Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds

Pan, Zhangyuan; Di, Ran; Tang, Qing; Jin, Hai; Chu, Mingxing; Huang, Dali; He, Jianning; Liu, Q. Y.; Hu, Wenping; Wang, X. Y.; Yao, Yilong; Liu, L.; Song, Chunyan

doi:10.17221/8525-cjas

Cited by 19 publications

(21 citation statements)

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“…Sheep carrying the FecB mutation exhibit higher ovulation numbers [ 11 ], which may be the cause of the different litter sizes of sheep in the three FecB genotypes. Moreover, the expression differences in BMPR1B in non-ovarian tissues of the HPG axis of sheep of the three FecB genotypes were not consistent with the results from goat breeds [ 35 ]. We infer that the expression profile described above may have a species discrepancy or the different timings of the sampling may have influenced the results.…”

Section: Discussionmentioning

confidence: 82%

“…In addition, some researchers have found that BMPR1B has no tissue-specific expression in whole tissues, and it was also shown to have a high expression level in the ovaries of the Jining Grey goat and Liaoning Cashmere goat, implying that it not only exerts a great influence on the process of bone formation, but also has a potential role in ovarian development and even in the process of reproduction. However, no significant difference in expression was shown between these two goat breeds, which suggested that the BMPR1B may not contribute to the high fecundity of goats [ 35 ]. Similarly, it has also been speculated that differences in reproductive performance may be ascribed to the structural differences in BMPR1B between Lezhi black and Tibetan goats [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, BMP15 was shown to be highly expressed in the ovaries, with trace expression in five other tissues (spleen, lung, kidney, hypothalamus, and oviduct), which is consistent with the results in Hu sheep [ 46 ]. Previous research indicated that BMP15 can downregulate the follicle stimulating hormone (FSH) effect, promote cell proliferation, and ensure the growth and maturity of oocytes by inhibiting the expression of the FSH receptor [ 35 ]. Therefore, BMP15 , based on its high expression in the ovary, may be an important factor in maintaining numerous follicular developments.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it can be inferred that ewes with the FecB mutant have a high ovulation rate, which may be responsible for the low expression of BMP15 in the ovaries of Han BB sheep. However, some studies have also concluded that BMP15 is a key gene in the high fecundity of goats based on results where BMP15 expression in the hircine ovary was shown to be significantly higher in a polytocous breed than in a monotocous breed [ 35 , 47 ]. These studies suggest that BMP15 has a substantial regulatory effect on the ovulation rates of sheep and goat [ 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

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Expression Analysis of the Prolific Candidate Genes, BMPR1B, BMP15, and GDF9 in Small Tail Han Ewes with Three Fecundity (FecB Gene) Genotypes

Tang

et al. 2018

Animals

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Simple SummaryAs important prolific candidate genes, BMPR1B, BMP15, and GDF9 may affect the lambing performance of sheep. Therefore, regarding the three FecB genotypes of Small Tail Han (STH) sheep (FecB BB, FecB B+, and FecB ++), this study explored the gene expression characteristics of different tissues using reverse transcription PCR (RT-PCR) and real-time quantitative PCR (qPCR). The results showed that BMPR1B, BMP15, and GDF9 expression differed between the selected tissues, with all being highly expressed in the ovaries. Further analysis indicated that there was no significant difference in BMPR1B expression among the three FecB genotypes, but both GDF9 and BMP15 had the highest expression in FecB B+. As for other non-ovarian tissues, expression also varied. This study is relevant to understanding the high prolificacy of the STH breed.AbstractThe expression characteristics of the prolific candidate genes, BMPR1B, BMP15, and GDF9, in the major visceral organs and hypothalamic–pituitary–gonadal (HPG) axis tissues of three FecB genotypes (FecB BB, FecB B+, and FecB ++) were explored in STH ewes using RT-PCR and qPCR. The results were as follows, BMPR1B was expressed in all FecB BB genotype (Han BB) tissues, and GDF9 was expressed in all selected tissues, but BMP15 was specifically expressed in the ovaries. Further study of ovarian expression indicated that there was no difference in BMPR1B expression between genotypes, but the FecB B+ genotype (Han B+) had greater expression of GDF9 and BMP15 than Han BB and FecB ++ genotype (Han ++) (p < 0.05, p < 0.01). BMP15 expression was lower in the ovaries of Han BB than in Han ++ sheep, but the reverse was shown for GDF9. The gene expression in non-ovarian tissues was also different between genotypes. Therefore, we consider that the three genes have an important function in ovine follicular development and maturation. This is the first systematic analysis of the tissue expression pattern of BMPR1B, BMP15, and GDF9 genes in STH sheep of the three FecB genotypes. These results contribute to the understanding of the molecular regulatory mechanism for ovine reproduction.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Expression Analysis of the Prolific Candidate Genes, BMPR1B, BMP15, and GDF9 in Small Tail Han Ewes with Three Fecundity (FecB Gene) Genotypes

Tang

et al. 2018

Animals

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“…The level of GAPDH gene expression served as an internal control. The quantitative real-time PCR data were calculated by the 2 -∆∆Ct method (Pan et al 2015). Amplified products were separated by electrophoresis on a 2% agarose gel and detected under UV light.…”

Section: Doi: 1017221/42/2016-cjasmentioning

confidence: 99%

Retinoic acid attenuates oxidative injury in bovine mammary epithelial cells induced by hydrogen peroxide

Jin¹,

Yan²,

Shi³

et al. 2017

CZECH J ANIM SCI

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Jin L., Yan S., Shi B., Shi H., Guo X., Li J. (2017): Retinoic acid attenuates oxidative injury in bovine mammary epithelial cells induced by hydrogen peroxide. Czech J. Anim. Sci., 62, 539-548.The objective of this study was to explore how retinoic acid (RA) attenuates oxidative injury induced by hydrogen peroxide (H 2 O 2 ) in bovine mammary epithelial cells (BMEC). Subconfluence BMEC were randomly divided into four groups with six replicates: the control group (incubated in serum-free medium without RA or H 2 O 2 for 30 h), H 2 O 2 group (pre-incubated for 24 h without RA, then for another 6 h with 600 μM H 2 O 2 ), RA group (incubated with 1 mg/ml RA for 30 h without H 2 O 2 ), and RA + H 2 O 2 group (RA prevention group, pre-incubated with 1 mg/ml RA for 24 h and then for another 6 h with 600 mM H 2 O 2 ). The results showed that the H 2 O 2 treatment significantly decreased several measured traits, including the cell viability, glutathione peroxidase (GPX) and thioredoxin reductase (TRXR) activities, selenoprotein P (SELP) content, catalase and superoxide dismutase activities, total antioxidant capacity, and GPX1, TRXR1, and SELP gene expression, as well as GPX1 and TRXR1 protein expression. H 2 O 2 treatment also increased the malondialdehyde and reactive oxygen species contents and induced a marked increase of several measured traits, including the arachidonic acid (ARA) concentration, cytosolic phospholipase A2 and 5-lipoxygenase gene expression and activity, and 15-hydroxy twenty-four arachidonic acid and hydroxy peroxide tetracosenic arachidonic acid contents. RA pre-treatment prevented corresponding increases in parameters related to ARA metabolism and increased the activity of TRXR. Moreover, RA pre-treatment attenuated the phosphorylation levels of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase and effectively decreased the ARA content. These results suggest that RA protected BMEC from oxidative stress by elevating TRXR activity, which inhibited the MAPK signaling pathway and led to a decreased concentration of ARA.Keywords: vitamin A; thioredoxin reductase; oxidative injury; arachidonic acid; p38 mitogen-activated protein kinase List of abbreviations: RA = retinoic acid, ROS = reactive oxygen species, VA = vitamin A, BMEC = bovine mammary epithelial cells, GPX1 = glutathione peroxidase 1, TRXR1 = thioredoxin reductases 1, ARA = arachidonic acid, CPLA2 = cytosolic phospholipase A2, MAPK = mitogen-activated protein kinase, H 2 O 2 = hydrogen peroxide, DMSO = dimethyl sulfoxide, MTT = 5-diphenyltetrazolium bromide, DMEM/F12 = Dulbecco's Modified Eagle's Medium/F12, 5-LOX = 5-lipoxygenase, PGE2 = prostaglandin E2, COX-2 = cyclooxygenase-2, LTB4 = leukotriene B4, 12-or 15-HETE = 12-or 15-hydroxyeicosatetraenoic acid, 12-or 15-HPETE = 12-or 15-hydroperoxyeicosatetraenoic acid, ERK1/2 = extracellular signal-regulated kinase ½, JNK = c-Jun N-terminal kinase, PBS = phosphate buffered solution, RGR = relative growth rate, T-AOC = total antioxidant capacity, SOD = superoxide ...

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qPCR and HRM-based diagnosis of SNPs on growth differentiation factor 9 (GDF9), a gene associated with sheep (Ovis aries) prolificacy

et al. 2017

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Prolificacy is a desirable trait for genetic improvement of sheep flocks, since it holds the potential to improve productivity. Animals carrying single-nucleotide polymorphisms (SNPs) in genes associated with this trait can be identified and employed to increase prolificacy in flocks. In this study, we report a diagnostic method based on quantitative PCR and high-resolution melting curves to detect different SNPs in the prolificacy-associated gene growth differentiation factor 9 (GDF9). The diagnostic method was validated using artificial sequences representing known SNPs in GDF9, then applied to a real flock comprising four breeds and admixed animals (n = 306). Five different SNPs were identified in this flock, as was a low or null frequency of occurrence of SNPs positively associated with prolificacy. This indicates a need to implement a breeding strategy for recovering or reintroducing such SNPs. Our method provides a genotyping strategy for identifying individuals with SNPs of interest for prolificacy, which will help producers plan a breeding strategy for this trait. This method can be adapted and expanded for the diagnosis of other traits of interest.

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Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds

Cited by 19 publications

References 32 publications

Expression Analysis of the Prolific Candidate Genes, BMPR1B, BMP15, and GDF9 in Small Tail Han Ewes with Three Fecundity (FecB Gene) Genotypes

Expression Analysis of the Prolific Candidate Genes, BMPR1B, BMP15, and GDF9 in Small Tail Han Ewes with Three Fecundity (FecB Gene) Genotypes

Retinoic acid attenuates oxidative injury in bovine mammary epithelial cells induced by hydrogen peroxide

qPCR and HRM-based diagnosis of SNPs on growth differentiation factor 9 (GDF9), a gene associated with sheep (Ovis aries) prolificacy

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