Tissue-specific targeting of Hsp26 has no effect on heat resistance of neural function in larval Drosophila

Abstract

Hsp26 belongs to the small heat-shock protein family and is normally expressed in all cells during heat stress. We aimed to determine if overexpression of this protein protects behavior and neural function in Drosophila melanogaster during heat stress, as has previously been shown for Hsp70. We used the UAS-GAL4 expression system to drive expression of Hsp26 in the whole animal (ubiquitously), in the motoneurons, and in the muscles of wandering third-instar larvae. There were slight increases in time to crawling failure and normalized excitatory junction potential (EJP) area for some of the transgenic lines, but these were not consistent. In addition, Hsp26 had no effect on the temperature at failure of EJPs, normalized EJP peak amplitude, and normalized EJP half-width. Overexpression larvae had a similar number of motoneuronal boutons and length of nerve terminals as controls, indicating that the occasional protective effects on locomotion were not due to changes at the synapse. We conclude that overexpression had a small thermoprotective effect on locomotion and no effect on neural function. As it has been shown that Hsp26 requires action of other Hsps to reactivate the denatured proteins to which it binds, we propose that at least in larvae, the function of Hsp26 was masked in the relative absence of other Hsps.

Drosophila Small Heat Shock Proteins: An Update on Their Features and Functions

“…The matter of timing has also been reported in other studies. For example, the degree of the protective effect of Hsp26 over-expression is higher in adults than in larvae where it has only a small thermoprotective effect on larval locomotion and no effect on neural function (Liao et al 2008 ;Mileva-Seitz et al 2008 ;Wang et al 2004 ).…”