Tissue Engineering of Bladder Using Vascular Endothelial Growth Factor Gene-Modified Endothelial Progenitor Cells

Chen, Bai-Song; Xie, Hua; Zhang, Shengli; Geng, Hui; Zhou, Junmei; Pan, Jun; Chen, Fang

doi:10.5301/ijao.5000069

Cited by 32 publications

(14 citation statements)

References 27 publications

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“…Sharma et al also demonstrated that co-transplantation of CD34+ hematopoietic stem/progenitor cells and MSCs resulted in improved and de novo vascularization and peripheral nerve growth in the grafts [37]. Bladder acellular matrix grafts seeded with endothelial progenitor cells modified to express VEGF exhibited enhanced neovascularization in a porcine model of partial cystectomy, suggesting that cell seeding of grafts combined with VEGF gene therapy may be the future of bladder engineering [45]. …”

Section: Bladder Engineeringmentioning

confidence: 99%

The Current Use of Stem Cells in Bladder Tissue Regeneration and Bioengineering

et al. 2017

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Many pathological processes including neurogenic bladder and malignancy necessitate bladder reconstruction, which is currently performed using intestinal tissue. The use of intestinal tissue, however, subjects patients to metabolic abnormalities, bladder stones, and other long-term sequelae, raising the need for a source of safe and reliable bladder tissue. Advancements in stem cell biology have catapulted stem cells to the center of many current tissue regeneration and bioengineering strategies. This review presents the recent advancements in the use of stem cells in bladder tissue bioengineering.

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Section: Bladder Engineeringmentioning

confidence: 99%

The Current Use of Stem Cells in Bladder Tissue Regeneration and Bioengineering

et al. 2017

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“…As noted above, a number of studies have explored strategies to ensure appropriate neovascularization, either by optimization of processing strategies to retain bioactive factors in acellular scaffolds or by addition of recombinant angiogenic factors such as FGF-2 or VEGF [95, 106, 108]. In addition to covalent linkage of VEGF recombinant proteins, some investigators have incorporated VEGF plasmid into cells by viral transduction prior to seeding on scaffolds [109, 110] to ensure sustained production of protein following engraftment. These types of experiments exemplify dynamic reciprocity in that the scaffold ECM signals to endothelial cells in the host tissue, which in turn responds by undergoing angiogenesis to vascularize the graft and promote integration into the host organ.…”

Section: Modulation Of Scaffold Properties With Trophic Factorsmentioning

confidence: 99%

Dynamic reciprocity in cell–scaffold interactions

Mauney

Adam

2015

Advanced Drug Delivery Reviews

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Tissue engineering in urology has shown considerable promise. However, there is still much to understand, particularly regarding the interactions between scaffolds and their host environment, how these interactions regulate regeneration and how they may be enhanced for optimal tissue repair. In this review, we discuss the concept of dynamic reciprocity as applied to tissue engineering, i.e. how bi-directional signaling between implanted scaffolds and host tissues such as the bladder drives the process of constructive remodeling to ensure successful graft integration and tissue repair. The impact of scaffold content and configuration, the contribution of endogenous and exogenous bioactive factors, the influence of the host immune response and the functional interaction with mechanical stimulation are all considered. In addition, the temporal relationships of host tissue ingrowth, bioactive factor mobilization, scaffold degradation and immune cell infiltration, as well as the reciprocal signaling between discrete cell types and scaffolds are discussed. Improved understanding of these aspects of tissue repair will identify opportunities for optimization of repair that could be exploited to enhance regenerative medicine strategies for urology in future studies.

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“…Cells have been genetically modified for growth factor secretion (e.g., BMP, VEGF, and NGF) ex vivo , seeded on or within naturally-derived scaffolds, and subsequently implanted. 21,37,49,108 There is recent interest in the use of genetically modified mesenchymal stem cells (MSCs) to aid in the transplantation of xenogeneic materials. 73 When delivered in the absence of a scaffold, galactosyl epitope knock-out (Gal-KO) pig MSCs attenuated immune response and improved bone regeneration.…”

Section: Biological Propertiesmentioning

confidence: 99%

Functional Augmentation of Naturally-Derived Materials for Tissue Regeneration

Allen

Priddy

et al. 2014

Ann Biomed Eng

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Tissue engineering strategies have utilized a wide spectrum of synthetic and naturally-derived scaffold materials. Synthetic scaffolds are better defined and offer the ability to precisely and reproducibly control their properties, while naturally-derived scaffolds typically have inherent biological and structural properties that may facilitate tissue growth and remodeling. More recently, efforts to design optimized biomaterial scaffolds have blurred the line between these two approaches. Naturally-derived scaffolds can be engineered through the manipulation of intrinsic properties of the pre-existing backbone (e.g., structural properties), as well as the addition of controllable functional components (e.g., biological properties). Chemical and physical processing techniques used to modify structural properties of synthetic scaffolds have been tailored and applied to naturally-derived materials. Such strategies include manipulation of mechanical properties, degradation, and porosity. Furthermore, bio-functional augmentation of natural scaffolds via incorporation of exogenous cells, proteins, peptides, or genes has been shown to enhance functional regeneration over endogenous response to the material itself. Moving forward, the regenerative mode of action of naturally-derived materials requires additional investigation. Elucidating such mechanisms will allow for the determination of critical design parameters to further enhance efficacy and capitalize on the full potential of naturally-derived scaffolds.

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Tissue Engineering of Bladder Using Vascular Endothelial Growth Factor Gene-Modified Endothelial Progenitor Cells

Cited by 32 publications

References 27 publications

The Current Use of Stem Cells in Bladder Tissue Regeneration and Bioengineering

The Current Use of Stem Cells in Bladder Tissue Regeneration and Bioengineering

Dynamic reciprocity in cell–scaffold interactions

Functional Augmentation of Naturally-Derived Materials for Tissue Regeneration

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