Tissue leptin and plasma insulin are associated with lipoprotein lipase activity in severely obese patients

Ramis, Joana Maria; Bibiloni, Bartolomé; Moreiro, José; García-Sanz, José M.; Salinas, Ramón; Proenza, Ana M.; Lladó, Isabel

doi:10.1016/j.jnutbio.2004.12.009

Cited by 18 publications

(12 citation statements)

References 42 publications

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“…The lower portal vein leptin concentration is consistent with data obtained in vitro from isolated adipose tissue, showing that the expression of the ob gene, which produces leptin, and leptin secretion are lower in omental than subcutaneous fat (27,28).…”

Section: Discussionsupporting

confidence: 87%

Visceral Fat Adipokine Secretion Is Associated With Systemic Inflammation in Obese Humans

et al. 2007

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Although excess visceral fat is associated with noninfectious inflammation, it is not clear whether visceral fat is simply associated with or actually causes metabolic disease in humans. To evaluate the hypothesis that visceral fat promotes systemic inflammation by secreting inflammatory adipokines into the portal circulation that drains visceral fat, we determined adipokine arteriovenous concentration differences across visceral fat, by obtaining portal vein and radial artery blood samples, in 25 extremely obese subjects (mean ؎ SD BMI 54.7 ؎ 12.6 kg/m 2 ) during gastric bypass surgery at Barnes-Jewish Hospital in St. Louis, Missouri. Mean plasma interleukin (IL)-6 concentration was ϳ50% greater in the portal vein than in the radial artery in obese subjects (P ‫؍‬ 0.007). Portal vein IL-6 concentration correlated directly with systemic C-reactive protein concentrations (r ‫؍‬ 0.544, P ‫؍‬ 0.005). Mean plasma leptin concentration was ϳ20% lower in the portal vein than in the radial artery in obese subjects (P ‫؍‬ 0.0002). Plasma tumor necrosis factor-␣, resistin, macrophage chemoattractant protein-1, and adiponectin concentrations were similar in the portal vein and radial artery in obese subjects. These data suggest that visceral fat is an important site for IL-6 secretion and provide a potential mechanistic link between visceral fat and systemic inflammation in people with abdominal obesity.

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Section: Discussionsupporting

confidence: 87%

Visceral Fat Adipokine Secretion Is Associated With Systemic Inflammation in Obese Humans

et al. 2007

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“…The markedly increased basal rate of lipolysis in both fat depots from men compared to women correlated with the men's larger fat cells, in accordance with previously reported results [28,32,33], and also with a higher lipoprotein lipase activity [34]. Moreover, the lipolytic capacity and the effect of noradrenaline on lipolysis in fat depots of obese subjects were also influenced by gender.…”

Section: Gender Differences In the Lipolytic Capacity Of Adipocytes Fsupporting

confidence: 91%

Depot- and Gender-related Differences in the Lipolytic Pathway of Adipose Tissue from Severely Obese Patients

Ramis

Salinas

García-Sanz

et al. 2006

Cell Physiol Biochem

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The present study was performed to analyze in detail gender- and site-related alterations in the adrenergic signal transduction pathway of lipolysis in fat cells isolated from subcutaneous abdominal and visceral fat depots from severely obese patients. The study group consisted of 30 morbidly obese subjects (9 men and 21 women) aged 41.1±1.9 years, with a body mass index (BMI) of 54.7±1.7 kg/m2, who had undergone abdominal surgery. Protein levels of hormone-sensitive lipase (HSL) and adrenergic receptors (AR), as well as HSL activity and the lipolytic response to adrenergic agents were analyzed. Both fat depots had similar basal lipolysis, but the capacity of catecholamines to activate lipolysis was greater in visceral fat, both at AR and postreceptor levels. Basal lipolysis and lipolytic activity induced by dibutyryl cyclic AMP were higher in men than in women. However, the visceral depot of women showed a higher maximal stimulation by noradrenaline than that of men, in accordance with higher ß1- and ß3-AR protein levels. In conclusion, the main gender-related differences were located in the visceral depot, with women exhibiting a higher sensitivity to catecholamines associated with an increased provision of ß-AR, while men showed an enhanced lipolytic capacity at the postreceptor level.

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“…The profiles of gene expression of subcutaneous abdominal fat and of the omentum are different, including the expression of some proteins, LEP among them [32]. Studies conducted on samples of abdominal subcutaneous fat and omentum have shown that the gene expression of LEP is higher in the abdominal subcutaneous fat, a result observed both in men and in women and in obese and normal weight individuals [33]–[37]. Since we did not detect a significant difference in LEP expression in the omentum of the obese group compared to control, we may assume that this was due to the fact that the highest production of LEP occurs in subcutaneous.…”

Section: Discussionmentioning

confidence: 99%

The Expression of LEP, LEPR, IGF1 and IL10 in Obesity and the Relationship with microRNAs

et al. 2014

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Obesity is a multifactorial disease, with epigenetic alterations. Have been described modifications in the expression of some microRNAs, and some proteins related to obesity. The objective was to determine and correlate, in obese patients, the gene expression of LEP, LEPR, IGF1, IL10 and of miR-27a, miR-27b, miR-143 and miR-145. RNA was extracted from biopsies of subcutaneous fat, liver and visceral fat of 15 obese subjects submitted to bariatric surgery and of 15 non-obese subjects submitted to cholecystectomy for cDNA synthesis and for RT-PCR. The microRNAs were chosen using the TargetScan software. An increased expression of LEP and IGF1 was detected in the subcutaneous fat of the obese group compared to control, while the expression of IGF1 was higher in the control group than in the obese one. MiRNA-27a had a higher expression in the omentum of the obese patients and there was also a correlation in the expression of miRNA-145 and LEPR in the omentum of this group.

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Tissue leptin and plasma insulin are associated with lipoprotein lipase activity in severely obese patients

Cited by 18 publications

References 42 publications

Visceral Fat Adipokine Secretion Is Associated With Systemic Inflammation in Obese Humans

Visceral Fat Adipokine Secretion Is Associated With Systemic Inflammation in Obese Humans

Depot- and Gender-related Differences in the Lipolytic Pathway of Adipose Tissue from Severely Obese Patients

The Expression of LEP, LEPR, IGF1 and IL10 in Obesity and the Relationship with microRNAs

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