Titanium nanotopography induces osteocyte lacunar-canalicular networks to strengthen osseointegration

He, Xindie; Yamada, Masahiro; Watanabe, Jobu; Tiskratok, Watcharaphol; Ishibashi, Minoru; Kitaura, Hideki; Mizoguchi, Itaru; Egusa, Hiroshi

doi:10.1016/j.actbio.2022.08.023

Cited by 18 publications

(5 citation statements)

References 50 publications

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“…As depicted in Figure 3 , the surface of pure titanium sheet is relatively smooth and dense, with the surface elements predominantly composed of titanium. After alkali activation treatment, the content of oxygen increased to 43.31%, the surface has a honeycomb grid structure and there are a lot of cracks, which was consistent with the phenomenon observed by He et al ( Kokubo and Yamaguchi, 2015 ; He et al, 2022 ; Jiang et al, 2023 ). After loading a layer of PLL, the bottom and surface of the fissure groove seem to be covered with fog, which is a very small PLL deposition.…”

Section: Resultssupporting

confidence: 90%

Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants

You,

Wang,

Wang

et al. 2024

Front. Bioeng. Biotechnol.

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The construction of an antibacterial biological coating on titanium surface plays an important role in the long-term stability of oral implant restoration. Graphene oxide (GO) has been widely studied because of its excellent antibacterial properties and osteogenic activity. However, striking a balance between its biological toxicity and antibacterial properties remains a significant challenge with GO. ε-poly-L-lysine (PLL) has broad-spectrum antibacterial activity and ultra-high safety performance. Using Layer-by-layer self-assembly technology (LBL), different layers of PLL/GO coatings and GO self-assembly coatings were assembled on the surface of titanium sheet. The materials were characterized using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and contact angle test. The antibacterial properties of Porphyromonas gingivalis (P.g.) were analyzed through SEM, coated plate experiment, and inhibition zone experiment. CCK-8 was used to determine the cytotoxicity of the material to MC3T3 cells, and zebrafish larvae and embryos were used to determine the developmental toxicity and inflammatory effects of the material. The results show that the combined assembly of 20 layers of GO and PLL exhibits good antibacterial properties and no biological toxicity, suggesting a potential application for a titanium-based implant modification scheme.

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Section: Resultssupporting

confidence: 90%

Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants

You,

Wang,

Wang

et al. 2024

Front. Bioeng. Biotechnol.

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“…Other viewpoints hold that rats have the same growth factors, cytokines, and chemokines that regulate the bone remodeling process in humans and that these similarities justify using rats in osseointegration research [30,31]. For instance, recent studies used rats to evaluate the effect of smoke exposure on the bone metabolism around implants coated with nano-hydroxyapatite [32] and the influence of nano-roughened surfaces on the osseointegration strength [33] (Figure 1). Also, this animal model was used to evaluate the collagen fiber formation around an implant during the osseointegration process [34] and compare the histological levels of osseointegration of titanium vs. zirconia dental implants [35].…”

Section: Use Of Animal Models In Pre-clinical Researchmentioning

confidence: 99%

Animal Models for Investigating Osseointegration: An Overview of Implant Research over the Last Three Decades

Scarano,

Khater,

Gehrke

et al. 2024

JFB

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Dental implants and bone augmentation are among dentistry’s most prevalent surgical treatments; hence, many dental implant surfaces and bone grafts have been researched to improve bone response. Such new materials were radiologically, histologically, and histomorphometrically evaluated on animals before being used on humans. As a result, several studies used animals to evaluate novel implant technologies, biocompatibility, surgical techniques, and osseointegration strategies, as preclinical research on animal models is essential to evaluate bioactive principles (on cells, compounds, and implants) that can act through multiple mechanisms and to predict animal behavior, which is difficult to predict from in vitro studies alone. In this study, we critically reviewed all research on different animal models investigating the osseointegration degree of new implant surfaces, reporting different species used in the osseointegration research over the last 30 years. Moreover, this is the first study to summarize reviews on the main animal models used in the translational research of osseointegration, including the advantages and limitations of each model and determining the ideal location for investigating osseointegration in small and large animal models. Overall, each model has advantages and disadvantages; hence, animal selection should be based on the cost of acquisition, animal care, acceptability to society, availability, tolerance to captivity, and housing convenience. Among small animal models, rabbits are an ideal model for biological observations around implants, and it is worth noting that osseointegration was discovered in the rabbit model and successfully applied to humans.

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“…Furthermore, highly interconnected osteocytes are also reported adjacent to nanopatterned implant surfaces characterised by ~ 75 nm wide hemispherical features produced by colloidal lithography [ 30 ]. Nanopatterning achieved by alkaline-etching also has a demonstrable positive impact on osteocyte dendricity compared to acid-etched, micro-rough implants in a rat model [ 74 ].…”

Section: Osteocytes In Peri-implant Bonementioning

confidence: 99%

Contributions of Resin Cast Etching to Visualising the Osteocyte Lacuno-Canalicular Network Architecture in Bone Biology and Tissue Engineering

Sato

Shah

2023

Calcif Tissue Int

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Recent years have witnessed an evolution of imaging technologies towards sophisticated approaches for visualising cells within their natural environment(s) and for investigating their interactions with other cells, with adjacent anatomical structures, and with implanted biomaterials. Resin cast etching (RCE) is an uncomplicated technique involving sequential acid etching and alkali digestion of resin embedded bone to observe the osteocyte lacuno-canalicular network using scanning electron microscopy. This review summarises the applicability of RCE to bone and the bone-implant interface. Quantitative parameters such as osteocyte size, osteocyte density, and number of canaliculi per osteocyte, and qualitative metrics including osteocyte shape, disturbances in the arrangement of osteocytes and canaliculi, and physical communication between osteocytes and implant surfaces can be investigated. Ageing, osteoporosis, long-term immobilisation, spinal cord injury, osteoarthritis, irradiation, and chronic kidney disease have been shown to impact osteocyte lacuno-canalicular network morphology. In addition to titanium, calcium phosphates, and bioactive glass, observation of direct connectivity between osteocytes and cobalt chromium provides new insights into the osseointegration potential of materials conventionally viewed as non-osseointegrating. Other applications include in vivo and in vitro testing of polymer-based tissue engineering scaffolds and tissue-engineered ossicles, validation of ectopic osteochondral defect models, ex vivo organ culture of whole bones, and observing the effects of gene dysfunction/deletion on the osteocyte lacuno-canalicular network. Without additional contrast staining, any resin embedded specimen (including clinical biopsies) can be used for RCE. The multitude of applications described here attest to the versatility of RCE for routine use within correlative analytical workflows, particularly in biomaterials science.

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Titanium nanotopography induces osteocyte lacunar-canalicular networks to strengthen osseointegration

Cited by 18 publications

References 50 publications

Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants

Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants

Animal Models for Investigating Osseointegration: An Overview of Implant Research over the Last Three Decades

Contributions of Resin Cast Etching to Visualising the Osteocyte Lacuno-Canalicular Network Architecture in Bone Biology and Tissue Engineering

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