Tissue Engineering of Flexor Tendons: The Effect of a Tissue Bioreactor on Adipoderived Stem Cell–Seeded and Fibroblast-Seeded Tendon Constructs

Angelidis, Ioannis K.; Thorfinn, Johan; Connolly, Ian D.; Lindsey, Derek P.; Pham, Hung; Chang, James

doi:10.1016/j.jhsa.2010.06.020

Cited by 52 publications

(52 citation statements)

References 24 publications

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“…The mechanical stimulation induced longitudinal alignment of BMSCs in the direction of stretch. The morphology of BMSCs was similar to that of native tenocytes in situ [13,32], with cells oriented parallel to the collagen fibers, elongated and engrafted into the scaffold, and able to migrate through the ECM under dynamic stretch culture. The cells were distributed randomly but developed a longitudinal pattern following stretching, which is consistent with the finding that has been observed previously using two-dimensional cell stretching experiments [33,34].…”

Section: Discussionmentioning

confidence: 82%

“…Such bioreactor stimulation has been shown to improve biomechanical properties in decellularized tendon scaffolds reseeded with rabbit tenocytes [12]. However, distribution of the reseeded cells was limited to the surface, with far fewer cells in the core of the scaffold [13]. Whitlock et al [14] increased the porosity and pore size of the tendon scaffold by using a combined solution of TritonX-100 and peracetic acid.…”

Section: Introductionmentioning

confidence: 99%

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Effect of mechanical stimulation on bone marrow stromal cell–seeded tendon slice constructs: A potential engineered tendon patch for rotator cuff repair

et al. 2015

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Cell-based tissue engineered tendons have potential to improve clinical outcomes following rotator cuff repair, especially in large or massive rotator cuff tears, which pose a great clinical challenge. The aim of this study was to develop a method of constructing a functional engineered tendon patch for rotator cuff repair with cyclic mechanical stimulation. Decellularized tendon slices (DTSs) were seeded with BMSCs and subjected to cyclic stretching for 1, 3, or 7 days. The mechanical properties, morphologic characteristics and tendon-related gene expression of the constructs were investigated. Viable BMSCs were observed on the DTS after 7 days. BMSCs penetrated into the DTSs and formed dense cell sheets after 7 days of mechanical stretching. Gene expression of type I collagen, decorin, and tenomodulin significantly increased in cyclically stretched BMSC-DTS constructs compared with the unstrained control group (P < 0.05). The ultimate tensile strength and stiffness of the cyclically stretched tendon constructs were similar to the unstrained control group (P > 0.05). In conclusion, mechanical stimulation of BMSC-DTS constructs upregulated expression of tendon-related proteins, promoted cell tenogenic differentiation, facilitated cell infiltration and formation of cell sheets, and retained mechanical properties. The patch could be used as a graft to enhance the surgical repair of rotator cuff tears.

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Section: Discussionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Effect of mechanical stimulation on bone marrow stromal cell–seeded tendon slice constructs: A potential engineered tendon patch for rotator cuff repair

et al. 2015

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“…Human ligaments were engineered from human MSCs in a bioreactor by combining dynamic tension and torsion mimicking forces in vivo, which significantly improved the ligament function [64] . Using MSCs and fibroblasts seeded in tendon constructs in a bioreactor with cyclic mechanical load, tendons with comparable ultimate tensile stress and elastic modulus to fresh tendons were obtained [65] . Different cell types, such as MSCs and human EB-derived mesenchymal progenitors, may respond differently to the same mechanical force in a mechanical compression bioreactor [66] .…”

Section: Mechanical Stimulationmentioning

confidence: 99%

Engineering stem cell niches in bioreactors

Liu

Liu²,

Zang³

et al. 2013

WJSC

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Stem cells, including embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells and amniotic fluid stem cells have the potential to be expanded and differentiated into various cell types in the body. Efficient differentiation of stem cells with the desired tissue-specific function is critical for stem cell-based cell therapy, tissue engineering, drug discovery and disease modeling. Bioreactors provide a great platform to regulate the stem cell microenvironment, known as "niches", to impact stem cell fate decision. The niche factors include the regulatory factors such as oxygen, extracellular matrix (synthetic and decellularized), paracrine/ autocrine signaling and physical forces (i.e. , mechanical force, electrical force and flow shear). The use of novel bioreactors with precise control and recapitulation of niche factors through modulating reactor operation parameters can enable efficient stem cell expansion and differentiation. Recently, the development of microfluidic devices and microbioreactors also provides powerful tools to manipulate the stem cell microenvironment by adjusting flow rate and cytokine gradients. In general, bioreactor engineering can be used to better modulate stem cell niches critical for stem cell expansion, differentiation and applications as novel cell-based biomedicines. This paper reviews important factors that can be more precisely controlled in bioreactors and their effects on stem cell engineering.

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“…After a bioreactor pretreatment, the ASCs contributed to the in vitro production of strong tendon material. 10 ASCs also can differentiate into osteocytes and chondrocytes. 11 Monaco et al 12 reported that biomaterial scaffolds in combination with ASCs and growth factors provide a valuable tool for guided bone regeneration, especially for complex anatomic defects.…”

Section: De Mattos Carvalhomentioning

confidence: 99%

Adipogenesis of Human Adipose-Derived Stem Cells Within Three-Dimensional Hollow Fiber-Based Bioreactors

Gerlach

Lin²,

Brayfield³

et al. 2012

Tissue Engineering Part C: Methods

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Tissue Engineering of Flexor Tendons: The Effect of a Tissue Bioreactor on Adipoderived Stem Cell–Seeded and Fibroblast-Seeded Tendon Constructs

Cited by 52 publications

References 24 publications

Effect of mechanical stimulation on bone marrow stromal cell–seeded tendon slice constructs: A potential engineered tendon patch for rotator cuff repair

Effect of mechanical stimulation on bone marrow stromal cell–seeded tendon slice constructs: A potential engineered tendon patch for rotator cuff repair

Engineering stem cell niches in bioreactors

Adipogenesis of Human Adipose-Derived Stem Cells Within Three-Dimensional Hollow Fiber-Based Bioreactors

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