Tissue engineering for bone defect healing: An update on a multi-component approach

Drosse, Inga; Volkmer, Elias; Capanna, Rodolfo; Biase, Pietro De; Mutschler, Wolf; Schieker, Matthias

doi:10.1016/s0020-1383(08)70011-1

Cited by 191 publications

(163 citation statements)

References 103 publications

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“…Despite the advantages of the technique, harvesting autogenous bone graft is an invasive method that often results in patient morbidity. Limited bone volume is another disadvantage of grafting, especially for large-scale ablative surgery [2]. Alternative methods are thus necessary to overcome this problem.…”

Section: Introductionmentioning

confidence: 99%

Composition of chitosan-hydroxyapatite-collagen composite scaffold evaluation after simulated body fluid immersion as reconstruction material

Verisqa

Triaminingsih

Corputty

2017

J. Phys.: Conf. Ser.

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Abstract. Hydroxyapatite (HA) formation is one of the most important aspects of bone regeneration. Because domestically made chitosan-hydroxyapatite-collagen composite scaffolding from crab shell and bovine bone and tendon has potential as a maxillofacial reconstruction material, the material's HA-forming ability requires evaluation. The aim of this research is to investigate chitosan-hydroxyapatite-collagen composite scaffold's potential as a maxillofacial reconstruction material by observing the scaffold's compositional changes. Scaffold specimens were immersed in 37°C simulated body fluid (SBF) for periods of 2, 4, 6, and 8 days. Scaffold composition was then evaluated by using energy dispersive spectroscopy (EDS). The calcium (Ca) and phosphorus (P) percentages of the scaffold were found to increase following SBF immersion. The high Ca/P ratio (3.82) on the scaffold indicated HA formation. Ion exchange played a significant role in the increased percentages of Ca and P, which led to new HA layer formation. The scaffold's HA acted as a nucleation site of Ca and P from the SBF, with collagen and chitosan as the scaffold's matrix. Chitosan-hydroxyapatitecollagen composite scaffold shows potential as a maxillofacial reconstruction material, since its composition favors HA formation.

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Section: Introductionmentioning

confidence: 99%

Composition of chitosan-hydroxyapatite-collagen composite scaffold evaluation after simulated body fluid immersion as reconstruction material

Verisqa

Triaminingsih

Corputty

2017

J. Phys.: Conf. Ser.

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“…In this sense, tissue 16 engineering (TE) (Fisher and Mauck, 2013;Langer and Vacanti, 17 1993) has played a key role as it has introduced new strategies 18 based on the development of composite systems that integrate 19 cells, growth factors, and scaffolds, (Drosse et al, 2008;Eisenbarth, 20 2007; Vallet-Regí and Ruiz-Hernández, 2011) avoiding major 21 drawbacks associated to autografts and allografts (O'Brien, 2011). 22 TE develops substrates that restore, maintain or improve the 23 function of damaged tissues (Langer, 2000).…”

Section: Introductionmentioning

confidence: 99%

Tuning dual-drug release from composite scaffolds for bone regeneration

Paris

Román

Manzano

et al. 2015

International Journal of Pharmaceutics

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Keywords:Dual-drug delivery system Designed porous scaffold Ibuprofen Zoledronic acid Co-delivery release Tissue engineering A B S T R A C TThis work presents the tuning of drug-loaded scaffolds for bone regeneration as dual-drug delivery systems. Two therapeutic substances, zoledronic acid (anti-osteoporotic drug) and ibuprofen (antiinflammatory drug) were successfully incorporated in a controlled manner into three dimensional designed porous scaffolds of apatite/agarose composite. A high-performance liquid chromatography method was optimized to separate and simultaneously quantify the two drugs released from the dualdrug codelivery system. The multifunctional porous scaffolds fabricated show a very rapid delivery of anti-inflammatory (interesting to reduce inflammation after implantation), whereas the antiosteoporotic drug showed sustained release behaviour (important to promote bone regeneration). Since ibuprofen release was faster than desired, this drug was encapsulated in chitosan spheres which were then incorporated into the scaffolds, obtaining a release profile suitable for clinical application. The results obtained open the possibility to simultaneously incorporate two or more drugs to an osseous implant in a controlled way improving it for bone healing application.2015 Published by Elsevier B.V. 7

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“…Although biological grafts such as autografts, allografts, or xenografts have advantages in biological bone regeneration, their wider application is limited due to histocompatibility, restricted supply, and secondary surgery (Jung et al, 2005). The foundation and rapid development of tissue engineering provides a possible strategy to reconstruct artificial tissue with a three-dimensional (3D) scaffold of biomaterials seeded with cells obtained from a small piece of tissue from the patient (Vacanti et al, 1993;Fuchs et al, 2003;Drosse et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, further studies on the application of bone tissue engineering with this composite scaffold were undertaken. According to Drosse et al (2008), the application of growth factors and osteogenic cells has been recommended to improve the recovery quality of large segmental bony defects. Thus, a new therapeutic strategy of nanocomposite scaffolds of g-HA/PLGA carried with autologous MSCs and bone morphogenetic protein-2 (BMP-2) was assessed for the therapy of critical bone defects.…”

Section: Introductionmentioning

confidence: 99%

Tissue-engineered composite scaffold of poly(lactide-co-glycolide) and hydroxyapatite nanoparticles seeded with autologous mesenchymal stem cells for bone regeneration

Zhang

Wang

et al. 2017

J. Zhejiang Univ. Sci. B

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Abstract:Objective: A new therapeutic strategy using nanocomposite scaffolds of grafted hydroxyapatite (g-HA)/ poly(lactide-co-glycolide) (PLGA) carried with autologous mesenchymal stem cells (MSCs) and bone morphogenetic protein-2 (BMP-2) was assessed for the therapy of critical bone defects. At the same time, tissue response and in vivo mineralization of tissue-engineered implants were investigated. Methods: A composite scaffold of PLGA and g-HA was fabricated by the solvent casting and particulate-leaching method. The tissue-engineered implants were prepared by seeding the scaffolds with autologous bone marrow MSCs in vitro. Then, mineralization and osteogenesis were observed by intramuscular implantation, as well as the repair of the critical radius defects in rabbits. Results: After eight weeks post-surgery, scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) revealed that g-HA/PLGA had a better interface of tissue response and higher mineralization than PLGA. Apatite particles were formed and varied both in macropores and micropores of g-HA/PLGA. Computer radiographs and histological analysis revealed that there were more and more quickly formed new bone formations and better fusion in the bone defect areas of g-HA/PLGA at 2-8 weeks post-surgery. Typical bone synostosis between the implant and bone tissue was found in g-HA/PLGA, while only fibrous tissues formed in PLGA. Conclusions: The incorporation of g-HA mainly improved mineralization and bone formation compared with PLGA. The application of MSCs can enhance bone formation and mineralization in PLGA scaffolds compared with cell-free scaffolds. Furthermore, it can accelerate the absorption of scaffolds compared with composite scaffolds.

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Tissue engineering for bone defect healing: An update on a multi-component approach

Cited by 191 publications

References 103 publications

Composition of chitosan-hydroxyapatite-collagen composite scaffold evaluation after simulated body fluid immersion as reconstruction material

Composition of chitosan-hydroxyapatite-collagen composite scaffold evaluation after simulated body fluid immersion as reconstruction material

Tuning dual-drug release from composite scaffolds for bone regeneration

Tissue-engineered composite scaffold of poly(lactide-co-glycolide) and hydroxyapatite nanoparticles seeded with autologous mesenchymal stem cells for bone regeneration

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