Tissue-Engineering Approaches to Restore Kidney Function

Katari, Ravi; Edgar, Lauren; Wong, Theresa; Boey, Angela; Mancone, Sarah; Igel, Daniel; Callese, Tyler E.; Voigt, Marcia; Tamburrini, Riccardo; Zambon, João Paulo; Perin, Laura; Orlando, Giuseppe

doi:10.1007/s11892-015-0643-0

Cited by 10 publications

(6 citation statements)

References 97 publications

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“…In addition to the problem of recellularization, there are other important3940 hurdles in the field related to the source of renal cells and the need to obtain, inside the kidney, differentiation of infused cells into numerous phenotypes, as found in the native organ. The general concept is that, using embryonic stem cells, differentiation can be induced through the contact of seeded cells with ECM proteins along the nephron, as well as the 3D structure of the ECM membranes.…”

Section: Discussionmentioning

confidence: 99%

Experimental Evaluation of Kidney Regeneration by Organ Scaffold Recellularization

Remuzzi

Figliuzzi

Bonandrini

et al. 2017

Sci Rep

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The rising number of patients needing renal replacement therapy, alongside the significant clinical and economic limitations of current therapies, creates an imperative need for new strategies to treat kidney diseases. Kidney bioengineering through the production of acellular scaffolds and recellularization with stem cells is one potential strategy. While protocols for obtaining organ scaffolds have been developed successfully, scaffold recellularization is more challenging. We evaluated the potential of in vivo and in vitro kidney scaffold recellularization procedures. Our results show that acellular scaffolds implanted in rats cannot be repopulated with host cells, and in vitro recellularization is necessary. However, we obtained very limited and inconsistent cell seeding when using different infusion protocols, regardless of injection site. We also obtained experimental and theoretical data indicating that uniform cell delivery into the kidney scaffolds cannot be obtained using these infusion protocols, due to the permeability of the extracellular matrix of the scaffold. Our results highlight the major physical barriers that limit in vitro recellularization of acellular kidney scaffolds and the obstacles that must be investigated to effectively advance this strategy for regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Experimental Evaluation of Kidney Regeneration by Organ Scaffold Recellularization

Remuzzi

Figliuzzi

Bonandrini

et al. 2017

Sci Rep

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“…Afterward, the clinical success of Macchiarini's engineered airway was questioned by the scientific community for several reasons [136], and he had to retract some of his papers. Nevertheless, the way toward the clinical translation of tissue-engineered constructs was opened and several tissues and organs are currently under development: heart and heart valves [137,138], liver [139], kidney [140], lung [141], bladder [142], bone [143] and, of course, skin [144], cartilage [145] and trachea [146], are some examples from the scientific literature.…”

Section: Some Words To Concludementioning

confidence: 99%

Biomaterials and Their Biomedical Applications: From Replacement to Regeneration

Todros¹,

Todesco²,

Bagno³

2021

Processes

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The history of biomaterials dates back to the mists of time: human beings had always used exogenous materials to facilitate wound healing and try to restore damaged tissues and organs. Nowadays, a wide variety of materials are commercially available and many others are under investigation to both maintain and restore bodily functions. Emerging clinical needs forced the development of new biomaterials, and lately discovered biomaterials allowed for the performing of new clinical applications. The definition of biomaterials as materials specifically conceived for biomedical uses was raised when it was acknowledged that they have to possess a fundamental feature: biocompatibility. At first, biocompatibility was mainly associated with biologically inert substances; around the 1970s, bioactivity was first discovered and the definition of biomaterials was consequently extended. At present, it also includes biologically derived materials and biological tissues. The present work aims at walking across the history of biomaterials, looking towards the scientific literature published on this matter. Finally, some current applications of biomaterials are briefly depicted and their future exploitation is hypothesized.

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“…Tissue engineering (TE) plays a pivotal role in revolutionizing and improving quality of life by restoring the biological functions of degenerated or injured tissues and organs [1,2]. The objective of TE, as an interdisciplinary science, is to integrate cells, engineered materials and biochemical factors to replace tissues and augment biological functions.…”

Section: Introductionmentioning

confidence: 99%

Sustained releasing sponge-like 3D scaffolds for bone tissue engineering applications

Chamundeswari¹,

Siang²,

Chuah³

et al. 2017

Biomed. Mater.

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Tissue engineering (TE) is envisaged to play a vital role in improving quality of life by restoring, maintaining or enhancing tissue and organ functions. TE scaffolds that are two-dimensional in structure suffer from undesirable issues, such as pore blockage, and do not closely mimic the native extra-cellular matrix in tissues. Significant efforts have therefore been channeled to fabricate three-dimensional (3D) scaffolds using various techniques, especially electrospinning. In this study, we propose a modified one-step electrospinning process to arrive at a 3D scaffold with highly interconnected pores. Using a blend of poly (L-lactide)/polycaprolactone/poly (ethylene oxide), this mechanically viable, sponge-like 3D scaffold exhibited sufficiently large pores and enabled cell penetration beyond 500 μm. Dexamethasone (Dex) was loaded into the fibers and a sustained drug release was achieved. Further, the potential of this Dex-loaded 3D scaffold was evaluated for upregulation of osteogenic genes with mesenchymal stem cells. The as-produced Dex-loaded 3D scaffold possesses a unique intertwined sub-micron fibrous morphology that can be tailored for use in bone tissue engineering and beyond.

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Tissue-Engineering Approaches to Restore Kidney Function

Cited by 10 publications

References 97 publications

Experimental Evaluation of Kidney Regeneration by Organ Scaffold Recellularization

Experimental Evaluation of Kidney Regeneration by Organ Scaffold Recellularization

Biomaterials and Their Biomedical Applications: From Replacement to Regeneration

Sustained releasing sponge-like 3D scaffolds for bone tissue engineering applications

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