Tissue Specific Expression of Glutathione S-transferases, Glutathione Content and Lipid Peroxidation in Camel Tissues

Raza, Haider; Lakhani, M.S.; Ahmed, Ijaz; John, Annie; Morgenstern, Ralf; Montague, W.

doi:10.1016/s0305-0491(97)00234-4

Cited by 14 publications

(8 citation statements)

References 23 publications

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“…This enzyme catalyzes the conjugation of GSH with a large variety of toxic and carcinogenic compounds [38]. GSH and its regenerating enzyme GR, were suppressed in Pb-intoxicated rats (Tables 2 and 3).…”

Section: Effect Of Leadmentioning

confidence: 97%

Prophylactic effect of melatonin on lead-induced inhibition of heme biosynthesis and deterioration of antioxidant systems in male rats

El-Missiry

2000

J. Biochem. Toxicol.

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We studied the protective role of the pineal hormone melatonin on lead‐induced suppression of the heme synthesis pathway as a consequence of reduced antioxidant systems in rat. We injected rats intramuscularly with lead acetate (10 mg/kg body weight) daily for 7 days, which significantly abolished heme synthesis as evidenced by decreased blood hemoglobin, liver δ‐aminolevulinic acid synthetase, erythrocytic δ‐aminolevulinic acid dehydratase, and hepatic iron content. These effects were accompanied with marked elevation of hepatic lipid peroxidation and decreased enzymatic antioxidants such as glutathione reductase, glutathione‐S‐transferase, superoxide dismutase, and catalase, as well as nonenzymatic antioxidants such as total sulfhydryl groups and glutathione. Furthermore, lead treatment caused hepatic deficiency in copper and zinc accompanied by a significant elevation of lead concentration in both plasma and liver. Daily pretreatment with melatonin (30 mg/kg body weight) intragastrically prevented the suppressive effects of lead on heme‐synthesizing enzymes and iron deficiency. In addition, preadministration of melatonin reduced the inhibitory effect of lead on both enzymatic and nonenzymatic antioxidants. This was accompanied by marked normalization of lipid peroxidation and modulation of copper and zinc levels in liver. The action of melatonin on lead‐induced changes was attributed to protection of the antioxidant capacity in cells in addition to the ability of melatonin to scavenge free radicals. © 1999 John Wiley & Sons, Inc. J Biochem Toxicol 14: 57–62, 2000

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Section: Effect Of Leadmentioning

confidence: 97%

Prophylactic effect of melatonin on lead-induced inhibition of heme biosynthesis and deterioration of antioxidant systems in male rats

El-Missiry

2000

J. Biochem. Toxicol.

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“…The sections were then incubated for 30 min in a solution of hydrogen peroxide (0.3% v/v in methanol) to quench endogenous peroxidase activity. Treatment with VEGF, Cyclin D1 primary antibodies, HRP coupled secondary antibody and immunohistochemical visualization of stained region was performed as described previously [24].…”

Section: Isolation Of Tissues and Immunohistochemical Localization Ofmentioning

confidence: 99%

Antitumour activity of Lycium chinensis polysaccharides in liver cancer rats

Cui

Chen

Liu

et al. 2012

International Journal of Biological Macromolecules

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“…At the end of 21 weeks of diabetes, the animals were sacrificed and small portions of the liver, kidney and testis were immediately removed and fixed overnight in freshly prepared Zamboni's fixative as described earlier (Raza et al, 1997). Representative fragments from control and diabetic rats were taken from similar areas of the organs.…”

Section: Isolation Of Tissues and Immunohistochemical Localization Ofmentioning

confidence: 99%

“…The sections were then incubated for 30 min in a solution of hydrogen peroxide (0.3% v/v in methanol) to quench endogenous peroxidase activity. Treatment with GST primary antibodies, HRP coupled secondary antibody and immunohistochemical visualization of stained region was performed as described previously (Raza et al, 1997). Table 1 shows the level of hyperglycemia and oxidative stress in diabetic rats one week after the STZ treatment.…”

Section: Isolation Of Tissues and Immunohistochemical Localization Ofmentioning

confidence: 99%

Tissue specific expression and immunohistochemical localization of glutathione S-transferase in streptozotocin induced diabetic rats: Modulation by Momordica charantia (karela) extract

Raza¹,

Ahmed²,

John³

2004

Life Sciences

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Tissue Specific Expression of Glutathione S-transferases, Glutathione Content and Lipid Peroxidation in Camel Tissues

Cited by 14 publications

References 23 publications

Prophylactic effect of melatonin on lead-induced inhibition of heme biosynthesis and deterioration of antioxidant systems in male rats

Prophylactic effect of melatonin on lead-induced inhibition of heme biosynthesis and deterioration of antioxidant systems in male rats

Antitumour activity of Lycium chinensis polysaccharides in liver cancer rats

Tissue specific expression and immunohistochemical localization of glutathione S-transferase in streptozotocin induced diabetic rats: Modulation by Momordica charantia (karela) extract

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