Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair

Gao, Manchen; Zhang, Heng‐Yi; Dong, Wei; Bai, Jie; Gao, Botao; Xia, Dekai; Feng, Bei; Chen, Maolin; He, Xiaomin; Yin, Meng; Xu, Zhiwei; Witman, Nevin; Fu, Wei; Zheng, Jie

doi:10.1038/s41598-017-05518-3

Cited by 106 publications

(110 citation statements)

References 29 publications

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“…All scaffolds were produced in Beijing Advanced Medical Technologies Ltd., Inc (Beijing, China). Rapid stent fabrication system was used as a preparation system of the scaffold according to the published methods (Gao et al, ). The scaffold fabrication system is a 3D‐printing system equipped with an additional rotation mandrel driven by a servomotor.…”

Section: Methodsmentioning

confidence: 99%

“…Goat auricular chondrocytes were isolated and expanded as previously described (Gao et al, ). Under sterile conditions, PCL tracheal scaffolds were inoculated in a trachea scaffold custom glass dish (patent), together with a chondrocyte suspension gel made by 5‐ml cartilage cell suspension with 1.2 * 10 8 chondrocytes and type I collagen (Hangzhou Sunyoung biotech Co., Ltd., Hangzhou, China).…”

Section: Methodsmentioning

confidence: 99%

“…Blank scaffolds and tissue‐engineered scaffolds were analysed by scanning electron microscopy at Shanghai Jiao Tong University Bio‐X Center after 4 weeks of in vitro culture to observe cell adhesion on scaffolds as previously described (Gao et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Post‐operative survival times were recorded along with a detailed autopsy at time of death to monitor the cause of death. The tracheal implants of the deceased goats were immediately harvested and prepared for analysis (Gao et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Still, many researchers are limited to simple subcutaneous transplantation or flaky tracheal repair, which undermines assessment in regard to clinical application, as clinicians in general are in need of whole tracheas for transplantation. In our previous study, we demonstrated the feasibility of repairing whole‐segment tracheal defects with 3D‐printed TETs in a rabbit model (Gao et al, ). On the basis of this study, we 3D printed a scaffold of the whole segment of goat trachea and constructed a TET with autologous auricular cartilage cells.…”

Section: Introductionmentioning

confidence: 99%

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Tissue‐engineered trachea from a 3D‐printed scaffold enhances whole‐segment tracheal repair in a goat model

Xia

Jin

Wang

et al. 2019

J Tissue Eng Regen Med

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Traditional treatment therapies for tracheal stenosis often cause severe postoperative complications. To solve the current difficulties, novel and more suitable long-term treatments are needed. A whole-segment tissue-engineered trachea (TET) representing the native goat trachea was 3D printed using a poly(caprolactone) (PCL) scaffold engineered with autologous auricular cartilage cells. The TET underwent mechanical analysis followed by in vivo implantations in order to evaluate the clinical feasibility and potential. The 3D-printed scaffolds were successfully cellularized, as observed by scanning electron microscopy. Mechanical force compression studies revealed that both PCL scaffolds and TETs have a more robust compressive strength than does the native trachea. In vivo implantation of TETs in the experimental group resulted in significantly higher mean post-operative survival times, 65.00 ± 24.01 days (n = 5), when compared with the control group, which received autologous trachea grafts, 17.60 ± 3.51 days (n = 5). Although tracheal narrowing was confirmed by bronchoscopy and computed tomography examination in the experimental group, tissue necrosis was only observed in the control group.Furthermore, an encouraging epithelial-like tissue formation was observed in the TETs after transplantation. This large animal study provides potential preclinical evidence around the employment of an orthotopic transplantation of a whole 3D-printed TET.One sentence summary: In this study, we employed 3D-printing technology fused with in vitro culturing of chondrocytes in order to design and construct a whole-segment tissue-engineered trachea (TET) for regeneration studies in a large animal model. * These authors contributed equally to this work.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Tissue‐engineered trachea from a 3D‐printed scaffold enhances whole‐segment tracheal repair in a goat model

Xia

Jin

Wang

et al. 2019

J Tissue Eng Regen Med

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Bioengineering and Clinical Translation of Human Lung and its Components

et al. 2023

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Clinical lung transplantation has rapidly established itself as the gold standard of treatment for end‐stage lung diseases in a restricted group of patients since the first successful lung transplant occurred. Although significant progress has been made in lung transplantation, there are still numerous obstacles on the path to clinical success. The development of bioartificial lung grafts using patient‐derived cells may serve as an alternative treatment modality; however, challenges include developing appropriate scaffold materials, advanced culture strategies for lung‐specific multiple cell populations, and fully matured constructs to ensure increased transplant lifetime following implantation. This review highlights the development of tissue‐engineered tracheal and lung equivalents over the past two decades, key problems in lung transplantation in a clinical environment, the advancements made in scaffolds, bioprinting technologies, bioreactors, organoids, and organ‐on‐a‐chip technologies. The review aims to fill the lacuna in existing literature toward a holistic bioartificial lung tissue, including trachea, capillaries, airways, bifurcating bronchioles, lung disease models, and their clinical translation. Herein, the efforts are on bridging the application of lung tissue engineering methods in a clinical environment as it is thought that tissue engineering holds enormous promise for overcoming the challenges associated with the clinical translation of bioengineered human lung and its components.

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Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair

Duan

et al. 2020

Adv Funct Materials

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Wharton's jelly (WJ) is considered a potential scaffold in tissue‐engineered trachea for its similar composition and function to cartilage tissue. However, the feasibility of using WJ to construct engineered neocartilage tissue has not been reported, let alone tubular tracheal cartilage regeneration and segmental tracheal lesion repair. Here, electrospun nanofibrous membranes composed of three different decellularized WJ matrix (DWJM)/poly(ε‐caprolactone) (PCL) ratios (8:2, 5:5, and 2:8) are fabricated. The results demonstrate improved degradation speed, absorption, and cell adhesion capacity but weakened mechanical properties with increased DWJM content, but satisfactory homogeneous cartilage regeneration is only achieved in the DWJM/PCL (8:2) group after 12 weeks in vivo culture. Furthermore, homogeneous, 3D, tubular, trachea‐shaped cartilage is constructed with a controllable lumen diameter and wall thickness based on the 2D nanofibrous membrane using a modified sandwich model, in which the chondrocyte‐membrane construct is rolled around a silicon tube. Most importantly, by combining the above schemes with previously established vascularization and epithelialization techniques, chondrification, vascularization, and epithelialization are achieved simultaneously thus realizing long‐term (6 months) circumferential tracheal lesion repair in a rabbit model with a biological structure and function similar to that of native trachea, representing a promising approach for the clinical application of tracheal tissue engineering.

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Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair

Cited by 106 publications

References 29 publications

Tissue‐engineered trachea from a 3D‐printed scaffold enhances whole‐segment tracheal repair in a goat model

Tissue‐engineered trachea from a 3D‐printed scaffold enhances whole‐segment tracheal repair in a goat model

Bioengineering and Clinical Translation of Human Lung and its Components

Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair

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