Tissue Microarrays for Translational Research

Simon, Ronald; Mirlacher, Martina

doi:10.1007/978-3-642-28203-4_9

Cited by 1 publication

(1 citation statement)

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“…A tissue core is transferred from the “donor” paraffin block to the “recipient” block, which may contain up to 1000 cores. 344 This technology is used not only for protein detection but also for gene expression. † Tissue microarray techniques include multitumor arrays (samples from tumors of multiple histological types), progression arrays (samples of normal tissue and different stages of tumor progression within a given organ), prevalence arrays (tumor samples from 1 or several entities without extensive clinicopathologic information to test biomarkers with possible therapeutic implications), prognosis/outcome-based arrays (samples with clinical follow-up data to evaluate prognostic or predictive biomarkers), cell line arrays (normal or cancer cell lines grown in culture to determine the specificity of antibodies targeting a protein), heterogeneity/random tissue/tumor arrays (tumor and nontumor tissues from different sites for monitoring the intratumoral heterogeneity of molecular markers), and cryomicroarrays (frozen samples that might be more suitable than formalin-fixed tissues for RNA detection).…”

Section: Preanalytical Phase Of Ihcmentioning

confidence: 99%

When Tissue Antigens and Antibodies Get Along

2013

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Once focused mainly on the characterization of neoplasms, immunohistochemistry (IHC) today is used in the investigation of a broad range of disease processes with applications in diagnosis, prognostication, therapeutic decisions to tailor treatment to an individual patient, and investigations into the pathogenesis of disease. This review addresses the technical aspects of immunohistochemistry (and, to a lesser extent, immunocytochemistry) with attention to the antigen-antibody reaction, optimal fixation techniques, tissue processing considerations, antigen retrieval methods, detection systems, selection and use of an autostainer, standardization and validation of IHC tests, preparation of proper tissue and reagent controls, tissue microarrays and other high-throughput systems, quality assurance/quality control measures, interpretation of the IHC reaction, and reporting of results. It is now more important than ever, with these sophisticated applications, to standardize the entire IHC process from tissue collection through interpretation and reporting to minimize variability among laboratories and to facilitate quantification and interlaboratory comparison of IHC results.

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Section: Preanalytical Phase Of Ihcmentioning

confidence: 99%