Tissue Response in the Femur of Rabbits after Implantation of a New Calcium Titanium Phosphate Composition

Groß, Ulrich; Müller-Mai, C.; Voigt, Ch.; Mesgarian, M.; Berger, Georg; Ploska, Ute

doi:10.4028/www.scientific.net/kem.192-195.383

Cited by 4 publications

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“…The reduction of the solubility by the introduction of titanium results on a more stable material surface and local pH that contribute for a better cell behavior. In addition, two of the four phases present in the MT13B glass ceramics, namely, calcium pyrophosphate (β‐Ca 2 P 2 O 7 ) and calcium titanium phosphate [CaTi 4 (PO 4 ) 6 ] are reported to be biocompatible and bioactive 15, 30, 31…”

Section: Discussionmentioning

confidence: 99%

In vitro studies of calcium phosphate glass ceramics with different solubility with the use of human bone marrow cells

Dias

Lopes

Cabral

et al. 2005

J Biomedical Materials Res

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Two glass ceramics in the CaO--P2O5--MgO system with the incorporation of K2O or TiO2 oxides were prepared with the goal of using them as potential bone graft substitutes. The incorporation of TiO2 and K2O led to the preparation of specific crystalline phases in the structure of the glass ceramics, which show different degrees of biodegradation. In fact, the 45CaO--45P2O5--5MgO--5K2O has been previously demonstrated to be much more soluble in aqueous solutions than the 45CaO--37P2O5--5MgO--13TiO2 glass ceramic. The in vitro biological activity of the two calcium phosphate glass ceramics was studied with the use of human bone marrow osteoblast cell cultures maintained for 28 days, and seeded materials were assessed for cell proliferation and function. The Ti-containing glass ceramic showed a stable surface throughout the culture time, on macroscopic and SEM observation. Osteoblast cells proliferated gradually, especially during the third week, with a high alkaline phosphatase activity and formation of a mineralized matrix. On SEM observation, attached cells appeared with a spread-polygonal morphology typical of the osteoblast cells, with extensive cell-to-cell contact. Cell behavior on the seeded material was similar to that found on cultures performed on tissue-culture-grade polystyrene; except for the presence of lower cell numbers during the first 2 weeks. By contrast, the K-containing glass ceramic showed a highly instable surface with dissolution/precipitation processes occurring throughout the culture time. Few cells adhered to the material surface, and subsequent proliferation was also hindered, especially from the first week onwards. Cell numbers were significantly lower than those observed in the Ti-containing glass ceramic during most of the incubation time. Results suggest that the different in vitro biological behavior of these two glass ceramics is mainly due to the significant differences in the surface degradation rate, which is directly correlated to the chemical composition of the mother glass.

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Section: Discussionmentioning

confidence: 99%

In vitro studies of calcium phosphate glass ceramics with different solubility with the use of human bone marrow cells

Dias

Lopes

Cabral

et al. 2005

J Biomedical Materials Res

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“…The possibility of exploiting the capacity of both components to act as enzyme delivery systems was investigated, since both may immobilise proteins [23][24][25][26][27]. For this reason, the enzyme was incorporated into the ceramic-alginate matrix before gel formation in two different ways, as described before.…”

Section: Discussionmentioning

confidence: 99%

“…Its properties, namely the capacity of ion exchange and chemical adsorption [22], and the ability to act as an immobilisation matrix for several enzymes [23], suggest that it can successfully be used in the biomedical field. Furthermore, recent in vivo studies showed direct bone contact of implanted cylinders containing calcium titanium phosphate as the main phase [24]. HAp, which has long been recognised for its bioactivity and osteoconductive properties and has been extensively tested as matrix in drug delivery applications [25,26], was also used in the present investigation.…”

Section: Introductionmentioning

confidence: 99%

Calcium phosphate-alginate microspheres as enzyme delivery matrices

2004

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The present study concerns the preparation and initial characterisation of nov el calcium titanium phosphate-alginate (CTPalginate) and hydroxyapatite-alginate (HAp-alginate) microspheres, which are intended to be used as enzyme delivery matrices and bone regeneration templates. Microspheres were prepared using different concentrations of polymer solution (1% and 3% w/v) and different ceramic-to-polymer solution ratios (0.1, 0.2 and 0.4 w/w). Ceramic powders were characterised using X-ray diffraction, laser granulometry, Brunauer, Emmel and Teller (BET) method for the determination of surface area, zeta potential and Fourier transform infrared spectroscopy (FT-IR). Alginate was characterised using high performance size exclusion chromatography. The methodology followed in this investigation enabled the preparation of homogeneous microspheres with a uniform size. Studies on the immobilisation and release of the therapeutic enzyme glucocerebrosidase, employed in the treatment of Gaucher disease, were also performed. The enzyme was incorporated into the ceramic-alginate matrix before gel formation in two different ways: preadsorbed onto the ceramic particles or dispersed in the polymeric matrix. The two strategies resulted in distinct release profiles. Slow release was obtained after adsorption of the enzyme to the ceramic powders, prior to preparation of the microspheres. An initial fast release was achiev ed when the enzyme and the ceramic particles were dispersed in the alginate solution before producing the microspheres. The latter profile is very similar to that of alginate microspheres. The different patterns of enzyme release increase the range of possible applications of the system inv estigated in this work.

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“…Previously published [15,17,18] X-ray diffraction results showed that MT13B and MK5B glass ceramics were composed of several crystalline phases as shown in Table 1. Some of these phases, -Ca 2 P 2 O 7 and CaTi 4 (PO 4 ) 6 have already been reported to behave well in vivo, showing absence of signs of inflammation and supporting resorption and bone growth [19,20].…”

Section: Resultsmentioning

confidence: 94%

“…Some of these phases, -Ca 2 P 2 O 7 and CaTi 4 (PO 4 ) 6 have already been reported to behave well in vivo, showing absence of signs of inflammation and supporting resorption and bone growth [19,20].…”

Section: Resultsmentioning

confidence: 99%

In vivo Performance of Biodegradable Calcium Phosphate Glass Ceramics using the Rabbit Model: Histological and SEM Observation

et al. 2006

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Two MK5 (45CaO-45P 2 O 5 -5MgO-5K 2 O, in mol%) and MT13 (45CaO-37P 2 O 5 -5MgO-13TiO 2 , in mol%) glasses are prepared in the meta-and *Author to whom correspondence should be addressed. E-mail: jdsantos@fe.up.pt pyrophosphate regions and crystallized to obtain MK5B and MT13B, respectively. MK5B was obtained by controlled crystallization, and MT13B by powder sintering. As a result of these heat treatment processes, the crystalline phases precipitated in the glassy matrix are KCa(PO 3 ) 3 , -Ca(PO 3 ) 2 , -Ca 2 P 2 O 7 and Ca 4 P 6 O 19 phases for MK5B and CaTi 4 (PO 4 ) 6 , TiP 2 O 7 , -and -Ca 2 P 2 O 7 phases for MT13B. To assess the in vivo biological behavior of these glass ceramics, a mixed granulometry in the range 250-355 mm and 355-425 mm with a ratio of 1/1 was implanted for 2, 4, and 12 weeks in the tibiae of Japanese white rabbits. The results showed that the in vivo behavior was strongly affected by their solubility. All implanted materials, MK5B and MT13B, and -tricalcium phosphate (-TCP) as control material, showed signs of degradation in vivo. However, the levels of degradation were quite different throughout the implantation periods. The highest degradation was observed for MK5B glass ceramic and the lowest for MT13B with -TCP in-between. All implanted materials allow for new bone formation in the bone defect area. At the longest implantation period (12 weeks), the MT13B and -TCP materials were almost completely surrounded by new bone tissue, whereas MK5B showed some unfilled spaces. This behavior is discussed in terms of the high degradation observed in previous studies. JOURNAL OF BIOMATERIALS APPLICATIONS

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Tissue Response in the Femur of Rabbits after Implantation of a New Calcium Titanium Phosphate Composition

Cited by 4 publications

References 0 publications

In vitro studies of calcium phosphate glass ceramics with different solubility with the use of human bone marrow cells

In vitro studies of calcium phosphate glass ceramics with different solubility with the use of human bone marrow cells

Calcium phosphate-alginate microspheres as enzyme delivery matrices

In vivo Performance of Biodegradable Calcium Phosphate Glass Ceramics using the Rabbit Model: Histological and SEM Observation

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