Tissue engineering: A new approach in cardiovascular surgery; Seeding of human fibroblasts followed by human endothelial cells on resorbable mesh1

Zünd, Gregor; Hoerstrup, Simon P.; Schoeberlein, Andreina; Lachat, Mario; Uhlschmid, G; Vogt, Paul; Turina, Marko

doi:10.1016/s1010-7940(97)00309-6

Cited by 80 publications

(40 citation statements)

References 12 publications

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“…PLA is a biocompatible and biodegradable synthetic polymeric material that has attracted great attention as a surgical repair material, e.g., for the development of synthetic vascular grafts (29)(30)(31) and medical sutures (32,33) and as a temporary scaffold for cell growth in tissue-engineering applications (14,15,(17)(18)(19). Generally, scaffolds fabricated from PLA, a hydrophobic polymer, have to be pretreated with ethanol to achieve cell spreading.…”

Section: Resultsmentioning

confidence: 99%

Self-assembling biomaterials: Liquid crystal phases of cholesteryl oligo(l-lactic acid) and their interactions with cells

Hwang

Iyer²,

Li³

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

136

103

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We report here on the synthesis and characterization of a series of self-assembling biomaterials with molecular features designed to interact with cells and scaffolds for tissue regeneration. The molecules of these materials contain cholesteryl moieties, which have universal affinity for cell membranes, and short chains of lactic acid, a common component of biodegradable tissue engineering matrices. The materials were synthesized in good yields with low polydispersities in the range of 1.05-1.15, and their characterization was carried out by small-angle x-ray diffraction, transmission electron microscopy, electron diffraction, differential scanning calorimetry, and atomic force microscopy. These molecular materials form layered structures that can be described as smectic phases and can also order into singlecrystal stacks with an orthorhombic unit cell. Their layer spacings range from 58 to 99 Å, corresponding to bilayers of oligomers with an average of 10 and 37 lactic acid residues, respectively. The selforganized layered structures were found to promote improved fibroblast adhesion and spreading, although the specific mechanism for this observed response remains unknown. The ability of self-assembling materials to present ordered and periodic bulk structures to cells could be a useful strategy in tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Self-assembling biomaterials: Liquid crystal phases of cholesteryl oligo(l-lactic acid) and their interactions with cells

Hwang

Iyer²,

Li³

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

136

103

View full text Add to dashboard Cite

show abstract

“…Out of these vessels, two cell types can be isolated: Endothelial cells (ECs) with antithrombogenic properties and myofibroblasts capable of ECM development [16][17][18]. After preliminary studies, mainly in sheep [16][17][18][19][20][21], the potential of human vascular-derived cells was evaluated by seeding on biodegradable scaffolds and revealed excellent tissue formation [15,22,23]. A promising alternative cell source for regenerative medicine is bone marrow-derived stem cells (BMSCs) [3,24,25].…”

Section: Strategies In Tissue Engineering: In Vivo or Ex Vivo?mentioning

confidence: 99%

“…Differentiated ECs have been isolated from vascular sources exhibiting promising results in heart valve tissue engineering [16,19,20,22]. Furthermore, endothelial Another interesting cell source is the umbilical cord, containing several cell types that can be used for heart valve tissue engineering: (1) umbilical cord vein-derived and artery-derived cells, (2) Wharton's Jelly-derived MSCs, and (3) umbilical cord blood-derived EPCs.…”

Section: Strategies In Tissue Engineering: In Vivo or Ex Vivo?mentioning

confidence: 99%

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Tissue engineering on matrix: future of autologous tissue replacement

et al. 2011

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“…Seeding cells onto three-dimensional scaffolds is sequential -myofibroblasts are seeded first, followed by endothelial cells [56]. Due to their better self-renewal capacity, broader plasticity, and immunomodullatory properties comparable to adult fibroblasts, tissue-engineered constructs based on perinatal stem cells in general (and particularly WJ-MSCs) could be an attractive alternative to classical cardiovascular substitutes.…”

Section: Application Of Wj-mscs In Cardiovascu-lar Tissue Engineeringmentioning

confidence: 99%

Mesenchymal Stem Cells Derived from Wharton's Jelly and their Potential for Cardio-Vascular Tissue Engineering

Semenov¹

2011

TOTERMJ

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Abstract:Experimental results accumulated during last decade suggest that human perinatal tissues such as placenta, fetal membranes, and umbilical cord, as well as perinatal fluids such as, amniotic fluid and umbilical cord blood, harbour different amounts of multipotent precursor cells, called extra-embryonic mesenchymal stem cells (EE-MSCs). Perinatal EE-MSCs represent an intermediate cell type between pluripotent embryonic stem cells (ESCs) and multipotent MSCs derived from variety of postnatal human tissues, such as bone marrow, fat, dental pulp, etc. Multipotent mesenchymal cells obtained from connective Wharton s Jelly tissue of umbilical cord (WJ-MSCs) currently emerged as particularly interesting type of perinatal EE-MSCs, related for therapeutical applications and cryobanking. These cells are easily assessable for isolation, possess fetal karyotype, and hold very active growth potential. Due to their unique developmental position WJ-MSCs exhibit specific phenotype which combines some markers expressed by postnatal bone marrowderived MSCs, such as CD73, CD90, and CD105 with some markers typically expressed by ESCs, such as Oct-3/4, Sox-2, and Nanog. In terms of plasticity WJ-MSCs demonstrate the potential for differentiation towards mesodermal and ectodermal lineages in vitro and in vivo. Additionally, extraembryonic tissues are normally discarded after birth and the isolation of WJ-MSCs is free of ethical concerns. In this work we aim to review previously obtained experimental results, discuss different aspects concerning plasticity and immunomodulatory characteristics of WJ-MSCs, and evaluate the potential of these cells for biomedical and clinical applications.

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Tissue engineering: A new approach in cardiovascular surgery; Seeding of human fibroblasts followed by human endothelial cells on resorbable mesh1

Abstract: These results are a first step to demonstrate that seeding of human fibroblasts and endothelial cells on PGA-mesh might be a feasible model to construct human tissues such as vessels or cardiac valves.

Cited by 80 publications

References 12 publications

Self-assembling biomaterials: Liquid crystal phases of cholesteryl oligo(l-lactic acid) and their interactions with cells

Self-assembling biomaterials: Liquid crystal phases of cholesteryl oligo(l-lactic acid) and their interactions with cells

Tissue engineering on matrix: future of autologous tissue replacement

Mesenchymal Stem Cells Derived from Wharton's Jelly and their Potential for Cardio-Vascular Tissue Engineering

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