Tissue metabolomic profiling to reveal the therapeutic mechanism of reduning injection on LPS-induced acute lung injury rats

Abstract

A metabolomic strategy has been developed to investigate the therapeutic mechanistic insights of RDN on the ALI.

Targeted and untargeted metabolomic analysis of Procambarus clarkii exposed to a “chemical cocktail” of heavy metals and diclofenac

“…Untargeted metabolomic fingerprinting 2.6.1. UPLC-ESI-QTOF-MS analysis, data processing and metabolite identification UPLC-QTOF-MS experiments were performed using an Agilent 6550 iFunnel Q-TOF LC/MS System (Agilent Technologies, Tokyo, Japan) coupled to an Agilent 1290 Series LC pump with a reversed phase chromatography using the methodology described by Xiong et al (2018). Briefly, 10 μL of the extract were placed into an autosampler maintained at 4 • C and injected into an Acquity® HSS T3 column (100 mm × 2.1 mm, 1.8 μm) at 40 • C. The mobile phase flow was set at 0.2 mL min − 1 with solvent A (0.1% (v/v) formic acid in water) and solvent B (0.1% (v/v) formic acid in acetonitrile).…”

Systems pharmacology reveals the mechanism of activity of Physalis alkekengi L. var. franchetii against lipopolysaccharide‐induced acute lung injury

“…Compared with the control group, the serum levels of betaine, taurine, carnitine and glutamine in the LPS group were significantly reduced, further confirming the occurrence of oxidative stress injury. According to many studies, betaine and taurine not only act as antioxidant agents, but also as organic regulatory osmolytes to protect the cell from oxidative damage, and maintain the structural and functional integrity of the membranes . Carnitine is also a widely known antioxidant and protector against apoptosis.…”

Assessing potential liver injury induced by Polygonum multiflorum using potential biomarkers via targeted sphingolipidomics

“…Principal component analysis (PCA) was used to visually discriminate groups (Xiong et al. 2018 ). Data in each group were generated using a normalized mean-centred unit-variance scale.…”