Titanium-doped cerium oxide nanoparticles protect cells from hydrogen peroxide-induced apoptosis

Abstract

To develop new nanoparticle materials possessing anti-oxidative capacity with improved physical characteristics, we have studied titanium-doped cerium oxide (CeTiO2) nanoparticles. CeTiO2 nanoparticles had a mode diameter of 15-20 nm. These nanoparticles demonstrated catalase activity, and did not promote the activation of hemolytic or cytolytic pathways in living cells. Using surface plasmon resonance enhanced microscopy, we find that these nanoparticles associate with cells. Transmission electron microscopy studies demonstrated that these nanoparticles accumulate within the vacuolar compartment of cells. Importantly, CeTiO2 nanoparticles decrease hydrogen peroxide-mediated apoptosis of cells as judged by the reduced cleavage of a caspase 3-sensitive label. CeTiO2 nanoparticles may contribute to deflecting tissue damage in a broad spectrum of oxidant-mediated diseases, such as macular degeneration and Alzheimer's disease.

Role of Nao-ceria in the amelioration of oxidative stress: Current and future applications in medicine

“…Anti-oxidative by scavenging the free radicals in the retina Pourkhalili et al, 2011 Decrease progression of diabetes reduce the level of oxidative damage by scavenging the free oxygen species due to diabetes Zhou et al, 2011 Alzheimer's disease titanium-doped 15-20 nm Contribute to deflecting tissue damage in a broad spectrum of oxidant-mediated diseases, such as macular degeneration and Alzheimer's disease. Clark et al, 2013 Potential application of the nano-ceria in the medicine…”

Intracellular Delivery of Antioxidant CeO2 Nanoparticles via Polyelectrolyte Microcapsules

“…This was done using a model of hydrogen peroxide induced oxidative stress. As shown previously, this model can be used to provide an adequate assessment of the therapeutic potential of cerium oxide nanoparticles. , We used higher concentrations of hydrogen peroxide (1.5 mM for 30 min), in comparison with previous reports, , to demonstrate high catalase-like activity of encapsulated nanoceria.…”

Surface Chemistry of Biologically Active Reducible Oxide Nanozymes

“…[ 164 ] This study demonstrated no change in the CAT‐like activity of CeTiO 2 NPs following incubation in culture medium. [ 165 ] As such, this area of rNZ–corona research warrants further investigation. Under healthy physiological conditions, cells, and proteins maintain a reduced state, and it is conceivable that the presence of oxidizing rNZs may disrupt this balance by depleting electrons from aqueous redox proteins and/or redox couples, or by acting as catalysts.…”