Tissue expressions of nine genes important to immune defence of the Pacific white shrimp Litopenaeus vannamei

Wang, Yu-Chi; Chang, Poh-Shing; Chen, Houng-Yung

doi:10.1016/j.fsi.2007.04.004

Cited by 115 publications

(58 citation statements)

References 66 publications

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“…4). In the present study, real-time PCR analysis revealed that MNHc was mainly expressed in hepatopancreas, which is consistent with the results of Litopenaeus vannamei [13] . As early as 1970s, Gibson et al [14] put forward that hepatopancreas is the main organ of crustaceans to synthesize hemocyanin.…”

Section: Discussionsupporting

confidence: 92%

Cloning and characterization of the hemocyanin gene of prawn Macrobrachium nipponense

Wang¹,

Xia²,

Liu³

et al. 2012

Turk J Bioch

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Section: Discussionsupporting

confidence: 92%

Cloning and characterization of the hemocyanin gene of prawn Macrobrachium nipponense

Wang¹,

Xia²,

Liu³

et al. 2012

Turk J Bioch

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“…As GSP contains b-1, 3-glycosidic linkages and b-1, 6-glycosidic linkages, it has multiple bioactive functions. From the shrimp L. vannamei, Cheng et al had cloned the lipopolysaccharide and b-1, 3-glucan binding protein (LGBP) [33], and Romo-Figueroa et al had cloned the high density lipoprotein/b-1, 3-glucan binding protein (HDL/BGBP) [34], LGBP and HDL/BGBP both are pattern recognition proteins (PRPs) that play critical roles in the activation of the proPO system, coagulation process and expression of antimicrobial peptides after recognition of microbial components [35,36]. b-glucan consists of b-1, 3-linked glucosyl residues with 95% glucose [37], it can mediate the immune activity of L. vannamei and up-regulate mRNA expression of LGBP and HDL/BGBP [3], increase activity of anti-oxidant enzymes in grass carp [38], and modulate the antibacterial activity of Ruditapes decussatus and Mytilus galloprovincialis [39].…”

Section: Discussionmentioning

confidence: 99%

The effect of dietary Panax ginseng polysaccharide extract on the immune responses in white shrimp, Litopenaeus vannamei

Liu

Yang

et al. 2011

Fish & Shellfish Immunology

167

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“…Las secuencias obtenidas de los seis genes se compararon con las secuencias nucleotídicas reportadas en el banco genómico (GeneBank), coincidiendo con las reportadas por Wang et al (2007).…”

Section: Análisis De La Expresión De Genes Del Sistema Inmuneunclassified

“…En los camarones, los mecanismos celulares (citotoxicidad, coagulación, encapsulación, fagocitosis, nodulación y melanización) y humorales (enzimas lisosomales, lectinas, peroxidasa, proteínas de la coagulación, péptidos antimicrobianos y radicales libres del oxígeno y el nitrógeno) contribuyen a la defensa del organismo, limitando invasiones microbianas y virales que son eliminadas de la hemolinfa y tejidos (Söderhäll & Cerenius, 1992;Sritunyalucksana et al, 2001;Bachère et al, 2004;Cerenius et al, 2008). La expresión de genes relacionados con el sistema inmune en el camarón proporciona información importante sobre la activación del sistema inmune y su modulación (Wang et al, 2007).…”

Section: Introductionunclassified

Respuesta inmune y expresion de genes en el camaron blanco (Litopenaeus vannamei) inducida por inmunoestimulantes microbianos

Luna‐González¹,

Moreno-Herrera²,

Campa‐Córdova³

et al. 2013

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RESUMEN.Se realizó un bioensayo de 26 días para evaluar el efecto inmunoestimulante de bacterias ácido lácticas y levaduras (MI), adicionadas en el alimento, en Litopenaeus vannamei. Los tratamientos del bioensayo se realizaron por triplicado: I) dieta control (Camaronina ® ); II) MI en alimento, diario; III) MI en alimento, cada tres días y; IV) MI en alimento, cada seis días. Los camarones sólo eran libres de WSSV. Para el estudio del sistema inmune se hizo un conteo total de hemocitos, se determinó bioquímicamente la concentración de anión superóxido, y la actividad de la fenoloxidasa. También se estudió la expresión semicuantitativa de seis genes del sistema inmune, utilizando la técnica de RT-PCR. No hubo aumento significativo en el crecimiento y la supervivencia, el conteo total de hemocitos, la concentración de la proteína total en plasma y hemocitos, y la concentración del anión superóxido. La actividad de la fenoloxidasa en plasma en el tratamiento IV fue significativamente mayor que en los tratamientos I, II y III. La fenoloxidasa del SLH (proFO) en el tratamiento IV fue significativamente mayor que en los tratamientos I y III. La MI provocó una sobreexpresión significativa de los genes que codifican para la profenoloxidasa (tratamiento IV), lisozima (tratamiento III) y transglutaminasa (tratamiento II), con respecto a los animales no tratados (control). La mezcla de inmunoestimulantes microbianos puede aumentar la resistencia de L. vannamei contra patógenos en los cultivos. Palabras clave: expresión de genes, inmunoestimulantes microbianos, Litopenaeus vannamei. Immune response and gene expression of white shrimp (Litopenaeus vannamei)induced by microbial immunostimulants ABSTRACT. A 26-day bioassay was performed to evaluate the immunostimulating effect of lactic acid bacteria and yeasts (IM), added in the feed, in Litopenaeus vannamei. Bioassay treatments were performed in triplicate: I) control diet (Camaronina ® ); II) IM in food, daily; III) IM in food every three days and; IV) IM in food every six days. The shrimp were WSSV-free. To study the immune system, a total count of hemocytes, the concentration of superoxide anion, and the phenoloxidase activity were analyzed. We also studied the semiquantitative expression of six immune system genes, using the technique of RT-PCR. There was no significant increase in the growth and survival, the total count of hemocytes, the protein concentration, and the concentration of superoxide anion. Phenoloxidase activity in plasma in the treatment IV was significantly higher than in treatments I, II, and III. The phenoloxidase of HLS (proPO) in the IV treatment was significantly higher than in treatments I and III. The IM caused a significant overexpression of the genes coding for the prophenoloxidase (treatment IV), lysozyme (treatment III), and transglutaminase (treatment II) relative to the untreated animals (control). The mixture of microbial immunostimulants may increase the resistance of cultured L. vannamei against pathogens.

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Tissue expressions of nine genes important to immune defence of the Pacific white shrimp Litopenaeus vannamei

Cited by 115 publications

References 66 publications

Cloning and characterization of the hemocyanin gene of prawn Macrobrachium nipponense

Cloning and characterization of the hemocyanin gene of prawn Macrobrachium nipponense

The effect of dietary Panax ginseng polysaccharide extract on the immune responses in white shrimp, Litopenaeus vannamei

Respuesta inmune y expresion de genes en el camaron blanco (Litopenaeus vannamei) inducida por inmunoestimulantes microbianos

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