TissueRNFT2Expression Levels Are Associated With Peritoneal Recurrence and Poor Prognosis in Gastric Cancer

Abstract

Background/Aim: Disease recurrence is frequently observed after curative resection of advanced gastric cancer resulting in a poor prognosis. In the present study, we identified a candidate biomarker to predict recurrence and prognosis after curative resection of gastric cancer. Materials and Methods: A transcriptome analysis was conducted using surgically resected cancerous tissue from patients with metastatic gastric cancer to identify genes that are upregulated in primary and metastatic tissues. Results: Ring finger protein, transmembrane 2 (RNFT2) mRNA expression was upregulated in primary gastric cancer tissues and metastases compared with non-cancerous tissues. RNFT2 expression in gastric cancer cell lines was positively correlated with the EMT-related molecules GSC, MMP9, and RAC1. The RNFT2 high expression group exhibited a significantly shorter postoperative overall survival. Peritoneal recurrence was significantly higher in the RNFT2 high expression group. Conclusion: RNFT2 mRNA expression predicts peritoneal recurrence and is a potential prognostic biomarker for gastric cancer following curative gastrectomy.Gastric cancer has become the leading cause of cancerrelated deaths worldwide (1). Despite advances in our understanding of the pathogenesis and treatment of gastric cancer, the recurrence rate after radical gastrectomy remains high and prognosis is poor (2, 3). The identification of biomarkers that predict gastric cancer metastasis and recurrence will enable physicians to predict the risk of recurrence in individual patients (4, 5). Perioperative chemotherapy and postoperative follow-up, considering the respective risks, may contribute to the prevention of recurrence, early detection, and improved prognosis.The type of recurrence and metastasis of gastric cancer include lymph node recurrence, hematogenous metastasis and peritoneal dissemination. Among them, in particular peritoneal metastasis is difficult to detect early and systemic chemotherapy is largely ineffective. As a result, peritoneal metastasis is a major cause of poor prognosis in gastric cancer (6).Defining the risk of peritoneal metastasis is necessary for prediction and early detection, but there are currently no established biomarkers.To identify differentially expressed genes that associate with the metastasis of gastric cancer, we conducted a transcriptome analysis of gastric clinical specimens from patients with distant metastasis. We identified a ring finger protein, transmembrane 2 (RNFT2), as a novel candidate biomarker. RNFT2 has been implicated in mediating protein interactions and the ubiquitination of target proteins, but its role in gastric cancer has not been reported. In the present study, we investigated the relationship between RNFT2 mRNA expression in gastric cancer tissues and the likelihood of recurrence after curative gastrectomy. Materials and MethodsEthics. This study conformed to the ethical guidelines of the World Medical Association Declaration of Helsinki Ethical Principles for Medical Research Involving Human...

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