Tissue Imaging at Atmospheric Pressure Using Desorption Electrospray Ionization (DESI) Mass Spectrometry

Wiseman, Justin M.; Ifa, Demian R.; Song, Qingyu; Cooks, R. Graham

doi:10.1002/ange.200602449

Cited by 77 publications

(92 citation statements)

References 23 publications

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“…Sulfatide fragmentation described in the literature shows several complex processes [93,102,103] Table 1. These data are consistent with previously reported MS analysis of rat brain sections [45,80,89]. Although the sulfatide lipids are not the most abundant in rat spinal cord [21], they correspond to the main detected ions in the negative ion mass spectrum, their more efficient ionization being due to the high acidity of the sulfonic group.…”

Section: Identification Of Compoundssupporting

confidence: 92%

“…This so-called 'droplet pick-up' process is followed by the standard electrospray evaporation mechanisms which produce dry ions of the analytes [70,71]. DESI has been used to construct chemical images of tissue sections [72][73][74][75][76][77][78][79][80][81][82][83][84][85][86] and Scheme 1 in forensic analysis [87,88]. The spatial resolution of DESI is typically 200 m [74] although significantly higher resolution has been reported [75].…”

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confidence: 99%

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Desorption electrospray ionization imaging mass spectrometry of lipids in rat spinal cord

Girod

Shi

Cheng

et al. 2010

J. Am. Soc. Mass Spectrom.

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Imaging mass spectrometry allows for the direct investigation of tissue samples to identify specific biological compounds and determine their spatial distributions. Desorption electrospray ionization (DESI) mass spectrometry has been used for the imaging and analysis of rat spinal cord cross sections. Glycerophospholipids and sphingolipids, as well as fatty acids, were detected in both the negative and positive ion modes and identified through tandem mass spectrometry (MS/MS) product ion scans using collision-induced dissociation and accurate mass measurements. Differences in the relative abundances of lipids and free fatty acids were present between white and gray matter areas in both the negative and positive ion modes. DESI-MS images of the corresponding ions allow the determination of their spatial distributions within a cross section of the rat spinal cord, by scanning the DESI probe across the entire sample surface. Glycerophospholipids and sphingolipids were mostly detected in the white matter, while the free fatty acids were present in the gray matter. These results show parallels with reported distributions of lipids in studies of rat brain. This suggests that the spatial intensity distribution reflects relative concentration differences of the lipid and fatty acid compounds in the spinal cord tissue. The "butterfly" shape of the gray matter in the spinal cord cross section was resolved in the corresponding ion images, indicating that a lateral resolution of better than 200 m was achieved. The selected ion images of lipids are directly correlated with anatomic features on the spinal cord corresponding to the white and the gray

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Section: Identification Of Compoundssupporting

confidence: 92%

mentioning

confidence: 99%

Desorption electrospray ionization imaging mass spectrometry of lipids in rat spinal cord

Girod

Shi

Cheng

et al. 2010

J. Am. Soc. Mass Spectrom.

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“…The DESI ion source utilized in these studies was an early Prosolia Inc. prototype and was configured as described previously [30]. The DESI spray was positioned 2 mm from the surface at an incident angle of 54°a nd a low (ca.…”

Section: Mass Spectrometrymentioning

confidence: 99%

“…In particular, the intensities of certain sphingomyelin species marked the margin between the non-tumor and the tumor portion of the tissue sections. More recently, two-dimensional molecular ion images of thin sections of rat brain tissue have been recorded using 2D rastering of the cryosectioned tissue with a DESI ion source, and characteristically different distributions in lipids have been noted [30,31].…”

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confidence: 99%

Desorption electrospray ionization (DESI) mass spectrometry and tandem mass spectrometry (MS/MS) of phospholipids and sphingolipids: Ionization, adduct formation, and fragmentation

Manicke

Wiseman

Ifa

et al. 2008

J. Am. Soc. Mass Spectrom.

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Desorption electrospray ionization (DESI) mass spectrometry was evaluated for the characterization of glycerophospholipid standards, including glycerophosphocholine (GPCho), glycerophosphoglycerol (GPGro), glycerophosphoethanolamine (GPEtn), glycerophosphoserine (GPSer), glycerophosphoinositol (GPIns), cardiolipin (CL), and sphingolipid standards, including sulfatides (ST) and sphingomyelin (SM). Of specific interest were the effects of surface and solvent composition on signal stability and intensity, along with the ions observed in the full scan mode and the fragmentations seen upon collisional activation for each of the above classes. These experiments were performed without the addition of matrix compounds to the sample and were conducted in the free ambient environment at atmospheric pressure. The compounds GPSer, GPGro, GPIns, ST, and CL were best analyzed in the negative ion mode while PE was ionized efficiently in both positive and negative ion modes. SM and GPCho, which typically generate more abundant ions in the positive ion mode, could be analyzed in the negative ion mode by the addition of anionic reagents such as acetate to the spray solvent. Full scan DESI mass spectra and tandem (MS/MS) spectra for this representative set of physiological phospho/sphingolipids are presented. Similarities with other ionization methods in terms of fragmentation behavior were strong, although ambient ionization of untreated samples is only available with DESI. The effect of surface and solvent properties on signal intensity and stability were determined by depositing standard compounds on several different surfaces and analyzing with various proportions of methanol in the aqueous spray. Analysis was extended to complex mixtures of phospholipids and sphingolipids by examining the total lipid extract of porcine brain and by direct analysis of rat brain cryotome sections. These types of mixture analyses and molecular imaging studies are likely to represent major areas of application of DESI. (J

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“…But in principle any ion source capable to ionize molecules from solid samples is amenable to be used for this purpose. The most used instruments for this application are MALDI-TOF [51] and TOF-SIMS [52] spectrometers, although recently desorption ESI (DESI) has been use successfully for tissue imaging [53]. To generate images for the MS data, the whole surface of the sample is rastered by the laser or ion beam.…”

Section: Ms-imaging Of Atheroma Plaquesmentioning

confidence: 99%

Vascular proteomics

Vivanco

Mas

Dardé

et al. 2007

Proteomics Clinical Apps

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The characterization of patients with acute coronary syndromes (ACS) at the molecular and cellular levels provides a novel vision for understanding the pathological and clinical expression of the disease. Recent advances in proteomic technologies permit the evaluation of systematic changes in protein expression in many biological systems and have been extensively applied to cardiovascular diseases (CVD). The cardiovascular system is in permanent intimate contact with blood, making blood-based biomarker discovery a particularly worthwhile approach. Thus, proteomics can potentially yield novel biomarkers reflecting CVD, establish earlier detection strategies, and monitor response to therapy. Here we review the different proteomic strategies used in the study of atherosclerosis and the novel proteins differentially expressed and secreted by atherosclerotic lesions which constitute novel potential biomarkers (HSP-27, Cathepsin D). Special attention is paid to MS-Imaging of atheroma plaque and the generation, for the first time, of 2-D images of lipids, showing the distribution of these molecules in the different areas of the atherosclerotic lesions. In addition new potential biomarkers have been identified in plasma (amyloid A1a, transtherytin), circulating cells (protein profile in monocytes from ACS patients) and individual cells constituents of atheroma plaques (endothelial, VSMC, macrophages) which provide novel insights into vascular pathophysiology.

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Tissue Imaging at Atmospheric Pressure Using Desorption Electrospray Ionization (DESI) Mass Spectrometry

Cited by 77 publications

References 23 publications

Desorption electrospray ionization imaging mass spectrometry of lipids in rat spinal cord

Desorption electrospray ionization imaging mass spectrometry of lipids in rat spinal cord

Desorption electrospray ionization (DESI) mass spectrometry and tandem mass spectrometry (MS/MS) of phospholipids and sphingolipids: Ionization, adduct formation, and fragmentation

Vascular proteomics

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