Tissue Engineering Meniscus

Ibarra, Clemente; Koski, John A.; Warren, Russell F.

doi:10.1016/s0030-5898(05)70160-7

Cited by 85 publications

(53 citation statements)

References 31 publications

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“…[21][22][23] Tissue engineering has recently been proposed as a possible solution for meniscal regeneration. 15,[24][25][26][27][28] There have, however, been very few animal studies reported in the literature that have investigated the possibility of using cells in a partial or total meniscal substitute. 15, 24 Walsh et al 25 used a collagenous sponge loaded with mesenchymal stem cells to heal a partial meniscus defect in rabbits and reported that the presence of cells augmented the repair process but did not prevent degenerative osteoarthritis.…”

Section: Introduction Lmentioning

confidence: 99%

Tissue Engineering for Total Meniscal Substitution: Animal Study in Sheep Model

Kon

Chiari

Marcacci

et al. 2008

Tissue Engineering Part A

113

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Objective: The aim of the study was to investigate the use of a novel hyaluronic acid/polycaprolactone material for meniscal tissue engineering and to evaluate the tissue regeneration after the augmentation of the implant with expanded autologous chondrocytes. Two different surgical implantation techniques in a sheep model were evaluated. Methods: Twenty-four skeletally mature sheep were treated with total medial meniscus replacements, while two meniscectomies served as empty controls. The animals were divided into two groups: cell-free scaffold and scaffold seeded with autologous chondrocytes. Two different surgical techniques were compared: in 12 animals, the implant was sutured to the capsule and to the meniscal ligament; in the other 12 animals, also a transtibial fixation of the horns was used. The animals were euthanized after 4 months. The specimens were assessed by gross inspection and histology.

show abstract

Section: Introduction Lmentioning

confidence: 99%

Tissue Engineering for Total Meniscal Substitution: Animal Study in Sheep Model

Kon

Chiari

Marcacci

et al. 2008

Tissue Engineering Part A

113

View full text Add to dashboard Cite

show abstract

“…[12][13][14][15][16][17][18][19][20][21][22][23][24][25] Various types of biomaterials including fibrin, collagen, and polyglycolic acid (PGA) have been used as scaffolds for meniscal tissue engineering. Histological results of partial meniscal defects filled with bone marrow cells mixed with fibrin glue showed mature healing of the defects.…”

Section: Introductionmentioning

confidence: 99%

Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model

Kang

Son

Lee

et al. 2006

J Biomedical Materials Res

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Abstract:The current treatments of meniscal lesion in knee joint are not perfect to prevent adverse effects of meniscus injury. Tissue engineering of meniscus using meniscal cells and polymer scaffolds could be an alternative option to treat meniscus injury. This study reports on the regeneration of whole medial meniscus in a rabbit total meniscectomy model using the tissue engineering technique. Biodegradable scaffolds in a meniscal shape were fabricated from polyglycolic acid (PGA) fiber meshes that were mechanically reinforced by bonding PGA fibers at cross points with 75:25 poly(lactic-co-glycolic acid). The compressive modulus of the bonded PGA scaffold was 28-fold higher than that of nonbonded scaffold. Allogeneic meniscal cells were isolated from rabbit meniscus biopsy and cultured in vitro. The expanded meniscal cells were seeded onto the polymer scaffolds, cultured in vitro for 1 week, and transplanted to rabbit knee joints from which medial menisci were removed. Ten or 36 weeks after transplantation, the implants formed neomenisci with the original scaffold shape maintained approximately. Hematoxylin and eosin staining of the sections of the neomenisci at 6 and 10 weeks revealed the regeneration of fibrocartilage. Safranin-O staining showed that abundant proteoglycan was present in the neomenisci at 10 weeks. Masson's trichrome staining indicated the presence of collagen. Immunohistochemical analysis showed that the presence of type I and II collagen in neomenisci at 10 weeks was similar to that of normal meniscal tissue. Biochemical and biomechanical analyses of the tissue-engineered menisci at 36 weeks were performed to determine the quality of the tissue-engineered menisci. Tissue-engineered meniscus showed differences in collagen content and aggregate modulus in comparison with native meniscus. This study demonstrates, for the first time, the feasibility of regenerating whole meniscal cartilage in a rabbit total meniscectomy model using the tissue engineering method.

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“…Tissue engineering or fabricating tissues is a discipline which involves replacement of anatomic structure of the damaged, injured, or missing tissue or organs by agglomerating biomaterials, cells, and biologically-active molecules [69][70][71] materials are 3-D scaffolds which provide mechanical support to the growing cells or tissue, guiding them in a microenvironment, mimicking the biological system. These can then be transplanted into the system.…”

Section: Carbon Nanotubes As Scaffold For Fabricating Tissuesmentioning

confidence: 99%