1995
DOI: 10.1016/0169-328x(94)00219-5
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Tissue pH as an indicator of mRNA preservation in human post-mortem brain

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Cited by 312 publications
(191 citation statements)
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“…Thus, it cannot be assumed that because one particular protein, mRNA, etc is stable, all are stable during a certain PMI. In addition to PMI and storage temperature, other factors such as agonal state at death (Bowen et al, 1976;Butterworth et al, 1983;Harrison et al, 1991;Lewis and Akil, 1997;Perry et al, 1977aPerry et al, , 1982Spokes, 1979;Yates et al, 1990), pH (generally related to agonal state) (Barton et al, 1993;Eastwood and Harrison, 2000;Harrison et al, 1995;Johnston et al, 1997;Kingsbury et al, 1995), freezing time (Roytta et al, 1980), and time or season of death (Bucht et al, 1981;Carlsson et al, 1980; Perry et al, 1977a-d), among many others, have been found to influence the degradationFor levelFof biological products in postmortem brain specimens. Thus, it remains critical to ascertain the post-mortem stability, and the stability under other conditions, of the biological product.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, it cannot be assumed that because one particular protein, mRNA, etc is stable, all are stable during a certain PMI. In addition to PMI and storage temperature, other factors such as agonal state at death (Bowen et al, 1976;Butterworth et al, 1983;Harrison et al, 1991;Lewis and Akil, 1997;Perry et al, 1977aPerry et al, , 1982Spokes, 1979;Yates et al, 1990), pH (generally related to agonal state) (Barton et al, 1993;Eastwood and Harrison, 2000;Harrison et al, 1995;Johnston et al, 1997;Kingsbury et al, 1995), freezing time (Roytta et al, 1980), and time or season of death (Bucht et al, 1981;Carlsson et al, 1980; Perry et al, 1977a-d), among many others, have been found to influence the degradationFor levelFof biological products in postmortem brain specimens. Thus, it remains critical to ascertain the post-mortem stability, and the stability under other conditions, of the biological product.…”
Section: Discussionmentioning
confidence: 99%
“…57,60,61 Correlation analysis showed a significant association between PSD-93 and age in ACC (r = 0.38, Pvalue = 0.000009). Expression of PSD-93, SAP102 and NF-L were significantly associated with PMI in DLPFC (r = 0.32, P = 0.0008; r = 0.45, P = 0.000001; r = 0.38, P = 0.00008) and NF-L in ACC (r = 0.30; P = 0.0003).…”
Section: Resultsmentioning
confidence: 97%
“…Tissue pH has been described as an important factor for mRNA and possibly protein stability. 25,26 Where reduced pH is associated with reduced or absent mRNA. Therefore, the altered abundance of DDAH-1 and DRP2 may be attributed to differences in pH.…”
Section: Two-dimensional Gel Electrophoresis Comparison Of Normal Andmentioning
confidence: 99%
“…Similarly, the level of some proteins have changed with prolonged PMI, showing altered levels or truncation on 2DE, with most changes appearing at a PMI of 24 h or later. 26,27 Therefore, the level of Acetyl CoA acetyltransferase, a protein involved in glucose metabolism in times of oxidative stress, may not be due to the disease itself.…”
Section: Two-dimensional Gel Electrophoresis Comparison Of Normal Andmentioning
confidence: 99%