Tissue Engineering and Regenerative Strategies to Replicate Biocomplexity of Vascular Elastic Matrix Assembly

Bashur, Chris A.; Venkataraman, Lavanya; Ramamurthi, Anand

doi:10.1089/ten.teb.2011.0521

Cited by 48 publications

(42 citation statements)

References 139 publications

(149 reference statements)

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“…This hypothesis is supported by the earlier upregulation of fibulin 5 mRNA at 4 weeks after implantation for the blend versus PCL constructs. Fibulin 5 is a critical component of microfibrillar scaffolds that have been shown to bind elastin [2]. Our results suggest that the machinery exists for elastic fiber assembly within the collagen – PCL blend conduits.…”

Section: Resultsmentioning

confidence: 73%

“…While tissue engineered grafts have been investigated as potential alternatives, several challenges have yet to be overcome. One challenge is the poor generation of mature elastic matrix by most adult cells [2, 3]. Elastic matrix in healthy arteries enables both vessel recoil after stretch and regulation of smooth muscle cell (SMC) phenotype [4].…”

Section: Introductionmentioning

confidence: 99%

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Composition of intraperitoneally implanted electrospun conduits modulates cellular elastic matrix generation

Bashur

Ramamurthi

2014

Acta Biomaterialia

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Improving elastic matrix generation is critical to developing functional tissue engineered vascular grafts. Therefore, this study pursued a strategy to grow autologous tissue in vivo by recruiting potentially more elastogenic cells to conduits implanted within the peritoneal cavity. The goal was to determine the impacts of electrospun conduit composition and hyaluronan oligomer (HA-o) modification on the recruitment of peritoneal cells, and their phenotype and ability to synthesize elastic matrix. These responses were assessed as a function of conduit intraperitoneal implantation time. This study showed that the blending of collagen with poly(ε- caprolactone)(PCL) promotes a faster wound healing response, as assessed by trends in expression of macrophage and smooth muscle cell (SMC) contractile markers and in matrix deposition, compared to the more chronic response for PCL alone. This result, along with the increase in elastic matrix production, demonstrates the benefits of incorporating a little as 25% w/w collagen into the conduit. In addition, PCR analysis demonstrated the challenges in differentiating between a myofibroblast and a SMC using traditional phenotypic markers. Finally, the impact of the tethered HA-o is limited within the inflammatory environment, unlike the significant response found previously in vitro. In conclusion, these results demonstrate the importance of both careful control of implanted scaffold composition and the development of appropriate delivery methods for HA-o.

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Section: Resultsmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Composition of intraperitoneally implanted electrospun conduits modulates cellular elastic matrix generation

Bashur

Ramamurthi

2014

Acta Biomaterialia

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“…Via biomechanical transduction, elastin plays an equally critical role in regulating cell signaling pathways ( e.g. , those of smooth muscle cells and luminal endothelial cells in the arterial wall) involved in morphogenesis, inflammation and injury response [451,452]. Moreover, it has been demonstrated that elastin is an important autocrine factor that ensures vascular homeostasis via a combination of biologic signaling and biomechanical support.…”

Section: Human Tissue Ecm-based Biomaterialsmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

959

513

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Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

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“…The authors concluded that, for vascular graft applications, the tensile strengths of the prTE scaffolds should be further increased by reinforcement or co-spinning with either collagen or synthetic materials. Interestingly, Bashur et al (90) have reviewed many important issues related to elastin-based TEVGs. Starting from a description of the current challenges that exist in the field of elastic matrix engineering, specifically for vascular applications, they continue by introducing the specific targets and processes that impact elastogenesis, and conclude with a review of current progress toward achieving robust elastogenesis in TEVGs aimed at clinical use.…”

Section: Elastin Into Scaffolds: the Keystone Of Tevgsmentioning

confidence: 99%

Bioengineered Vascular Scaffolds: The State of the Art

et al. 2014

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To date, there is increasing clinical need for vascular substitutes due to accidents, malformations, and ischemic diseases. Over the years, many approaches have been developed to solve this problem, starting from autologous native vessels to artificial vascular grafts; unfortunately, none of these have provided the perfect vascular substitute. All have been burdened by various complications, including infection, thrombogenicity, calcification, foreign body reaction, lack of growth potential, late stenosis and occlusion from intimal hyperplasia, and pseudoaneurysm formation. In the last few years, vascular tissue engineering has emerged as one of the most promising approaches for producing mechanically competent vascular substitutes. Nanotechnologies have contributed their part, allowing extraordinarily biostable and biocompatible materials to be developed. Specifically, the use of electrospinning to manufacture conduits able to guarantee a stable flow of biological fluids and guide the formation of a new vessel has revolutionized the concept of the vascular substitute. The electrospinning technique allows extracellular matrix (ECM) to be mimicked with high fidelity, reproducing its porosity and complexity, and providing an environment suitable for cell growth. In the future, a better knowledge of ECM and the manufacture of new materials will allow us to “create” functional biological vessels - the base required to develop organ substitutes and eventually solve the problem of organ failure.

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Tissue Engineering and Regenerative Strategies to Replicate Biocomplexity of Vascular Elastic Matrix Assembly

Cited by 48 publications

References 139 publications

Composition of intraperitoneally implanted electrospun conduits modulates cellular elastic matrix generation

Composition of intraperitoneally implanted electrospun conduits modulates cellular elastic matrix generation

Advancing biomaterials of human origin for tissue engineering

Bioengineered Vascular Scaffolds: The State of the Art

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