Tissue-specific enhancement of xenobiotic detoxification enzymes in mice by dietary rosemary extract

Singletary, Keith W.; Rokusek, Joan T.

doi:10.1007/bf02436042

Cited by 39 publications

(22 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is generally assumed that these antioxidant compounds may scavenge free radicals and regulate the activity and/or expression of certain enzymatic systems implicated in relevant physiological processes like the metabolism of xenobiotics in the liver (Singletary and Rokusek, 1997;Subbaramaiah et al, 2002). Synergistic interactions amongst the antioxidants present in the HE of R. officinalis leaves might reduce serum ALT, ALP, and AST levels.…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective effect of Rosmarinus officinalis and rosmarinic acid on acetaminophen-induced liver damage

Lucarini

Bernardes

Tozatti

et al. 2014

Emir. J. Food Agric

View full text Add to dashboard Cite

The hydroalcoholic extract of the leaves of Rosmarinus officinalis (HE), a species of medicinal value, and its constituent rosmarinic acid (RA) were evaluated for hepatoprotective in the acetaminophen-induced liver damage model. Groups of Wistar albino rats (n=6) were pre-administered with HE (100, 250 and 500 mg/kg, p.o.) and RA (10, 25 and 50 mg/kg, p.o.) prior to a single dose of acetaminophen (APAP, 600 mg/kg body weight; p.o). The hepatoprotective activity of HE extract was observed through histopathological analysis and reduction of the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). These observations were comparable to the normal group prior to APAP administration. Group that was treated with APAP alone exhibited high levels of transaminases and ALP. The obtained results from the present study suggested that HE may prevent APAP-induced hepatic injuries. RA did not displayed significant hepatoprotective activity against APAP-induced liver damage.

show abstract

Section: Discussionmentioning

confidence: 99%

Hepatoprotective effect of Rosmarinus officinalis and rosmarinic acid on acetaminophen-induced liver damage

Lucarini

Bernardes

Tozatti

et al. 2014

Emir. J. Food Agric

View full text Add to dashboard Cite

show abstract

“…Earlier studies reported that rosemary extracts inhibited 7,12-dimethyl-benz[a]anthracene- (43). Some of the protective effects of rosemary extracts were attributed to enhancement of xenobiotic detoxification (44).…”

Section: Discussionmentioning

confidence: 99%

Rosmarinic Acid Antagonizes Activator Protein-1–Dependent Activation of Cyclooxygenase-2 Expression in Human Cancer and Nonmalignant Cell Lines

Scheckel

Degner

Romagnolo

2008

The Journal of Nutrition

121

View full text Add to dashboard Cite

One mechanism through which bioactive food components may exert anticancer effects is by reducing the expression of the proinflammatory gene cyclooxygenase-2 (COX-2), which has been regarded as a risk factor in tumor development.Rosmarinic acid (RA) is a phenolic derivative of caffeic acid present in rosemary (Rosmarinus officinalis). Previous research documented that RA may exert antiinflammatory effects. However, the mechanisms of action of RA on COX-2 expression have not been investigated. Here, we report that in colon cancer HT-29 cells, RA (5, 10, and 20 mmol/L) reduced the 12-Otetradecanoylphorbol-13-acetate (TPA)-induced COX-2 promoter activity (P , 0.05) and protein levels (P , 0.05). In addition, the cotreatment with RA reduced (5 mmol/L, P , 0.05; 10 and 20 mmol/L, P , 0.01) TPA-induced transcription from a control activator protein-1 (AP-1) promoter-luciferase construct and repressed binding of the AP-1 factors c-Jun (10 mmol/L; P , 0.01) and c-Fos (10 mmol/L; P , 0.05) to COX-2 promoter oligonucleotides harboring a cAMP-response element (CRE). The anti-AP1 effects of RA were also examined in a nonmalignant breast epithelial cell line (MCF10A) in which RA antagonized the stimulatory effects of TPA on COX-2 protein expression (5 mmol/L, P , 0.05; 10 and 20 mmol/L, P , 0.01), the recruitment of c-Jun and c-Fos (10 mmol/L; P , 0.01) to the COX-2/CRE oligonucleotides, and activation of the extracellular signal-regulated protein kinase-1/2 (ERK1/2) (10 mmol/L; P , 0.01), a member of the mitogen-activated protein kinase pathway. Additionally, RA antagonized ERK1/2 activation in colon HT-29 and breast MCF-7 cancer cells (10 mmol/L; P , 0.01). Thus, we propose that RA may be an effective preventative agent against COX-2 activation by AP-1-inducing agents in both cancer and nonmalignant mammary epithelial cells.

show abstract

“…Polyphenols are one such agent which can modulate cell growth and differentiation and interfere with cancer development and progression [6]. Several studies indicate that extracts of the plant rosemary (Rosemarinus officinalis), which is rich in polyphenols, inhibit both tumour initiation and progression in mice and rat models due to its inherent high anti-oxidant activity [7,8]. Carnosic acid (CA), the major polyphenolic diterpene of rosemary, has been shown to possess anti-inflammatory as well as antiproliferative activity in HL-60 and U937 myeloblastic cells [9,10].…”

Section: Introductionmentioning

confidence: 99%

Carnosic acid modulates Akt/IKK/NF-κB signaling by PP2A and induces intrinsic and extrinsic pathway mediated apoptosis in human prostate carcinoma PC-3 cells

et al. 2012

View full text Add to dashboard Cite

This study investigates the efficacy of carnosic acid (CA), a polyphenolic diterpene, isolated from the plant rosemary (Rosemarinus officinalis), on androgen-independent human prostate cancer PC-3 cells. CA induced anti-proliferative effects in PC-3 cells in a concentration- and time-dependent manner, which was due to apoptotic induction as evident from flow-cytometry, DNA laddering and TUNEL assay. Apoptosis was associated with the activation of caspase-8, -9, -3 and -7, increase in Bax:Bcl-2 ratio, release of cytochrome-c and decrease in expression of inhibitor of apoptosis (IAP) family of proteins. Apoptosis was attenuated upon pretreatment with specific inhibitors of caspase-8 (Z-IETD-fmk) and caspase-9 (Z-LEHD-fmk) suggesting the involvement of both intrinsic and extrinsic apoptotic cascades. Further, apoptosis resulted from the inhibition of IKK/NF-κB pathway as evident from decreased DNA binding activity, nuclear translocation of p50 and p65 and IκBα phosphorylation. The down-regulation of IKK/NF-κB was associated with inhibition of Akt phosphorylation and its kinase activity with a concomitant increase in the serine/threonine protein phosphatase 2A (PP2A) activity. Pharmacologic inhibition of PP2A by okadaic acid and calyculin A, significantly reversed CA-mediated apoptotic events in PC-3 cells indicating that CA induced apoptosis by activation of PP2A through modulation of Akt/IKK/NF-κB pathway. In addition, CA induced apoptosis in another androgen refractory prostate cancer DU145 cells via intrinsic pathway as evidenced from the activation of caspase 3, cleavage of PARP, increase in Bax:Bcl-2 ratio and cytochrome-c release. Carnosic acid, therefore, may have the potential for use in the prevention and/or treatment of prostate cancer.

show abstract

Tissue-specific enhancement of xenobiotic detoxification enzymes in mice by dietary rosemary extract

Cited by 39 publications

References 8 publications

Hepatoprotective effect of Rosmarinus officinalis and rosmarinic acid on acetaminophen-induced liver damage

Hepatoprotective effect of Rosmarinus officinalis and rosmarinic acid on acetaminophen-induced liver damage

Rosmarinic Acid Antagonizes Activator Protein-1–Dependent Activation of Cyclooxygenase-2 Expression in Human Cancer and Nonmalignant Cell Lines

Carnosic acid modulates Akt/IKK/NF-κB signaling by PP2A and induces intrinsic and extrinsic pathway mediated apoptosis in human prostate carcinoma PC-3 cells

Contact Info

Product

Resources

About