Tissue reactions of suture materials (polyglactine 910, chromed catgut and polydioxanone) on rat bladder wall and their role in bladder stone formation

Abstract

Suture materials are widely used in urological surgery especially in regions that are in contact with urine. In this study, we aimed to compare polyglactine 910, chromed catgut and polydioxanone sutures according to stone formation and inflammation, congestion and foreign body reaction that occur on bladder mucosa. Cystotomy procedure was performed, in three groups of Wistar female rats, with 4/0 polyglactine 910, 4/0 chromed catgut and 4/0 polydioxanone sutures. All groups were divided into two sub-groups with 4 and 8-week follow up periods. Rats were treated with 20 mg kg(-1) day(-1) Ofloxacin (i.p.) daily until the seventh post-operative day. Urinary pH, leucocyte esterase and nitrite levels were determined. All rats were killed at the end of the follow-up period and stone formation on sutures and degrees of tissue reactions (inflammation, congestion and foreign body reaction) on bladder mucosa were compared. Tissue reactions were evaluated by the same pathologist (S. K.). Chi-square and Student's t test were used in statistical analysis (p<0.05). There was no significant difference between the mean weights of the groups. Leucocyte esterase and nitrite were negative in urine analyses. There was no significant difference between urinary pH levels of the groups with 4 and 8 weeks follow-up (p>0.05). Although the difference between the degrees of congestion in groups was not statistically significant (p>0.05), there were statistically significant differences between the degrees of inflammation and foreign body reaction in groups. Although the duration of urinary contact of suture is the main factor in stone formation on suture material, tissue reaction on mucosa and the physical structure of suture also affect this formation. We observed lower degrees of inflammation and foreign body reaction with 4/0 polydioxanone and no stone formation. We believe that polydioxanone may be useful and reliable in urological surgery due to these properties.

Polyvinylidene Fluoride as a Suture Material: Evaluation of Comet Tail-Like Infiltrate and Foreign Body Granuloma

“…This might be due to the fact that it was the only braided type of suture evaluated, which could therefore have induced a more pronounced inflammation process. This also corresponds with other studies [24,25]. …”

Characterization of the deposition of collagen fibers and Lithogenic potential in bladder of rats submitted to a sugar cane biopolymer graft

“…The chosen control was polyglactin 910, a multifilamentar copolymer of biological source made of 90% glycolide and 10% L-lactide, nonantigenic, non-pyrogenic, widely described in the literature in order to compare the histological response in the bladder wall with the results of that new material (1,2). It is indicated for the use in all but cardiovascular and neurological tissues, and is absorbable with minimal inflammatory reaction (10).…”

“…Experimental and clinical studies research the best material with less immunological reaction, low cost and low inflammatory reaction, to reduce the risk of urinary stones and fistulae. In the urinary tract, several materials present a good response: polyglactin 910, polydioxanone, simple and chrome catgut, polyglicolic acid, all of them absorbable in variable times (1)(2)(3). As an advance Vol.…”

Tissue Response to Four Different Suture Materials Used to Repair Fascia Defect In Single Port Cholecystectomy Operations

“…In some experimental rat studies, the endurance caused by suture materials and tissue reaction were evaluated, and poliglecaprone and polydioxanone were found to be appropriate suture materials for wide-defect area repairs. In these studies, polyglactin 910 was found to create severe tissue reaction, and thus, it should be used on non-infected areas [14,15] .…”