Tissue remodeling: a mating-induced differentiation program for the Drosophilaoviduct

Kapelnikov, Anat; Rivlin, Patricia K.; Hoy, Ronald R.; Heifetz, Yael

doi:10.1186/1471-213x-8-114

Cited by 47 publications

(67 citation statements)

References 57 publications

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“…In accordance with these observations, our comparative analysis of genes regulated by both mating and 20E in the atrium identified a number of cytoskeleton-(Actin 5C, β-tubulin, adducin) and muscle-associated (myosins, Mlp84B) genes that may be involved in remodeling of this tissue. Atrial remodeling may also promote future oviposition; dramatic ultrastructural changes recorded in the oviduct of Drosophila females after mating ultimately induce the opening of the upper oviduct lumen, allowing egg release from the ovaries (49). Another plausible mechanism for the effects of 20E in egg-laying emerges from our analyses.…”

Section: Discussionmentioning

confidence: 82%

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Gabrieli

Kakani

Mitchell

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

112

174

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Significance Anopheles gambiae females are the principal vectors of malaria, a disease that kills more than 600,000 people every year. Current control methods using insecticides to kill mosquitoes are threatened by the spread of resistance in natural populations. A promising alternative control strategy is based on interfering with mosquito reproduction to reduce the number of malaria-transmitting females. Here we show that a male hormone transferred to the female during sex induces large changes in female behavior. These changes, defined as the postmating switch, include a physical incapacity for fertilization by additional males and the ability to lay mature eggs. Tampering with the function of this hormone generates unprecedented opportunities to reduce the reproductive success of Anopheles mosquitoes and impact malaria transmission.

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Section: Discussionmentioning

confidence: 82%

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Gabrieli

Kakani

Mitchell

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

112

174

View full text Add to dashboard Cite

show abstract

“…Additionally, recent reports by Kapelnikov et al (33,34) show that mating up-regulates genes and proteins with known roles in muscle and epithelial differentiation in the oviduct (33), and that structural changes occur in the oviduct postmating (34). Analogous changes may occur in the uterus postmating.…”

Section: Uterine Conformational Changes Are Important For Maximal Spermmentioning

confidence: 99%

“…An additional RNAi-generating line for Acp36DE was obtained from the Vienna Drosophila RNAi Center (construct ID: 37993) (38). Knockdowns were confirmed by Western blotting as in Ravi Ram et al (34).…”

Section: Generation Of Rnai Linesmentioning

confidence: 99%

Acp36DE is required for uterine conformational changes in mated Drosophila females

Avila

Wolfner

2009

Proc. Natl. Acad. Sci. U.S.A.

114

134

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In a multitude of animals with internal fertilization, including insects and mammals, sperm are stored within a female's reproductive tract after mating. Defects in the process of sperm storage drastically reduce reproductive success. In Drosophila males, ''Acp'' seminal proteins alter female postmating physiology and behavior, and are necessary for several aspects of sperm storage. For example, Acps cause a series of conformational changes in the mated female's reproductive tract that occur during and immediately after mating. These conformational changes have been hypothesized to aid both in the movement of sperm within the female and in the subsequent storage of those sperm. We used RNAi to systematically knock down several Acps involved in sperm storage to determine whether they played a role in the mating-induced uterine conformational changes. Mates of males lacking the glycoprotein Acp36DE, which is needed for the accumulation of sperm in the storage organs, fail to complete the full sequence of the conformational changes. Our results show that uterine conformational changes are important for proper accumulation of sperm in storage and identify a seminal protein that mediates these changes. Four Acps included in this study, previously shown to affect sperm release from storage (CG9997, CG1656, CG1652, and CG17575), are not necessary for uterine conformational changes to occur. Rather, consistent with their role in later steps of sperm storage, we show here that their presence can affect the outcome of sperm competition situations.reproduction ͉ seminal protein ͉ sperm competition ͉ sperm storage

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“…5A,AЈ), an enhancer-trap that reports Vesicular glutamate transporter (VGlut) gene expression (Mahr and Aberle, 2006). This was intriguing in light of reports of glutamatergic motoneuron innervation of the oviduct; type I-like neuromuscular junctions are present on the oviduct, which contracts vigorously in response to exogenous glutamate (Middleton et al, 2006;Rodríguez-Valentín et al, 2006;Kapelnikov et al, 2008).…”

Section: Research Article Female Bias Of Ilp7 Neuronsmentioning

confidence: 99%

“…After eggs exit the ovary, they are propelled through the oviduct by somatic-like muscles that ring the oviduct (Hudson et al, 2008). Peristaltic contraction/relaxation activity of these muscles is directed by unidentified excitatory glutamatergic motoneurons and inhibitory octopaminergic neurons (Middleton et al, 2006;Rodríguez-Valentín et al, 2006;Kapelnikov et al, 2008). Insulinlike peptide 7 (Ilp7)-expressing neurons are also reported to innervate the oviduct, and their electrical silencing blocks egg laying (Yang et al, 2008); yet, as Ilp7 mutants have no egg-laying phenotype (Grönke et al, 2010), the function of these neurons is uncertain.…”

Section: Introductionmentioning

confidence: 99%

Female-biased dimorphism underlies a female-specific role for post-embryonic Ilp7 neurons inDrosophilafertility

2013

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SUMMARYIn Drosophila melanogaster, much of our understanding of sexually dimorphic neuronal development and function comes from the study of male behavior, leaving female behavior less well understood. Here, we identify a post-embryonic population of Insulin-like peptide 7 (Ilp7)-expressing neurons in the posterior ventral nerve cord that innervate the reproductive tracts and exhibit a female bias in their function. They form two distinct dorsal and ventral subsets in females, but only a single dorsal subset in males, signifying a rare example of a female-specific neuronal subset. Female post-embryonic Ilp7 neurons are glutamatergic motoneurons innervating the oviduct and are required for female fertility. In males, they are serotonergic/glutamatergic neuromodulatory neurons innervating the seminal vesicle but are not required for male fertility. In both sexes, these neurons express the sex-differentially spliced fruitless-P1 transcript but not doublesex. The male fruitless-P1 isoform (fruM) was necessary and sufficient for serotonin expression in the shared dorsal Ilp7 subset, but although it was necessary for eliminating female-specific Ilp7 neurons in males, it was not sufficient for their elimination in females. By contrast, sex-specific RNA-splicing by female-specific transformer is necessary for female-type Ilp7 neurons in females and is sufficient for their induction in males. Thus, the emergence of female-biased post-embryonic Ilp7 neurons is mediated in a subset-specific manner by a tra-and fru-dependent mechanism in the shared dorsal subset, and a tra-dependent, fru-independent mechanism in the female-specific subset. These studies provide an important counterpoint to studies of the development and function of male-biased neuronal dimorphism in Drosophila.

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Tissue remodeling: a mating-induced differentiation program for the Drosophilaoviduct

Cited by 47 publications

References 57 publications

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae

Acp36DE is required for uterine conformational changes in mated Drosophila females

Female-biased dimorphism underlies a female-specific role for post-embryonic Ilp7 neurons inDrosophilafertility

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