Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver

Appelmans, F.; Wattiaux, Robert; Duve, Christian de

doi:10.1042/bj0590438

Cited by 601 publications

(164 citation statements)

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“…In the late 1950s, de Duve and his collaborators discovered lysosomes. 37,38 The discovery was accidental: they had developed the technique of differential centrifugation and, having left the fractions of a liver homogenate overnight rather than processing them immediately, they found much more acid phosphatase in the 'mitochondrial' fraction. They quickly determined that what we now know as primary lysosomes had ruptured, releasing acid phosphatase and other acid hydrolases into the supernatant.…”

Section: Open Questionsmentioning

confidence: 99%

Programmed cell death 50 (and beyond)

Lockshin

2015

Cell Death Differ

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In the 50 years since we described cell death as 'programmed,' we have come far, thanks to the efforts of many brilliant researchers, and we now understand the mechanics, the biochemistry, and the genetics of many of the ways in which cells can die. This knowledge gives us the resources to alter the fates of many cells. However, not all cells respond similarly to the same stimulus, in either sensitivity to the stimulus or timing of the response. Cells prevented from dying through one pathway may survive, survive in a crippled state, or die following a different pathway. To fully capitalize on our knowledge of cell death, we need to understand much more about how cells are targeted to die and what aspects of the history, metabolism, or resources available to individual cells determine how each cell reaches and crosses the threshold at which it commits to death.

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Section: Open Questionsmentioning

confidence: 99%

Programmed cell death 50 (and beyond)

Lockshin

2015

Cell Death Differ

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“…Because of the possibilities to perform a morphometric analysis as well on intact tissue as on fractions and homogenate, it should be possible to extend the calculation of recoveries to include all three preparations, i.e., to estimate how well the membranes found in intact cells are recovered in homogenate and fractions. This paper describes the methods required to collect and analyze the stereological data and presents the recoveries obtained by using analytical cell fractionation on rat liver tissue (1).…”

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confidence: 99%

Intergrated stereological and biochemical studies of hepatocytic membranes. I. Membrane recoveries in subcellular fractions

Bolender¹,

Paumgartner²,

Losa³

et al. 1978

The Journal of Cell Biology

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Previous attempts to relate the structure and function of hepatocytic membranes have compared biochemical data of fractions to morphological data derived from either intact tissue or fractions. The effects of the original homogenization aside, biochemical recoveries comparing membrane marker enzymes of the homogenate to subsequent fractions suggest a general conservation of activity. A stereological study was undertaken to estimate membrane surface areas in the intact tissue, homogenate, and fractions of the same livers and then to test the comparability of these data with membrane marker enzymes by calculating both morphological and biochemical recoveries. The stereological data were corrected for errors due to section thickness and compression.

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“…We characterized the microsomal fraction isolated from bovine pulmonary artery smooth muscle tissue by measuring at different steps in the preparation process the activity of cytochrome c oxidase, a mitochondrial marker [29], of acid phosphatase, a lysosomal marker [30], of NADPH-Cyt. c re-S. I~ Ghosh et al/FEBS Letters 387 (1996) 171-174 Fig.…”

Section: Resultsmentioning

confidence: 99%

Oxidant‐mediated proteolytic activation of Ca²⁺‐ATPase in microsomes of pulmonary smooth muscle

et al. 1996

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Treatment of bovine pulmonary artery smooth muscle tissue microsomes with H202 (l mM) markedly stimulated protease activity tested with a synthetic substrate N-benzoyl-DLarginine p-nitroanilide (BAPNA), and also enhanced Ca 2+-ArPase activity. ATP-dependent Ca 2+ uptake was found to be stimulated upon treatment of the microsomes with n202.Pretreatment of the microsomes with vitamin E and aprotinin prevented the H202-induced stimulation of Ca2+-ATPase activity and also ATP-dependent Ca 2+ uptake. In contrast, H,O2-induced inhibition of Na+-dependent Ca 2+ uptake was reversed by vitamin E and aprotinin.

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Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver

Cited by 601 publications

References 13 publications

Programmed cell death 50 (and beyond)

Programmed cell death 50 (and beyond)

Intergrated stereological and biochemical studies of hepatocytic membranes. I. Membrane recoveries in subcellular fractions

Oxidant‐mediated proteolytic activation of Ca²⁺‐ATPase in microsomes of pulmonary smooth muscle

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Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver

Cited by 601 publications

References 13 publications

Programmed cell death 50 (and beyond)

Programmed cell death 50 (and beyond)

Intergrated stereological and biochemical studies of hepatocytic membranes. I. Membrane recoveries in subcellular fractions

Oxidant‐mediated proteolytic activation of Ca2+‐ATPase in microsomes of pulmonary smooth muscle

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Oxidant‐mediated proteolytic activation of Ca²⁺‐ATPase in microsomes of pulmonary smooth muscle