Tissue-specific DNA cleavages in the globin chromatin domain introduced by DNAase I

Stalder, Jürg; Larsen, A. S.; Engel, James Douglas; Dolan, M. Eileen; Groudine, Mark; Weintraub, Harold

doi:10.1016/0092-8674(80)90631-5

Cited by 485 publications

(233 citation statements)

References 15 publications

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“…Restriction endonuclease digestion, blot hybridization, nick translation of probe fragments, and nuclear runoff transcription were all carried out as described previously (5,19,34). The restriction mapping of the HPFH breakpoint region and the molecular cloning of the probes used in these experiments have been described previously (19,23,26,31,37).…”

mentioning

confidence: 99%

Translocation of an erythroid-specific hypersensitive site in deletion-type hereditary persistence of fetal hemoglobin.

Elder¹,

Forrester²,

Thompson³

et al. 1990

Mol. Cell. Biol.

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Hereditary persistence of fetal hemoglobin (HPFH) can involve large deletions which eliminate the 3' end of the ,8-like globin gene cluster and more than 70 kilobases (kb) of flanking DNA. Blot hybridization revealed a DNase I-hypersensitive site extending from 1.1 to 1.4 kb downstream of the HPFH-1 3' deletion endpoint. The site was found in normal fetal and adult nucleated erythroid cells and in two erythroleukemia cell lines but not in nonerythroid cells and tissues. Simian virus 40 core enhancer-like sequences were found nonrandomly distributed within the boundaries of the site, which is contained in a fragment of known enhancer activity (E. A. Feingold and B. G. Forget, Blood, in press). A second hypersensitive site was found 0.5 kb upstream of the HPFH-1 3' deletion endpoint but was not erythroid specific. A third site, most prominent in fetal liver-derived erythroid cells, was found 1 kb upstream of the HPFH-2 deletion endpoint. As predicted by the locations of the deletion endpoints, the first two sites were translocated to within 12 kb of the A_Y gene in erythroid colonies derived from an HPFH-2 heterozygote and in hybrid mouse-human erythroid cells carrying the HPFH-2 deletion chromosome. Further analysis of this region showed that it was DNase I sensitive in erythroid and myeloid cells, indicating that it resides in an open chromatin domain. These observations suggest that alterations of chromatin structure flanking the fetal globin genes may contribute to abnormal gene regulation in deletion-type HPFH.

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mentioning

confidence: 99%

Translocation of an erythroid-specific hypersensitive site in deletion-type hereditary persistence of fetal hemoglobin.

Elder¹,

Forrester²,

Thompson³

et al. 1990

Mol. Cell. Biol.

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“…To initiate the production of monoclonal antibodies directed specifically against chicken red blood cell nuclear proteins, we immunized mice with urea-soluble material (method A) from 14-day-old embryonic chicken erythrocyte nuclei. Most ofthe cells in this population contain adult hemoglobin and are mature erythrocytes derived from the definitive erythrocyte lineage (19). The urea-soluble material from such nuclear preparations contained a large spectrum of nonhistone components ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Tissue-specific and species-specific monoclonal antibodies to avian red cell nuclear proteins.

Kane¹,

Cheng²,

Burch³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

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“…Recently the DNase I sensitivity of specific genes in the chromatin has been investigated using a more refined technique in which the digestion of specific restriction fragments of defined genes was measured following DNase I treatment of chromatin (3,4). Qualitative evaluation of such experiments supported the general hypothesis that an active gene and its flanking sequences are more rapidly digested by DNase I than an inactive gene (5,6,7,8) and furthermore demonstrated the existence of hypersensitive DNase I sites in the chromatin of some of the genes analyzed (3, ©) IRL Prm Umited, 1 Falconberg Court, London W1V 5FG, U.K.…”

Section: Introductionmentioning

confidence: 89%

“…In these experiments the DNase I sensitivity has been investigated by measuring the residual globin sequences still hybridizing with globin cDNA after extensive DNase I digestion. Applying the same approach which we have used, recent experiments made on chicken chromatin have demonstrated that defined globin restriction fragments were preferentially digested by DNase I in nuclei from erythrocytes compared to non-erythropoietic tissues (5,6,7). We have also analyzed the DNase I sensitivity of an albumin gene as this gene is expressed in hepatocytes but not in erythrocytes and found that the albumin gene is more sensitive to DNase I in chromatin of the tissue in which it is expressed.…”

mentioning

confidence: 99%

Quantitation of DNase I sensitivity in Xenopus chromatin containing active and inactive globin, albumin and vitellogenin genes

Felber¹,

Gerber-Huber²,

Meier

et al. 1981

Nucl Acids Res

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The disappearance of defined restriction fragments of the a1-globin, an albumin and the Al vitellogenin gene was quantitated after DNase I digestion and expressed by a sensitivity factor defined by a mathematical model. Analysis of naked DNA showed that the gene fragments have similar but not identical sensitivity factors. DNase I digestion of chromatin revealed for the same gene fragments sensitivity factors differing over a much wider range. This is correlated to the activity of the genes analyzed: the a1-globin gene fragment is more sensitive to DNase I in chromatin of erythrocytes compared to hepatocytes whereas the albuimin gene fragment is more sensitive to DNase I in chromatin of hepatocytes. The Al vitellogenin gene has the same DNase I sensitivity in both cell types. Comparing the DNase I sensitivity of the three genes in their inactive state we suggest that different chromatin conformations may exist for inactive genes.

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Tissue-specific DNA cleavages in the globin chromatin domain introduced by DNAase I

Cited by 485 publications

References 15 publications

Translocation of an erythroid-specific hypersensitive site in deletion-type hereditary persistence of fetal hemoglobin.

Translocation of an erythroid-specific hypersensitive site in deletion-type hereditary persistence of fetal hemoglobin.

Tissue-specific and species-specific monoclonal antibodies to avian red cell nuclear proteins.

Quantitation of DNase I sensitivity in Xenopus chromatin containing active and inactive globin, albumin and vitellogenin genes

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