Titanium dioxide nanoparticle-induced testicular damage, spermatogenesis suppression, and gene expression alterations in male mice

Gao, Guodong; Ze, Yuguan; Zhao, Xiaoyang; Sang, Xuezi; Zheng, Lei; Ze, Xiao; Shen, Guanghui; Sheng, Lei; Sun, Qingqing; Hong, Jie; Yu, Xiaohong; Wang, Ling; Hong, Fashui; Zhang, Xueguang

doi:10.1016/j.jhazmat.2013.04.046

Cited by 147 publications

(173 citation statements)

References 59 publications

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“…NAC significantly attenuated these effects as reported in our findings (Farombi et al, 2008). Moreover, high doses of TiO2 was shown to induce deleterious pathological changes, including rare sperm, sperm breakages, rarefaction of Sertoli cell and Sertoli cell apoptosis, necrosis of the seminiferous tubules, decreased germinative layer thickness, vaculation and irregular arrangement of sertoli cells of the seminiferous tubules (Gao et al, 2013).…”

Section: Ajptsupporting

confidence: 84%

Potential Ameliorative Role of N-Acetylcysteine Against Testicular Dysfunction Induced by Titanium Dioxide in Male Albino Rats

El-Kirdasy¹

2014

American Journal of Pharmacology and Toxicology

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In this study, we examined the ameliorative action of N-Acetylcysteine (NAC) against Titanium Dioxide (TiO2) induced testicular degeneration in albino rats. Adult male albino rats were given saline as a control group, TiO2 (1200 mg kg −1 BW), NAC (100 mg kg −1 BW) and co-treatment of NAC and TiO2 as a protective group for 3 months. Testicular tissues were extracted for changes in testicular gene expression and histopathology. Administration of TiO2 significantly up-regulated mRNA expression of IL-6 and TNF-α and normalized by NAC administration. TiO2 administration down regulated Glutathione-S-Transferase (GST) while increased B-cell Lymphoma2 (BcL2) expressions. Co-administration of rats by NAC together with TiO2 normalized changes in GST and BcL2 expression. Expression of steroidogenesis related genes [Androgen Binding Protein (ABR), 17β-Hydroxysteroid Dehydrogenase (17β-HSD), cytochrome P450 17A (CYP17α) and aromatase] showed down regulation in TiO2 administered groups and normalized when NAC given together with TiO2. Moreover, TiO2 induced toxicity in testes that accompanied by degeneration in seminiferous tubules with congestion, oedema and cell disruption that are partially normalized by co-administration of NAC with TiO2. In conclusion, the present findings confirmed the benficial effect of NAC to prevent apoptosis in spermatogenic and sertoli cells and testicular dysfunction induced by TiO2 in male albino rats.

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Section: Ajptsupporting

confidence: 84%

Potential Ameliorative Role of N-Acetylcysteine Against Testicular Dysfunction Induced by Titanium Dioxide in Male Albino Rats

El-Kirdasy¹

2014

American Journal of Pharmacology and Toxicology

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“…As such, evaluation of the LOAEL of TiNPs may need to be conducted cautiously. The liver and testis have been reported as target organs that present a TiNP-induced disorder, which occurs when TiNPs is administered for consecutive days; for example, administered for 90 d (2.5 to 10 mg/kg) (Gao et al, 2013) or administered for 35 days (300 mg/kg) (Orazizadeh et al, 2014). Our published data (Miura et al, 2014) and results in this paper indicated that administration of TiNPs only four times (once a week for 4 weeks; 0.1 to 10 mg/kg) showed a clear testicular dysfunction (Fig.…”

Section: Discussionmentioning

confidence: 99%

High sensitivity of testicular function to titanium nanoparticles

et al. 2017

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-Titanium dioxide nanoparticles (TiNPs) present toxicity in organs such as the liver, lung, and intestine. The testis has also been reported as a target organ of TiNPs. We recently reported that TiNPs had no genotoxic effect in the liver and bone marrow, while showing clear testicular dysfunction. In this paper, therefore, we systematically compared the sensitivity of hepatic function using biochemical markers and testicular function against TiNPs. Male C57BL/6J mice were injected intravenously with TiNPs (Aeroxide-P25, at doses of 0.1, 1, 2, and 10 mg/kg body weight) once per week for 4 consecutive weeks. Mice were sacrificed three days after the last injection. Body weights, liver weights, and testicular-related organ weights were not found to be changed by TiNP treatment. Moreover, TiNPs caused no hepatic damage, as evaluated by alanine aminotransferase and aspartate aminotransferase indexes. The testicular function, however, was clearly impaired by TiNP treatment; reduction in two sperm motion parameters (motile percent and progressive percent) and sperm numbers in cauda epididymides was seen. We observed Ti accumulation in the liver but not in the testis, as well as no change in plasma levels of sex hormones related to spermatogenesis. Our findings indicate that the testis is highly sensitive to TiNPs, as compared to the liver. We believe that, when considering the biological effects of TiNPs, testicular function (especially motility ability) may be a sensitive indicator.

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“…Furthermore, the expression of genes related to spermatogenesis and steroid and hormone metabolism was altered [29].…”

Section: Effects Of Tio 2 Nanoparticle On Male Reproductive Systemmentioning

confidence: 99%

Effect of titanium dioxide nanoparticles on male and female reproductive systems

Alaee¹,

Ilani²

2017

Journal of Advanced Medical Sciences and Applied Technologies

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Titanium Dioxide (TiO 2 ) nanoparticle has a wide range of application in industrial and consumer products especially in cosmetics such as high sun protection factor creams in order to protect the skin from UV light. In spite of its increased production and use there is not enough epidemiological data regarding TiO 2 nanoparticle toxicity. Toxic effects of TiO 2 nanoparticles on human reproductive systems have been investigated by many studies mostly by employing animal models, but results are extremely conflicted and inconsistent. In this review we summarized published data about the effects of TiO 2 nanoparticle on male and female reproductive systems to clarify its possible toxic effects on reproduction and fertility.

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Titanium dioxide nanoparticle-induced testicular damage, spermatogenesis suppression, and gene expression alterations in male mice

Cited by 147 publications

References 59 publications

Potential Ameliorative Role of N-Acetylcysteine Against Testicular Dysfunction Induced by Titanium Dioxide in Male Albino Rats

Potential Ameliorative Role of N-Acetylcysteine Against Testicular Dysfunction Induced by Titanium Dioxide in Male Albino Rats

High sensitivity of testicular function to titanium nanoparticles

Effect of titanium dioxide nanoparticles on male and female reproductive systems

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