Tissue Engineering in Musculoskeletal Tissue: A Review of the Literature

Bove, Mary; Carlucci, Annalisa; Natale, Giovanni; Freda, Chiara; Noro, Antonio; Ferrara, Vincenzo; Opromolla, Giorgia; Martone, Mario; Cascone, Roberto; Messina, Gaetana; Izzo, Andrea; Vicidomini, Giovanni; Santini, Mario; Fiorelli, Alfonso

doi:10.3390/surgeries2010005

Cited by 5 publications

(3 citation statements)

References 198 publications

(175 reference statements)

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“…Examples of bioinert materials include some synthetic polymers and metals. Bioinert biomaterials are often used in applications where the biomaterial will not be resorbed or integrated into the surrounding tissue, such as orthopedic implants [49].…”

Section: Classification Based On Bioactivitymentioning

confidence: 99%

Applications of Biomaterials in Regenerative Medicine and Tissue Engineering – Concepts and Perspective

Najman

Stojanović

Živković

et al. 2023

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Regenerative medicine (RM) exploits the innate potential of the human body to effectively repair and regenerate damaged tissues and organs with the help of various biomaterials. Tissue engineering (TE) makes it possible to replace damaged tissues and organs with new ones. Research in the field of biomaterials has significantly improved the area of RM and TE. Biomaterials are used as orthopedic, dental, cardiovascular implants, medical devices, in the fields of reconstructive and regenerative medicine, among others. Important preconditions for the biomaterial to be used for implantation are its biocompatibility and biofunctionality. Biomaterials should enable adhesion, migration, proliferation and differentiation of cells. The biological properties of biomaterials are a reflection of their physicochemical properties, such as internal architecture, surface characteristics and charge. Biomaterials used in tissue regeneration should mimic the natural structure of the extracellular matrix and represent a physiological microenvironment for normal cellular functions. These biomaterials should also have adequate biodegradability properties to facilitate the formation and growth of new tissue. Biomaterials for use in RM can be of natural or synthetic origin, polymers by structural properties, ceramic and composite type, and based on bioreactivity they can be bioinert or bioactive. In RM and TE, polymers of different classes, natural and synthetic, are used, which can be made as intelligent materials. The structure of hydrogels in the form of a porous network represents a good matrix for cell activity. Ceramic biomaterials based on hydroxyapatite (HAp) are selected for use in RM and TE, especially solid tissues. Properties, such as composition, particle size, material shape, porosity, surface charge, topography, etc., are relevant for the proper use of HAp materials. The properties of HAp allow modification of its structure, surface, particle size design at the micro and nano level, hybridization with polymers, metals, etc. which is very important for its applications. Designed micro-nanohybrid HAp structure is most similar to the bone structure, making the cell environment closest to natural. Bone tissue engineering (BTE) is based on the combined use of cells, osteostimulating (osteoinductive) factors and biomaterials as a scaffolds and carriers for bone regeneration and defects repair. In BTE adipose-derived mesenchymal stem cells (ADSCs) are often used that are induced in vitro towards osteogenic cells or endothelial cells, and freshly isolated stromal vascular fraction can also be used. Blood components (PRP, blood plasma or blood clot) can be included in the composition of the BTE construct as a source of osteoinductive factors. In vitro models and methods were used to examine the biocompatibility, immunomodulatory and regenerative potential of biomaterials, as well as their influence on cellular functions. After in vitro methods, and before clinical studies, various in vivo animal models are used to examine the regenerative potential of biomaterials, such as subcutaneous implantations and bone defects in tibia, femur and calvaria in experimental animals (mouse, rat, rabbit).

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Section: Classification Based On Bioactivitymentioning

confidence: 99%

Applications of Biomaterials in Regenerative Medicine and Tissue Engineering – Concepts and Perspective

Najman

Stojanović

Živković

et al. 2023

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“…With its ability to repair, replace, and regenerate various types of biological tissues, tissue engineering is an enabling technology that holds significant potential in numerous biomedical applications. , Scaffolds are commonly used to serve as hosts for growing cells, guiding cell attachment, differentiation, proliferation, and migration for the development of new tissues . An ideal scaffold for the therapy of muscle injuries should: (1) possess good mechanical strength to provide additional support for skeletal muscles, (2) have excellent flexibility to conform to muscular geometry, (3) exhibit superior fatigue behavior to address the cyclic loading of muscles, (4) deliver adequate and sustainable antibiotics and analgesics to the target region for possible infection control/prophylaxis and pain relief, (5) be bioresorbable after fulfilling its intended function to avoid the need for a secondary surgery to remove the scaffold, and (6) be biocompatible to ensure that the process of material breakdown does not result in any tissue irritation.…”

Section: Introductionmentioning

confidence: 99%

Novel Bioresorbable Drug-Eluting Mesh Scaffold for Therapy of Muscle Injury

Chou,

Hsu,

Lee

et al. 2024

ACS Biomater. Sci. Eng.

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A novel bioresorbable drug-eluting polycaprolactone (PCL) mesh scaffold was developed, utilizing a solvent-cast additive manufacturing technique, to promote therapy of muscle injury. The degradation rate and mechanical properties strength of the PCL mesh were characterized after immersion in a buffer solution for different times. The in vitro release characteristics of vancomycin, ceftazidime, and lidocaine from the prepared mesh were evaluated using a highperformance liquid chromatography (HPLC) assay. In addition, the in vivo efficacy of PCL meshes for the repair of muscle injury was investigated on a rat model with histological examinations. It was found that the additively manufactured PCL meshes degraded by 13% after submission in buffered solution for four months. All PCL meshes with different pore sizes exhibited greater strength than rat muscle and survived through 10,000 cyclic loadings. Furthermore, the meshes could offer a sustained release of antibiotics and analgesics for more than 3 days in vitro. The results of this study suggest that drug-loaded PCL mesh exhibits superior ability to pure PCL mesh in terms of effectively promoting muscle repair in rat models. The histological assay also showed adequate biocompatibility of the resorbable meshes. The additively manufactured biodegradable drug-eluting meshes may be adopted in the future in humans for the therapy of muscle injuries.

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“…In addition to the regenerative approach, in vitro modeling for physiological and pathological studies of musculoskeletal tissue has also come into greater prominence recently. The 3D-bioprinted in vitro platforms, such as spheroids, organoids, and organ-on-chips containing multiple components, would benefit tissue modeling through drug discovery and the development of precise medicine and patient-specific regenerative therapies [20,21].…”

Section: Introductionmentioning

confidence: 99%

Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

Park

Gao

Cho

2021

IJMS

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The musculoskeletal system is a vital body system that protects internal organs, supports locomotion, and maintains homeostatic function. Unfortunately, musculoskeletal disorders are the leading cause of disability worldwide. Although implant surgeries using autografts, allografts, and xenografts have been conducted, several adverse effects, including donor site morbidity and immunoreaction, exist. To overcome these limitations, various biomedical engineering approaches have been proposed based on an understanding of the complexity of human musculoskeletal tissue. In this review, the leading edge of musculoskeletal tissue engineering using 3D bioprinting technology and musculoskeletal tissue-derived decellularized extracellular matrix bioink is described. In particular, studies on in vivo regeneration and in vitro modeling of musculoskeletal tissue have been focused on. Lastly, the current breakthroughs, limitations, and future perspectives are described.

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Tissue Engineering in Musculoskeletal Tissue: A Review of the Literature

Cited by 5 publications

References 198 publications

Applications of Biomaterials in Regenerative Medicine and Tissue Engineering – Concepts and Perspective

Applications of Biomaterials in Regenerative Medicine and Tissue Engineering – Concepts and Perspective

Novel Bioresorbable Drug-Eluting Mesh Scaffold for Therapy of Muscle Injury

Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

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