Tissue-Specific Expression in Transgenic Mice of a Fused Gene Containing RSV Terminal Sequences

Overbeek, Paul A.; Lai, Sing-Ping; Quill, K.R. Van; Westphal, Heiner

doi:10.1126/science.3006249

Cited by 149 publications

(65 citation statements)

References 28 publications

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“…The RSV promoter led to a preferential expression in epidermal derivatives and epithelial tissues derived from tbe mesoderm. The latter findings is in agreement with results in transgenic mice (Overbeek et al 1986) while epidermal expression directed by the RSV-LTR has not been reported so far in in vivo gene transfer experiments.…”

Section: Discussionsupporting

confidence: 82%

Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes)

Winkler

Vielkind²,

Schartl

1991

Mol Gen Genet

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Summary. Species of small fish are becoming useful tools for studies on vertebrate development. Wehave investigated the developing embryo of the Japanese medaka for its application as a transient expression system for the in vivo analysis of gene regulation and function. The temporaland spatial expression patterns ofbacterial chloramphenicol acetyltransferase and galactosidase reporter genes injected in supercoiled plasmid form into the cytoplasm of one cell of the two-cell stage embryo was promoter-specific. The transient expression was found to be mosaic within the tissue and organs reflecting the unequal distribution of extrachromosomal foreign DNA and the intensive cell mixing movements that occur in fish embryogenesis. The expression data are consistent with data on DNA fate. Foreign DNA persisted during embryogenesis and was still detectable in some 3-and 9-month-old adult fish; it was found in high molecular weight form as weil as in circular plasmid conformations. The DNA was replicated during early and late embryogenesis. Our data indicate that the developing medaka embryo is a powerful in vivo assay system for studies of gene regulation and function.

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Section: Discussionsupporting

confidence: 82%

Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes)

Winkler

Vielkind²,

Schartl

1991

Mol Gen Genet

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“…Moreover, this system permits the delivery of functional genes from the circulation. This ability of the anti-SC Fab-based carrier to transfect only epithelial and submucosal gland cells in the airways may be an important advantage, since the approach from the blood may permit more uniform distribu- (37,38).…”

Section: Resultsmentioning

confidence: 99%

Gene transfer into the airway epithelium of animals by targeting the polymeric immunoglobulin receptor.

et al. 1995

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(1,5). However, when the same conjugates were used to introduce exogenous genes into cotton rats via the intratracheal route, the transgene was transiently expressed at low levels ( 12).We have demonstrated that in primary cultures of human tracheal epithelial cells, targeting the polymeric immunoglobulin receptor (pIgR)' can be used for the specific delivery of reporter genes to cells that bear the receptor (2). This receptor undergoes efficient internalization and is specifically adapted for the nondegradative transfer of large molecules. In addition, the cellular distribution of pIgR expression in the surface epithelium and serous cells of the submucosal glands conforms to that of the cystic fibrosis transmembrane conductance regulator in human airways (13,14). In this report, we show that tar-1. Abbreviations used in this paper: IL2r, human interleukin 2 receptor; pIgR, polymeric immunoglobulin receptor; SC, secretory component; SPDP, N-Succinimidyl 3-(2-pyridyldithio) proprionate; X-gal, 5-Bromo4-chloro-3-indolyl-,8-galactopyranoside.Gene Transfer into Respiratory Epithelial Cells In Vivo 493 J. Clin. Invest.

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“…Residual CAT activity was measured in homogenates of pancreas from hyperglycemic diabetic animals with blood glucose levels >400 mg/dl. To control for effects of the streptozotocin treatment on the CAT reporter, we tested mice carrying a Rous sarcoma virus-CAT [RSV/CAT)construct [Overbeek et al 1986). To control for the response of the elastase promoter alone, we examined the effect of streptozotocin on transgenic mice with the elastase-CAT gene {con-struct 21.…”

Section: Reduced Expression In Diabetic Micementioning

confidence: 99%

Regulation of amylase gene expression in diabetic mice is mediated by a cis-acting upstream element close to the pancreas-specific enhancer.

Keller¹,

Rosenberg²,

Johnson³

et al. 1990

Genes Dev.

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We localized the c/s-acting sequences that mediate the regulation of a pancreatic amylase gene, Amy-2.2, in diabetic mice. We constructed three hybrid genes containing sequences from the 5'-flanking region of the amylase gene upstream of the heterologous elastase promoter linked to the CAT structural gene. These constructs were transferred to the germ line of transgenic mice by microinjection of fertilized eggs. The amylase sequences had two effects on expression of the elastase promoter: Basal expression was increased, and expression in diabetic animals was reduced to -~2% of basal levels. A 30-bp amylase fragment was sufficient to transfer both of these regulatory functions to the elastase promoter. Sequences within this 30-bp fragment are included in the binding site for the pancreatic nuclear protein PTFI. The close association of the PTFl-binding site and the regulatory functions is consistent with a mechanism based on interference with activation by PTFI in diabetic pancreas. PTFl-binding activity is not reduced in diabetic pancreas. The data presented here demonstrate that the 5'-flanking region of the pancreatic amylase gene contains a novel insulin-dependent element (IDE) that mediates the loss of expression in diabetic animals.

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Tissue-Specific Expression in Transgenic Mice of a Fused Gene Containing RSV Terminal Sequences

Cited by 149 publications

References 28 publications

Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes)

Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes)

Gene transfer into the airway epithelium of animals by targeting the polymeric immunoglobulin receptor.

Regulation of amylase gene expression in diabetic mice is mediated by a cis-acting upstream element close to the pancreas-specific enhancer.

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