Tissue Polypeptide Antigen and Keratins in Cervical Neoplasia

He, Stegner; Kühler, C; Löning, Thomas

doi:10.1097/00004347-198603000-00003

Cited by 10 publications

(4 citation statements)

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“…The degree of keratinization (differentiation) is a pathological feature of cervical tumors (39,40), especially described as a frequent incidence in HPV-16 positive cervical carcinomas (41). In several of the malignant cervical tumors, which were analyzed in this study, we found areas of various differentiation grades exist next to each other, as described by others.…”

Section: E7/prb Interaction In Hsil and Carcinomassupporting

confidence: 79%

High level HPV‐16 E7 oncoprotein expression correlates with reduced pRb‐levels in cervical biopsies

et al. 2004

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High-risk human papillomaviruses (HPVs) are major etiological agents of cervical cancer. Despite excellent epidemiological evidence for a direct role of HPV-16 in cervical carcinogenesis, molecular pathways underlying carcinogenesis in vivo remain obscure. The E7 gene is required for immortalization and maintenance of the transformed phenotype in vitro; however, little is known about its role for tumorigenesis in vivo. The E7 gene codes for an unstable protein the abundance of which in cervical biopsies is unknown. We show here that E7 protein levels strongly increase during cervical carcinogenesis, underlining its fundamental role in cervical cancer. The E7 protein was found predominantly in the nucleus and to a minor extent in the cytoplasm in the cervical cancer cell line Ca Ski in vitro and in invasive cervical carcinoma in situ, suggesting that nuclear resident E7 plays a major role in cervical carcinogenesis in humans. The retinoblastoma protein (pRb) is a major E7-target in vitro. We show here that pRb expression is initially upregulated in LSIL and disappears in later stages concomitant with increased E7 levels, suggesting that E7-driven degradation of pRb is involved in cervical tumorigenesis in humans.

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Section: E7/prb Interaction In Hsil and Carcinomassupporting

confidence: 79%

High level HPV‐16 E7 oncoprotein expression correlates with reduced pRb‐levels in cervical biopsies

et al. 2004

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“…Further studies utilising SIHA supernatant generated in serum-free medium as the coated antigen in the ELISA assay also demonstrated the presence of the shedding antigen in the culture supernatant (Figure 4b aOnly the basal cells were positive. 18 and 19 that is also located in the basal cells of the normal squamous epithelium; however it has rarely been found in keratinised cervical SCC and is absence in the normal endocervical columnar cells (Stegner et al, 1986). MAb 17.13 reacts with a basal cell antigen, but is not present in adenocarcinomas (Randen et al, 1987).…”

Section: Resultsmentioning

confidence: 99%

Production and characterisation of a monoclonal antibody (Cx-99) against cervical carcinoma

Yuan

Tsai²,

Hsu³

et al. 1992

Br J Cancer

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Summary An IgGI monoclonal antibody (MAb Cx-99) has been established which recognises a surface antigen on epithelial cells, but not on fibroblastic or hematopoietic cells. Immunohistochemical studies showed that this antigen was present in all 37 squamous cell carcinomas (SCC) including 33 cervical SCC, and 30 of the 32 adenocarcinomas examined; most of the 33 cervical SCC were stained extensively. It was also detected in the culture medium of cervical cancer cell lines. In the normal cervix, this antigen was restricted to the undifferentiated basal cells. This observation suggests that the widespread expression of the antigen was triggered by oncogenesis. The MAb Cx-99 recognised an epitope on an asialyted glycoprotein which has an apparent molecular weight of 37 kilodaltons (kD) (and 2 minor proteins at 18 and 27 kD) and an isoelectric point (pI) of 5.3. It may have potential for studies on differentiation and oncogenesis and for diagnostic applications.

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“…Several investigators have examined keratin expression in cervical epithelium 104–111. The cervix appears to have lower molecular weight keratins (39 kD, 43 kD, and 58 kD) than, for example, the aerodigestive tract epithelium.…”

Section: Differentiation Markersmentioning

confidence: 99%

Surrogate endpoint biomarkers and their modulation in cervical chemoprevention trials

Follen

Schottenfeld

2001

Cancer

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BACKGROUND. Surrogate endpoint biomarkers (SEBs) are used as intermediate indicators of a reduction in cancer incidence in chemoprevention studies. SEBs should be expressed differentially in normal and high risk tissue; appear at a well defined stage of carcinogenesis; be studied with reasonable sensitivity, specificity, and accuracy; and be modulated in chemoprevention trials. The concept of SEBs may be useful in the trials of many new therapies. METHODS.The current review includes a comprehensive review of the literature.Many SEBs have been the subject of intense study and include quantitative histopathology and cytology, proliferation markers, regulation markers, differentiation markers, general genomic instability markers, and tissue maintenance markers. Because of the critical biologic and epidemiologic role of the human papillomavirus (HPV) in cervical carcinogenesis, the relation between these markers and HPV should be considered. In addition, biomarkers of HPV infection and its regression should be sought. RESULTS. Several chemoprevention trials have beenpublished that have included the use of SEBs. The biomarkers that appear most promising in these clinical trials can be measured quantitatively and reproducibly: quantitative histology and cytology, proliferating cell nuclear antigen (PCNA), MIB-1, MPM-2, HPV viral load, epidermal growth factor receptor, polyamines, and ploidy. The markers that have been demonstrated to be modulated in chemoprevention trials in the literature are quantitative histology and cytology, PCNA, MPM-2, HPV viral load, and polyamines. CONCLUSIONS.The surrogate endpoint biomarkers of most interest in future research should correlate well with HPV infection, be modulated by several therapeutic agents, and have limited variability and ease in measurement. Cancer 2001;

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Tissue Polypeptide Antigen and Keratins in Cervical Neoplasia

Cited by 10 publications

References 0 publications

High level HPV‐16 E7 oncoprotein expression correlates with reduced pRb‐levels in cervical biopsies

High level HPV‐16 E7 oncoprotein expression correlates with reduced pRb‐levels in cervical biopsies

Production and characterisation of a monoclonal antibody (Cx-99) against cervical carcinoma

Surrogate endpoint biomarkers and their modulation in cervical chemoprevention trials

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