Titanium dioxide–mediated resistive nanobiosensor for E. coli O157:H7

Nadzirah, Sh.; Hashim, U.; Gopinath, Subash C.B.; Parmin, N. A.; Hamzah, Azrul Azlan; Yu, Hung Wei; Dee, Chang Fu

doi:10.1007/s00604-020-4214-y

Cited by 19 publications

(11 citation statements)

References 39 publications

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“…respectively. The experimentally established LOD for both targets was 10 -19 M which is significantly lower when compared to similar electrochemical sensors for the detection of nucleic acids developed recently [27,[31][32][33][34][35]. The reproducibility of the sensor was calculated for 100 aM (10 -16 M) and 10 fM (10 -14 M) concentrations using the stx1 probe and the relative standard deviation (RSD%) was 12% and 8%, respectively (n=6).…”

Section: Multiplex Sensor Developmentmentioning

confidence: 85%

“…However, the complex samples tested by Kashish et al [25] were with the use of PCR amplicons, which means an additional amplification step had to be introduced. In another study, STEC on contaminated chicken samples was directly detected using a sensor developed by Nadzirah et al [27] using interdigitated electrodes (IDEs); which have gained much attention recently [28,29]. Aluminium IDEs modified with TiO2 and APTES were used to immobilise probe DNA and the hybridisation event was detected by conductance.…”

Section: Introductionmentioning

confidence: 99%

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Amplification-free, highly sensitive electrochemical DNA-based sensor for simultaneous detection of stx1 and stx2 genes of Shiga toxin-producing E. coli (STEC)

O’Riordan

Wasiewska

Diaz

et al. 2022

Preprint

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Shiga toxin-producing E. coli (STEC) is a food-borne pathogen of significant public health concern, due to the severity of the illness it can cause and a low infection dose. The key targets in molecular-based assays to detect STEC are stx1 or stx2 genes, coding for the ability to produce Shiga T Toxin 1 and 2. The most commonly used molecular detection techniques, such as PCR and real-time PCR, are considerably time-consuming and there is an urgent need for a more rapid screening assay which could be used in agri-food settings. In this work, an amplification-free multiplex electrochemical sensor for the simultaneous detection of stx1 and stx2 genes was developed using a silicon-based chip comprising of six interdigitated gold microelectrodes(IDE) sensors. Two probes complementary to stx1 and stx2 genes were immobilised on a single chip allowing for multiplex detection. The selectivity of the multiplex sensor was confirmed using fluorescence and electrochemistry. The developed assay conditions allowed for an improved limit of detection by three orders of magnitude compared to the previous reports achieving a amplification-free limit of detection of 100 zM (10-19 M) for both, stx1 and stx2 genes. The developed sensor was validated using chromosomal DNA extracted from a bacterial culture containing no virulence genes, stx1 gene only, and both stx1 and stx2. Such multiplex sensor, if combined with on-chip DNA extraction, could revolutionise the point-of-use detection of STEC as well as other pathogens for instance on-farm or in the food industry.

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Section: Multiplex Sensor Developmentmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Amplification-free, highly sensitive electrochemical DNA-based sensor for simultaneous detection of stx1 and stx2 genes of Shiga toxin-producing E. coli (STEC)

O’Riordan

Wasiewska

Diaz

et al. 2022

Preprint

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“…Titanium dioxide is a material of high technological interest due to its photocatalytic, dielectric, and transparent-conductor features that differ between the crystal phases. 63 The ALD of TiO 2 has been studied widely 64 on different surfaces and using various precursors. TiO 2 is one of the few materials for which the ALD has already been studied by operando APXPS.…”

Section: Research Example: Tio 2 Deposited On Native Oxide-covered Simentioning

confidence: 99%

Ambient pressure x-ray photoelectron spectroscopy setup for synchrotron-based in situ and operando atomic layer deposition research

Kokkonen

Kaipio

Nieminen

et al. 2022

Review of Scientific Instruments

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“…Dimensions in the range of several micrometers have been shown to be effective for biosensor applications. [93,94] However, nanometer-scale of digits is still being studied to achieve workability under ambient condition. An electric field between the fingers is produced when a direct current (DC) or alternating current (AC) is applied on the pads.…”

Section: Interdigitated Electrode (Ide)mentioning

confidence: 99%

State-of-the-Art on Functional Titanium Dioxide-Integrated Nano-Hybrids in Electrical Biosensors

Nadzirah

Gopinath

Parmin

et al. 2020

Critical Reviews in Analytical Chemistry

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Biosensors operating based on electrical methods are being accelerated toward rapid and efficient detection that improve the performance of the device. Continuous study in nano-and material-sciences has led to the inflection with properties of nanomaterials that fit the trend parallel to the biosensor evolution. Advancements in technology that focuses on nano-hybrid are being used to develop biosensors with better detection strategies. In this sense, titanium dioxide (TiO 2) nanomaterials have attracted extensive interest in the construction of electrical biosensors. The formation of TiO 2 nano-hybrid as an electrical transducing material has revealed good results with high performance. The modification of the sensing portion with a combination (nano-hybrid form) of nanomaterials has produced excellent sensors in terms of stability, reproducibility, and enhanced sensitivity. This review highlights recent research advancements with functional TiO 2 nano-hybrid materials, and their victorious story in the construction of electrical biosensors are discussed. Future research directions with commercialization of these devices and their extensive utilizations are also discussed.

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Titanium dioxide–mediated resistive nanobiosensor for E. coli O157:H7

Cited by 19 publications

References 39 publications

Amplification-free, highly sensitive electrochemical DNA-based sensor for simultaneous detection of stx1 and stx2 genes of Shiga toxin-producing E. coli (STEC)

Amplification-free, highly sensitive electrochemical DNA-based sensor for simultaneous detection of stx1 and stx2 genes of Shiga toxin-producing E. coli (STEC)

Ambient pressure x-ray photoelectron spectroscopy setup for synchrotron-based in situ and operando atomic layer deposition research

State-of-the-Art on Functional Titanium Dioxide-Integrated Nano-Hybrids in Electrical Biosensors

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