Tissue Permeability and the Spreading Factors in Infection

“…In the present study cfb gene was discovered at 66.66%, which is similar to Shome et al, (2012) reported 85.7% prevalence of cfb gene among S. agalactiae isolates. Still the significance of CAMP factor in pathogenicity of S. agalactiae is not properly know, hence it is not putative virulence factor (Lasagno et al, 2011).The hylB gene codes for hyaluronate lyase which help to break hyaluronic acid, N-acetylglucosamine and glucuronic acid (components of extracellular matrix) It also known as spreading factor which helps to the host tissue invasive (Duran-Reynals, 1942). In present study we found 83.33% isolates were positive for hylB, these results are in accordance with those of Gunther et al, (1996), according to their 72% of the GBS were hylB positive whereas Dmitriev et al, (2002) reported hylB gene in all the isolates studied.…”

Section: Detection Of Virulence Associated Genesmentioning

confidence: 99%

Isolation, Antibiogram and Molecular Characterization of Group B Streptococci Isolates from Bovine Mastitis

Singh

Arora

Rai

et al. 2021

IJAR

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Background: Group B streptococcus (GBS) or Streptococcus agalactiae is an important pathogen associated with bovine mastitis. The organism is also of public health consequences and may cause variety of infections ranging from neonatal sepsis, pneumonia and meningitis to localized infections and urinary tract infection or arthritisin adult humans. Widespread use of antibiotics in veterinary medicine has led to development of resistance among the pathogens. So there is need for surveillance of antimicrobial resistance to ensure effective treatment. Methods: Milk samples collected from mastitis affected animals were processed for isolation of Streptococcus agalactiae. The isolates were tested for antimicrobial susceptibility. Molecular characterisation was carried out by PCR to study the occurrence of resistance marker genes and virulence marker genes. RAPD was carried out to study genetic diversity among the isolates. Result: Six isolates of S. agalactiae were obtained from 182 milk samples. Highest resistance was observed against co-trimoxazole and tetracycline followed by ampicillin. tetM gene and tetO genes could be amplified in four and three isolates, respectively. None of the isolates showed amplification for ermA, ermB, mefA and mefE genes. Three isolates were positive for the five virulence genes tested (glnA, cfb, hylB, scaA and cyl). RAPD analysis demonstrated great intraspecific genetic diversity among the streptococcal isolates.

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“…Comprising distinct layers, including the epidermis, dermis, and hypodermis [1,2] , it provides multifunctional protection ranging from thermal regulation to infection prevention [3,4] . However, despite its sophisticated defense mechanisms, the skin remains vulnerable to various conditions [5][6][7] , among which parasitic infections are significant. This intricate interface between the host and the environment can be the site of various skin diseases, with cutaneous leishmaniasis emerging as a significant concern.…”

Section: Introductionmentioning

confidence: 99%

Cutaneous Leishmaniasis: Physiopathology, Molecular Diagnostic, and Therapeutic Approaches

Balahbib,

Hmamouch,

El Allam

et al. 2023

Prog Micobes Mol Biol

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This in-depth study explores various aspects of cutaneous leishmaniasis, shedding light on the physiopathology of the infection, advances in molecular diagnostic techniques, and therapeutic approaches currently in development. Our investigation seamlessly integrates fundamental and clinical research data to provide a comprehensive perspective on this debilitating disease. Cutaneous leishmaniasis, primarily manifested through skin lesions, poses a significant diagnostic challenge. We delve into molecular diagnostic methods, especially PCR, emphasizing their crucial role in accurately detecting the infection. Simultaneously, we examine the implications of the physiopathology of cutaneous leishmaniasis, unveiling the complex mechanisms underlying this disease. In terms of treatment, we scrutinize current therapeutic approaches, highlighting limitations associated with using antimonials. Our study also explores alternative solutions, investigating the potential benefits of flavonoids and other natural compounds, thus offering innovative therapeutic perspectives. In conclusion, this research aims to enhance the understanding of cutaneous leishmaniasis from a diagnostic and therapeutic standpoint. This review is an essential resource for healthcare professionals and researchers, laying the groundwork for an integrated and holistic approach to better comprehend and treat this complex disease.

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Tissue Permeability and the Spreading Factors in Infection

Cited by 209 publications

References 88 publications

Intercellular Cement and Capillary Permeability

Intercellular Cement and Capillary Permeability

Isolation, Antibiogram and Molecular Characterization of Group B Streptococci Isolates from Bovine Mastitis

Cutaneous Leishmaniasis: Physiopathology, Molecular Diagnostic, and Therapeutic Approaches

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