Tissue Engineering a Complete Vaginal Replacement From a Small Biopsy of Autologous Tissue

Philippo, Roger E. De; Bishop, Colin E.; Filho, Luiz Freitas; Yoo, James J.; Atala, Anthony

doi:10.1097/tp.0b013e31817f1686

Cited by 96 publications

(55 citation statements)

References 13 publications

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“…The cell-seeded matrices were able to regenerate the typical tri-layer vaginal tissue structure with adequate functional parameters long-term ( Figure 3). 9 Similar findings were present when engineering other tissues, such as blood vessels, heart valves, salivary glands, penile tissue, and nerves. [10][11][12][13][14] These studies to date show that scars do not necessarily form for small or superficial wounds or injuries but may be present for deep or larger wounds, often over 3 mm in diameter.…”

Section: Principles Of Wound Healing and Tissue Regenerationsupporting

confidence: 60%

Wound Healing Versus Regeneration: Role of the Tissue Environment in Regenerative Medicine

Atala¹,

Irvine²,

Moses³

et al. 2010

MRS Bull.

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One of the major challenges in the field of regenerative medicine is how to optimize tissue regeneration in the body by therapeutically manipulating its natural ability to form scar at the time of injury or disease. It is often the balance between tissue regeneration, a process that is activated at the onset of disease, and scar formation, which develops as a result of the disease process that determines the ability of the tissue or organ to be functional. Using biomaterials as scaffolds often can provide a “bridge” for normal tissue edges to regenerate over small distances, usually up to 1 cm. Larger tissue defect gaps typically require both scaffolds and cells for normal tissue regeneration to occur without scar formation. Various strategies can help to modulate the scar response and can potentially enhance tissue regeneration. Understanding the mechanistic basis of such multivariate interactions as the scar microenvironment, the immune system, extracellular matrix, and inflammatory cytokines may enable the design of tissue engineering and wound healing strategies that directly modulate the healing response in a manner favorable to regeneration.

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Section: Principles Of Wound Healing and Tissue Regenerationsupporting

confidence: 60%

Wound Healing Versus Regeneration: Role of the Tissue Environment in Regenerative Medicine

Atala¹,

Irvine²,

Moses³

et al. 2010

MRS Bull.

Self Cite

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“…Other tissue sources, including the skin, trachea, and urethral tissue (Raya-Rivera et al, 2011) are also considered (Badylak et al, 2012). Animal studies have indicated that native vaginal tissue is preferable for reconstruction (De Filippo et al, 2008). For clinical practice, unfortunately, native vaginal tissue is limited or unavailable at all in some cases.…”

Section: Introductionmentioning

confidence: 99%

Bone marrow mesenchymal stem cells could acquire the phenotypes of epithelial cells and accelerate vaginal reconstruction combined with small intestinal submucosa

Liu

Zhang

et al. 2015

Cell Biology International

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Grafting material for vaginal reconstruction commonly includes the bowel, peritoneum, skin, and amniotic membrane. Bone marrow mesenchymal stem cells (MSCs) have the potential of multilineage differentiation into a variety of cells and have been widely explored in tissue engineering. In the current study, we examined whether MSCs could be differentiated to vaginal epithelial cells (VECs) upon co-culturing with VECs. We also examined whether Wnt/β-catenin signaling pathway is implicated in such differentiation. Co-culture of MSCs with VECs using a transwell insert system (with no direct contact) induced the expression of VECs marker AE1/AE3 in MSCs. MSCs combined with small intestinal submucosa (SIS) scaffold were implanted in place of the native vagina in rats to observe the implications for vaginal reconstruction in vivo. Anatomic repair of neovagina was assessed by histological staining for H/E and Masson's Trichrome. GSK-3β and β-catenin, main members of Wnt/β-catenin signaling pathway, in MSCs were increased upon co-culturing with VECs. Exposure of co-cultured MSCs to a Wnt/β-catenin signaling activator, lithium chloride (LiCl, 20 µM) increased phosphorylated GSK-3β and β-catenin and enhanced expression of AE1/AE3. In vivo-grafted cells displayed significant matrix infiltration and expressed epithelial markers in neovagina. These findings suggest that MSCs could acquire the phenotype of VECs when co-cultured with VECs, possibly via activation of Wnt/β-catenin signaling. MSCs provide an alternative cell source for potential use in vaginal tissue engineering.

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“…De Fillipo et al reported the results of vaginal replacement in a rabbit model using a neovagina engineered from biopsy of autologous vaginal tissue. The presence of functional and histologically normal vaginal tissues represented the opportunity of a very attractive option for surgery reconstruction in patients with MRKH syndrome [24]. Pacini et al reported a case report of modified Abbé-McIndoe technique using autologous in vitro grown vaginal tissue in a patient with vaginal agenesis.…”

Section: Discussionmentioning

confidence: 99%

Role of estrogen receptor alpha on vaginal epithelialization of patients with Mayer–Rokitansky–Kuster–Hauser syndrome submitted to neovaginoplasty using oxidized regenerated cellulose

Kajikawa¹,

Bella

Focchi

et al. 2012

Int Urogynecol J

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Tissue Engineering a Complete Vaginal Replacement From a Small Biopsy of Autologous Tissue

Abstract: These data indicate that a tissue engineering approach to clinical vaginal reconstruction in women is now a realistic possibility.

Cited by 96 publications

References 13 publications

Wound Healing Versus Regeneration: Role of the Tissue Environment in Regenerative Medicine

Wound Healing Versus Regeneration: Role of the Tissue Environment in Regenerative Medicine

Bone marrow mesenchymal stem cells could acquire the phenotypes of epithelial cells and accelerate vaginal reconstruction combined with small intestinal submucosa

Role of estrogen receptor alpha on vaginal epithelialization of patients with Mayer–Rokitansky–Kuster–Hauser syndrome submitted to neovaginoplasty using oxidized regenerated cellulose

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