was shown on both scaffolds, a higher number of ciliated cells and longer cilia were observed on fibrin gels. The data elucidate the important interplay of co‐culture parameters and their impact on vascularization as well as epithelium development and provide a basis for development of functional three‐dimensional airway constructs.

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Biomimetic Trachea Regeneration Using a Modular Ring Strategy Based on Poly(Sebacoyl Diglyceride)/Polycaprolactone for Segmental Trachea Defect Repair

Guo

et al. 2020

Adv Funct Materials

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The lack of a biomimetic tracheal substitute featured with cartilage ring/ vascularized connective tissue alternate structure (CVCAS) has significantly retarded a clinical breakthrough of tissue-engineered trachea in repairing extensive tracheal lesions. Herein, a novel modular strategy based on cartilage rings and biomaterial rings is used to generate a biomimetic trachea with CVCAS. Poly(sebacoyl diglyceride) and polycaprolactone (PCL) (PSeD/PCL) are blended to fabricate a porous scaffold with satisfactory mechanical properties, biodegradability, and biocompatibility. A cartilage ring is engineered in vitro using ring-shaped PSeD/PCL scaffold loaded with chondrocytes and cartilage tube is generated in vivo through the fusion of multiple modular cartilage rings. More importantly, biomimetic tracheas with CVCAS are successfully fabricated in vivo through alternately stacked cartilage rings and PSeD/PCL rings on a silicon tube. Notably, the engineered biomimetic trachea exhibits not only a bionic structure but also superior mechanical properties compared with the native rabbit trachea. Finally, segmental tracheal defects in rabbits are repaired by transplanting the biomimetic trachea with pedicled muscular flaps. The biomimetic trachea establishes stable blood supply and provided sufficient mechanical strength and biological function similar to those of native trachea. This work represents the first successful regeneration of biomimetic trachea with CVCAS and a promising approach for clinical application.

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Tissue-engineered trachea: A review

Cited by 94 publications

References 92 publications

A silk fibroin/decellularized extract of Wharton’s jelly hydrogel intended for cartilage tissue engineering

A silk fibroin/decellularized extract of Wharton’s jelly hydrogel intended for cartilage tissue engineering

Combination of vascularization and cilia formation for three‐dimensional airway tissue engineering

Biomimetic Trachea Regeneration Using a Modular Ring Strategy Based on Poly(Sebacoyl Diglyceride)/Polycaprolactone for Segmental Trachea Defect Repair

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