Tissue ingrowth markedly reduces mechanical anisotropy and stiffness in fibre direction of highly aligned electrospun polyurethane scaffolds

Krynauw, Hugo; Buescher, Jannik; Koehne, Josepha; Verrijt, Loes; Limbert, Georges; Davies, Neil; Bezuidenhout, Deon; Franz, Thomas

doi:10.1101/779942

Cited by 2 publications

(4 citation statements)

References 29 publications

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“…Physical characterisation included microscopic analysis of fibre diameter and alignment, measurement of scaffold wall thickness and porosity as described previously [32].…”

Section: Physical Characterisation Of Scaffold Before Implantationmentioning

confidence: 99%

“…In previous studies, we separately quantified the effect of in vitro degradation [31] and in situ tissue ingrowth in a biostable scaffold [32] on the scaffolds' mechanical properties. The present study aimed at investigating the combined effects of concurrent in situ tissue ingrowth and scaffold degradation on the mechanical properties of a fast-degrading electrospun polyester-urethane scaffold.…”

Section: Introductionmentioning

confidence: 99%

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Electrospun polyester-urethane scaffold preserves mechanical properties and exhibits strain stiffening during in situ tissue ingrowth and degradation

et al. 2020

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Consistent mechanical performance from implantation through healing and scaffold degradation is highly desired for tissue-regenerative scaffolds, e.g. when used for vascular grafts. The aim of this study was the paired in vivo mechanical assessment of biostable and fast degrading electrospun polyester-urethane scaffolds to isolate the effects of material degradation and tissue formation after implantation. Biostable and degradable polyester-urethane scaffolds with substantial fibre alignment were manufactured by electrospinning. Scaffold samples were implanted paired in subcutaneous position in rats for 7, 14 and 28 days. Morphology, mechanical properties and tissue ingrowth of the scaffolds were assessed before implantation and after retrieval. Tissue ingrowth after 28 days was 83 ± 10% in the biostable scaffold and 77 ± 4% in the degradable scaffold. For the biostable scaffold, the elastic modulus at 12% strain increased significantly between 7 and 14 days and decreased significantly thereafter in fibre but not in cross-fibre direction. The degradable scaffold exhibited a significant increase in the elastic modulus at 12% strain from 7 to 14 days after which it did not decrease but remained at the same magnitude, both in fibre and in cross-fibre direction. Considering that the degradable scaffold loses its material strength predominantly during the first 14 days of hydrolytic degradation (as observed in our previous in vitro study), the consistency of the elastic modulus of the degradable scaffold after 14 days is an indication that the regenerated tissue construct retains its mechanical properties.

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“…Physical characterisation included microscopic analysis of fibre diameter and alignment, measurement of scaffold wall thickness and porosity as described previously [32].…”

Section: Physical Characterisation Of Scaffold Before Implantationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrospun polyester-urethane scaffold preserves mechanical properties and exhibits strain stiffening during in situ tissue ingrowth and degradation

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Physical characterisation included microscopic analysis of fibre diameter and alignment, measurement of scaffold wall thickness and porosity as described previously [18].…”

Section: Physical Characterisation Of Scaffold Before Implantationmentioning

confidence: 99%

“…In previous studies, we separately quantified the effect of in vitro degradation [17] and in situ tissue ingrowth in a biostable scaffold [18] on the scaffolds' mechanical properties. The present study aimed at investigating the combined effects of concurrent in situ tissue ingrowth and scaffold degradation on the mechanical properties of a fast-degrading electrospun polyester-urethane scaffold.…”

Section: Introductionmentioning

confidence: 99%

Electrospun polyester-urethane scaffold preserves mechanical properties and exhibits strain stiffening during in situ tissue ingrowth and degradation

Krynauw

Omar

Koehne

et al. 2019

Preprint

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AbstractConsistent mechanical performance from implantation through healing and scaffold degradation is highly desired for tissue-regenerative scaffolds, e.g. when used for vascular grafts. The aim of this study was the paired in vivo mechanical assessment of biostable and fast degrading electrospun polyester-urethane scaffolds to isolate the effects of material degradation and tissue formation after implantation. Biostable and degradable polyester-urethane scaffolds with substantial fibre alignment were manufactured by electrospinning. Scaffold samples were implanted paired in subcutaneous position in rats for 7, 14 and 28 days. Morphology, mechanical properties and tissue ingrowth of the scaffolds were assessed before implantation and after retrieval. Tissue ingrowth after 28 days was 83 ± 10% in the biostable scaffold and 77 ± 4% in the degradable scaffold. For the biostable scaffold, the elastic modulus at 12% strain increased significantly between 7 and 14 days and decreased significantly thereafter in fibre but not in cross-fibre direction. The degradable scaffold exhibited a significant increase in the elastic modulus at 12% strain from 7 to 14 days after which it did not decrease but remained at the same magnitude, both in fibre and in cross-fibre direction. Considering that the degradable scaffold loses its material strength predominantly during the first 14 days of hydrolytic degradation (as observed in our previous in vitro study), the consistency of the elastic modulus of the degradable scaffold after 14 days is an indication that the regenerated tissue construct retains it mechanical properties.

show abstract

Tissue ingrowth markedly reduces mechanical anisotropy and stiffness in fibre direction of highly aligned electrospun polyurethane scaffolds

Cited by 2 publications

References 29 publications

Electrospun polyester-urethane scaffold preserves mechanical properties and exhibits strain stiffening during in situ tissue ingrowth and degradation

Electrospun polyester-urethane scaffold preserves mechanical properties and exhibits strain stiffening during in situ tissue ingrowth and degradation

Electrospun polyester-urethane scaffold preserves mechanical properties and exhibits strain stiffening during in situ tissue ingrowth and degradation

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