Tissue levels of tumor necrosis factor-alpha correlates with grade of inflammation in untreated ulcerative colitis

Olsen, Trine; Goll, Rasmus; Cui, Guanglin; Husebekk, Anne; Vonen, Barthold; Birketvedt, Grethe Støa; Florholmen, Jon

doi:10.1080/00365520701409035

Cited by 132 publications

(103 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…36,37 Total RNA was extracted from biopsies according to the Trizol method (Invitrogen, Paisley, UK) and quantified at 260 nm in a U-1500 UV/Vis spectrophotometer (Hitachi Instruments Inc, San Jose, CA). RNA integrity was assessed in an Agilent 2100 Bioanalyzer on RNA 6000 Nano chips (Agilent Technology, Bö blingen, Germany) according to the manufacturer's instructions and all samples included were found to be RIN Ն8 (RNA integrity number) on a scale from 0 to 10.…”

Section: Quantitative Pcr Analysismentioning

confidence: 99%

“…The stability of ␤-actin as a housekeeping gene in the present context has been verified earlier. 36 Cytokine transcript levels relative to those of ␤-actin were analyzed according to the comparative C T -method and expressed as 2 Ϫ⌬CT ϫ 10E4. 39 The PCR efficiencies were close to 2 for both the IL-33 and the ␤-actin cDNA using serial dilutions of the templates.…”

Section: Quantitative Pcr Analysismentioning

confidence: 99%

See 1 more Smart Citation

Inflammatory Bowel Disease-Associated Interleukin-33 Is Preferentially Expressed in Ulceration-Associated Myofibroblasts

Sponheim

Pollheimer

Olsen

et al. 2010

The American Journal of Pathology

Self Cite

134

127

View full text Add to dashboard Cite

Interleukin-33 (IL-33) is a novel member of the interleukin-1 family that induces mucosal pathology in vivo and may drive fibrosis development and angiogenesis. To address its potential role in inflammatory bowel disease, we explored its tissue expression in biopsy specimens from untreated ulcerative colitis patients, observing a 2.6-fold up-regulation of IL-33 mRNA levels, compared to controls. Immunohistochemical analyses of surgical specimens showed that a prominent source of IL-33 in ulcerative colitis lesions were ulceration-associated myofibroblasts that co-expressed the fibroblast marker heat shock protein 47, platelet-derived growth factor receptor (PDGFR)␤, and, in part, the myofibroblast marker ␣-smooth muscle actin (SMA).

show abstract

Section: Quantitative Pcr Analysismentioning

confidence: 99%

Section: Quantitative Pcr Analysismentioning

confidence: 99%

Inflammatory Bowel Disease-Associated Interleukin-33 Is Preferentially Expressed in Ulceration-Associated Myofibroblasts

Sponheim

Pollheimer

Olsen

et al. 2010

The American Journal of Pathology

Self Cite

134

127

View full text Add to dashboard Cite

show abstract

“…Some reports have found positive correlations with the degree of disease activity in UC (Akazawa et al, 2002;Olsen et al, 2007) and CD (Olsen et al, 2007) but not in all for CD (Akazawa et al, 2002) and UC (Dionne et al, 1997). As far as we know no studies do exist on mucosal expressions of TNF-α and the prediction of the clinical course, whereas there are only few reports on the predictive value of mucosal TNF-α concentrations and the response to therapy.…”

Section: Proinflammatory Cytokines 421 Tnf-αmentioning

confidence: 99%

“…The reason for this difference between the two diseases is not known. Both CD patients and UC patients have increased levels of cytokines belonging to T-helper-1 response (Olsen et al, 2007;Vermeire et al, 2006). Santos et al investigated 957 subjects and found strong association between CRP and central abdominal obesity (Santos et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Clinical, Biological, and Laboratory Parameters as Predictors of Severity of Clinical Outcome and Response to Anti–TNF–Alpha Treatment in Ulcerative Colitis

Olsen¹,

Florholmen²

2011

Ulcerative Colitis - Treatments, Special Populations and the Future

Self Cite

View full text Add to dashboard Cite

“…Dendritic cells (DCs) in UC show enhanced expression of TLR4, which is associated with microbial recognition, and these activated DCs induce production of proinflammatory cytokines (4). Intestinal tissue level of TNF-· is up-regulated in UC and correlates to the grade of inflammation (5). Colorectal cancer is one of the most serious complications of UC (6).…”

Section: Introductionmentioning

confidence: 99%

Vitamin D receptor expression is associated with colon cancer in ulcerative colitis

Wada

Tanaka²,

Maeda³

et al. 2009

Oncol Rep

View full text Add to dashboard Cite

Abstract. Since the 1970s, it has been well known that longstanding ulcerative colitis (UC) disposes to the development of colorectal adenocarcinoma (CRC). To date, CRC associated with UC is thought to arise along a pathway of dysplasia, however, primary factors for developing of UC-related dysplasia and cancer are unclear. Vitamin D, which works through binding the vitamin D receptor (VDR) has an important role in cancer progression and immune response. In this study, we investigated the impact of VDR expression on UC as well as colon cancer. We examined retrospectively the expression of VDR in extraction specimens of UC (n=124) patients by immunohistochemistry. We counted VDR positive cells in at least 10 fields in each case to evaluate the frequency of VDR positive cells in ductal epithelium. In addition, effect of VDR expression on inflammation was analyzed. On a normal mucosa, the expression of VDR was recognized in 58.8% of ductal cells. In UC patient, the expression of VDR was considerably decreased compared to normal mucosa, VDR positive rate was only 3.4±9.0%. Importantly, dysplasia and UC-CRC patients showed lower rate of VDR expression compared to non-colon cancer patients, whose expression rates were 0.6±1.3% and 3.8±10%, respectively. Moreover, long-term UC patients (more than ten years) who were at high-risk of developing CRC showed significantly lower VDR expression than shortterm patients. We did not detect direct association of VDR expression with inflammation and clinical stage of UC. These findings suggested that correlation seems to exist between the level of VDR expression and carcinogenesis in UC. VDR could be a possible marker to detect dysplasia and cancer in ulcerative colitis.

show abstract

Tissue levels of tumor necrosis factor-alpha correlates with grade of inflammation in untreated ulcerative colitis

Abstract: TNF-alpha is highly expressed in UC and correlates to the grade of inflammation. The sources of TNF-alpha were observed both in CD3+ lymphocytes and in macrophages. Cytokine expression (mRNA) profiles seem to be similar in patients with moderate to severe UC and CD.

Cited by 132 publications

References 40 publications

Inflammatory Bowel Disease-Associated Interleukin-33 Is Preferentially Expressed in Ulceration-Associated Myofibroblasts

Inflammatory Bowel Disease-Associated Interleukin-33 Is Preferentially Expressed in Ulceration-Associated Myofibroblasts

Clinical, Biological, and Laboratory Parameters as Predictors of Severity of Clinical Outcome and Response to Anti–TNF–Alpha Treatment in Ulcerative Colitis

Vitamin D receptor expression is associated with colon cancer in ulcerative colitis

Contact Info

Product

Resources

About