Tissue processing

Wolfe, Dee

doi:10.1016/b978-0-7020-6864-5.00006-2

Cited by 18 publications

(7 citation statements)

References 4 publications

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“…This is primarily attributed to the removal of substantial quantities of hydrogen and oxygen elements from the sample, leading to a densification of the organic elements proportionate to their hydration level. This densification contributes to subtle distinctions between elements with varying degrees of hydration 61 , 62 .…”

Section: Discussionmentioning

confidence: 99%

A high-throughput 3D X-ray histology facility for biomedical research and preclinical applications

Katsamenis,

Basford,

Robinson

et al. 2023

Wellcome Open Res

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Background The University of Southampton, in collaboration with the University Hospital Southampton (UHS) NHS Foundation Trust and industrial partners, has been at the forefront of developing three-dimensional (3D) imaging workflows using X-ray microfocus computed tomography (μCT) -based technology. This article presents the outcomes of these endeavours and highlights the distinctive characteristics of a μCT facility tailored explicitly for 3D X-ray Histology, with a primary focus on applications in biomedical research and preclinical and clinical studies. Methods The UHS houses a unique 3D X-ray Histology (XRH) facility, offering a range of services to national and international clients. The facility employs specialised μCT equipment explicitly designed for histology applications, allowing whole-block XRH imaging of formalin-fixed and paraffin-embedded tissue specimens. It also enables correlative imaging by combining μCT imaging with other microscopy techniques, such as immunohistochemistry (IHC) and serial block-face scanning electron microscopy, as well as data visualisation, image quantification, and bespoke analysis. Results Over the past seven years, the XRH facility has successfully completed over 120 projects in collaboration with researchers from 60 affiliations, resulting in numerous published manuscripts and conference proceedings. The facility has streamlined the μCT imaging process, improving productivity and enabling efficient acquisition of 3D datasets. Discussion & Conclusions The 3D X-ray Histology (XRH) facility at UHS is a pioneering platform in the field of histology and biomedical imaging. To the best of our knowledge, it stands out as the world's first dedicated XRH facility, encompassing every aspect of the imaging process, from user support to data generation, analysis, training, archiving, and metadata generation. This article serves as a comprehensive guide for establishing similar XRH facilities, covering key aspects of facility setup and operation. Researchers and institutions interested in developing state-of-the-art histology and imaging facilities can utilise this resource to explore new frontiers in their research and discoveries.

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Section: Discussionmentioning

confidence: 99%

A high-throughput 3D X-ray histology facility for biomedical research and preclinical applications

Katsamenis,

Basford,

Robinson

et al. 2023

Wellcome Open Res

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“…Established protocols were used for histological processing and RNA extraction (Alsamahi et al, 2022; Friedlander et al, 2018; Wolfe, 2019). For IHC, T2D ( n = 10) and non‐T2D ( n = 10) teeth were sectioned horizontally below the cemento‐enamel junction (CEJ) and the coronal portion immediately placed in 10% neutral buffered formalin for 24 h. Specimens were decalcified in 10% ethylenediaminetetraacetic acid solution (EDTA) (pH 7.4), trimmed and placed in phosphate‐buffered saline for 24 h to remove residual EDTA.…”

Section: Methodsmentioning

confidence: 99%

Effects of glycation end‐products on the dental pulp in patients with type 2 diabetes

Alsamahi,

Milne,

Mohd Hussaini

et al. 2023

Int Endodontic J

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AimThis ex vivo study aimed to compare protein expression of advanced glycation end‐products (AGE) and receptor (RAGE), and the levels of selected genes associated with inflammation and collagen within dental pulp tissue from patients with type 2 (T2D) diabetes and non‐T2D.MethodologyNoncarious extracted permanent molar teeth from patients with well‐controlled T2D (n = 19) and non‐T2D (controls) (n = 19) were collected and compared. The coronal pulp was examined using immunohistochemistry (IHC) (n = 10 per group) for anti‐AGE and anti‐RAGE. Quantitative PCR (n = 9 per group) was used to analyse the gene expression levels of NFKB, S100A12 and COLIA1. Data analyses were performed between the groups using GraphPad Prism using Pearson correlation, Shapiro–Wilk and Mann–Whitney U‐tests, and multiple regression using SPSS.ResultsAGEs were distributed diffusely throughout the pulp extracellular matrix associated with collagen fibres and were present on several cell types. RAGE was expressed at the pulp–dentine interface and was observed on odontoblasts, immune cells, endothelial cells and fibroblasts. Semi‐quantitative analysis of IHC samples showed significantly increased expression of AGE (p < .0001) and RAGE (p = .02) in T2D samples compared with controls. The expression of NFKB (p < .0001), S100A12 (p < .0001) and COLIA1 (p = .01) genes were significantly higher in the T2D pulp, and multivariate logistic regression analysis showed that these findings were not affected by age.ConclusionT2D may exert a similar glycation response in the dental pulp to other body sites. This could occur through activation of NF‐κB pathways with a concomitant increase in genes associated with inflammation and collagen.

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“…Each fish was placed in right lateral recumbency, the left lateral body wall and operculum were removed, and the entire body was placed into 10% neutral buffered formalin. The bodies were decalcified in Kristensen's solution, serially sectioned transversely, and placed into tissue cassettes for histologic processing according to standard methods (Wolfe, 2019). Formalin‐fixed, paraffin embedded tissues were sectioned at 4 μm and stained with either haematoxylin and eosin, Masson's trichrome, modified Brown‐Hopps (Gram), Giemsa, or Grocotts's Methamine Silver (GMS) stains.…”

Section: Figurementioning

confidence: 99%

Gonadal neoplasia in Chinese gold barbs (Barbodes semifasciolatus) from a home aquarium

Stilwell,

Camus,

Stilwell

2023

Journal of Fish Diseases

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Tissue processing

Cited by 18 publications

References 4 publications

A high-throughput 3D X-ray histology facility for biomedical research and preclinical applications

A high-throughput 3D X-ray histology facility for biomedical research and preclinical applications

Effects of glycation end‐products on the dental pulp in patients with type 2 diabetes

Gonadal neoplasia in Chinese gold barbs (Barbodes semifasciolatus) from a home aquarium

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