Tissue reaction initiated by different sealers

Bernåth, M; Szabó, János

doi:10.1046/j.1365-2591.2003.00662.x

Cited by 96 publications

(85 citation statements)

References 23 publications

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“…Although sealers should be confined within the root canal, their inadvertent extrusion beyond the apical foramen into the periradicular tissues may occur 3) . Thus toxic components of sealers, particularly in cases of open apex due to iatrogenic over-instrumentation, pathologic root resorption, or non-vital teeth with immature roots, would damage both the periodontal ligament and alveolar bone 4) , adversely affecting the treatment outcome. Therefore, sealers should have good biocompatibility and should be well tolerated by the periradicular tissues 5) .…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Kim

Shin

2014

Dent. Mater. J.

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The objective of this study was to assess the cytotoxicity of EndoSeal, a novel mineral trioxide aggregate-based root canal sealer in comparison with two commonly used sealers, AH Plus and Sealapex. For cytotoxicity assay, MG-63 cells and human gingival fibroblasts were incubated in culture medium containing eluates of each sealer at 1, 3, and 7 days. Cell metabolism was evaluated by the WST-1 assay. For cell adhesion assay, disc specimens were fabricated from EndoSeal and AH Plus. MG-63 cells and human gingival fibroblasts were seeded on the discs, and after 1 day and 7 days of incubation, cell morphology and cell adhesion were examined by SEM. The order of cytotoxicity of root canal sealers was as follows: EndoSeal < AH Plus < Sealapex. Both types of cells seeded on the EndoSeal specimens were much larger, flat with rough margins compared to those on the AH Plus specimens. These results suggest that EndoSeal has a satisfactory cytocompatibility.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Kim

Shin

2014

Dent. Mater. J.

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“…Average thickness of the fibrous capsules of each specimen was evaluated in 10 separate areas. Fibrous capsules were considered to be thin when thickness was <150 μm and thick at >150 μm 11,13) . Evaluation of new bone formation around the implanted materials was carried out as follows: Grade 0, no bone formation; Grade I, Slight, presence of bony islets and coverage of less than 25% of the material surface with bone; Grade II, Moderate, coverage of at least 50% of the material surface with bone; Grade III, Extensive, complete coverage of the material surface with bone or the formation of an osseous bridge around the material 14) .…”

Section: Evaluation Parametersmentioning

confidence: 99%

Tissue reactions after subcutaneous and intraosseous implantation of iRoot SP, MTA and AH Plus

Zhang

Peng

2015

Dental Materials Journal

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The purpose of this study was to evaluate the reactions of rat subcutaneous connective tissue and bone tissue to iRoot SP, ProRoot MTA, and AH Plus. Specimens were implanted in the dorsal subcutaneous connective tissue and tibias of 36 Wistar rats. Tissue specimens were collected after rats were sacrificed at 7, 30, and 60 days after implantation. Histologic evaluations comprising inflammation severity and new bone formation were blindly made on hematoxylin and eosin-stained sections. In subcutaneous implant test, AH Plus group showed more infiltration of inflammatory cells than the other three groups at 30 days (p<0.05). In intraosseous implant test, number of inflammatory cells decreased in all groups at 7, 30, and 60 days after implantation with no statistically significant differences. Given the similar inflammatory reactions to iRoot SP and MTA, it was concluded that iRoot SP and MTA were biocompatible with the subcutaneous and intraosseous tissues of rats.

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“…Unfortunately, several studies have shown that most commercially available materials can cause mild to severe inflammatory changes in apical tissues, leading to bone destruction [21][22][23][24]. Endodontic sealants may also affect periradiculare tissues by direct contact in cases of overfilling or extracted components.…”

Section: Discussionmentioning

confidence: 99%

“…Endodontic sealants may also affect periradiculare tissues by direct contact in cases of overfilling or extracted components. They can be released into the surrounding tissues by the dentin tube and channels from the apical level [21][22][23][24].…”

Section: Discussionmentioning

confidence: 99%

Testing endodontic sealers on human umbilical vein endothelial cells

et al. 2013

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AbstractThe toxic potential of the endodontic sealers ingredients, especially the unreacted monomer, that can irritate the periapical tissue and interfere with the healing process, thus having a negative impact on the biocompatibility of the material. The aim of this study was to evaluate the influence of three experimental endodontic sealers on cells viability in vitro. Human umbilical vein endothelial cells (HUVEC) were used. The experiments were done with solid samples and extracts of sealers in artificial saliva and water. The experiments evaluated the cytotoxicity of the residual monomers that resulted from the tested composites. The decrease in cell viability was quantified by colorimetric measurement of formazan. The components of the sealers dissolved in artificial saliva and water were determined by high performance liquid chromatography (HPLC). The HUVEC are a novelty for testing the endodontic sealers biocompatibility, with certain advantages compared to other cell types used in the literature, e.g. HELA cells, fibroblasts. The data showed that cytotoxicity was directly linked with the unreacted monomer — 2-hydroxyethyl methacrylate (HEMA) present in these composites. Two of the three formulations had little or no cytotoxic effect, which makes them suitable for further testing in order to be used in endodontic treatment.

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Tissue reaction initiated by different sealers

Abstract: This study suggests that sealers with different chemical compositions initiate different histological reactions. It also emphasizes the importance of confining root filling to the canal system because all sealers initiate inflammatory reactions when they are present in the apical tissues

Cited by 96 publications

References 23 publications

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Tissue reactions after subcutaneous and intraosseous implantation of iRoot SP, MTA and AH Plus

Testing endodontic sealers on human umbilical vein endothelial cells

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