Tissue engineering and autologous transplant formation: practical approaches with resorbable biomaterials and new cell culture techniques

Sittinger, Michael; Bujía, J; Rotter, Nicole; Reitzel, D.; Minuth, Will W.; Burmester, Gerd R.

doi:10.1016/0142-9612(96)85561-x

Cited by 201 publications

(109 citation statements)

References 28 publications

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“…In order to disassemble the complex pathogenetic process of RA, we established a second model system that has the potential to separately study isolated cell populations. To achieve this goal, an "artificial cartilage" was first established: a 3-D culture of chondrocytes synthesizing their own cartilage-specific matrix (27,37,41). The advantage of the 3-D gels used is their flexibility and malleability, allowing us to rearrange the cell architecture in a 3-D orientation.…”

Section: Discussionmentioning

confidence: 99%

Development of in vitro model systems for destructive joint diseases. Novel strategies for establishing inflammatory pannus

Schultz

Keyszer

Zacher

et al. 1997

Arthritis & Rheumatism

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Objective. To establish a novel 3-dimensional (3-D) in vitro model for the investigation of destructive processes in rheumatoid arthritis (RA).Methods. Two distinct culture systems were developed, consisting of RA synovial membrane and articular cartilage explants or interactive RA synovial cell/ chondrocyte cultures embedded in 3-D fibrin matrices. The expression of proteolytic enzymes, chondrocyte matrix architecture, and matrix degradation parameters was analyzed by immunohistochemistry.Results. Of 28 RA explant cultures, 16 displayed an invasion of synovial tissue into the cartilage explants, compared with 1 of 8 osteoarthritis explants. The expression of collagenase and vascular cell adhesion molecule l could be demonstrated at the cartilage-pannus junction. Of 20 interactive cell cultures, 18 revealed invasive behavior and remained vital for extended periods of time.Conclusion. The models presented allow us to study distinct aspects of destructive joint diseases under in vitro conditions that resemble human pathology. Moreover, our model is able to supplement animal experiments in basic research and drug testing.In rheumatoid arthritis (RA), the physiologic balance between matrix deposition and degradation is Supported by the Deutsche Forschungsgemeinschaft (grant Bu 44513-1 and 3-2) and the Hildegard Doerenkamp-Gerhard Zbinden Foundation.

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Section: Discussionmentioning

confidence: 99%

Development of in vitro model systems for destructive joint diseases. Novel strategies for establishing inflammatory pannus

Schultz

Keyszer

Zacher

et al. 1997

Arthritis & Rheumatism

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“…[9][10][11] A local slaughterhouse supplied freshly slaughtered adult bovine forelimbs. Pieces of articular cartilage of the humeral head were surgically removed under sterile conditions.…”

Section: Methodsmentioning

confidence: 99%

“…8 Alternatively, engi-neered cartilage tissue, which may be cultured for orthopedic purposes from nonarticular locations, provides the potential of ample amounts to fill defects and span large distances. [9][10][11] As an alternative to the replacement of defect tissue by cartilage or cartilage-like tissue, such voids may be filled with a substance that matures in vivo and potentially may bear full weight in the long term. 12 If post-traumatic lesions are treated early, restoration of the initial joint geometry and integrity may be achieved.…”

Section: Introductionmentioning

confidence: 99%

Mechanical quality of tissue engineered cartilage: Results after 6 and 12 weeks in vivo

Duda

Haisch

Endres

et al. 2000

J. Biomed. Mater. Res.

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Traumatic events are a primary cause for local lesions of articular cartilage. If treated early, restoration of the initial joint geometry and integrity may be achieved. In large defects, sufficient material is not available to bridge the affected area. Heterologeous transplantation is not well accepted due to the risk of infection and immune response. Alternatives are cartilage-like structures, which may be cultured in vitro and transplanted into the defect site. Critical to the success of these new tissues are their mechanical properties. Goals of this study were to generate a hyaline-like cartilage structure, to evaluate its performance in vivo and to verify that its cellular and material properties meet those of native cartilage. Hyalinelike cartilage specimens were generated in vitro and implanted in the backs of nude mice. Specimens were explanted after 6 and 12 weeks, mechanically tested using an indentation test and histologically examined. In mechanical testing, stiffness and failure load significantly increased between weeks 6 and 12. At 12 weeks, mechanical properties of the hyaline-like cartilage were comparable to those of native nasal septal cartilage. Compared to native articular cartilage, the engineered tissue achieved up to 30 -50% in strength and mechanical stiffness. In histological examination, specimens showed neocartilage formation. The mechanical testing procedure proved to be sufficiently sensitive to identify differences in properties between cartilage specimens of different origin and at different stages of healing. As an adjunct to histological analysis, mechanical testing may be a valuable tool for judging the utility of engineered cartilage prior to a broad clinical usage.

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“…(Sittinger et al, 1996;Vacanti & Langer, 1999;Khademhosseini et al, 2009). Some degradable polymers, ceramics, or a combination of both can provide desirable mechanical and osteoconductive properties as basic scaffold material for bone replacement (Zippel et al, 2010b).…”

Section: Stem Cells For Bone Regenerationmentioning

confidence: 99%

Oral Tissues as Source for Bone Regeneration in Dental Implantology

Khan¹,

Kleinfeld²,

Winter³

et al. 2012

Tissue Regeneration - From Basic Biology to Clinical Application

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Tissue engineering and autologous transplant formation: practical approaches with resorbable biomaterials and new cell culture techniques

Cited by 201 publications

References 28 publications

Development of in vitro model systems for destructive joint diseases. Novel strategies for establishing inflammatory pannus

Development of in vitro model systems for destructive joint diseases. Novel strategies for establishing inflammatory pannus

Mechanical quality of tissue engineered cartilage: Results after 6 and 12 weeks in vivo

Oral Tissues as Source for Bone Regeneration in Dental Implantology

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