Title: Potassium application regulates nitrogen metabolism and osmotic adjustment in cotton ( Gossypium hirsutum L.) functional leaf under drought stress

Zahoor, Rizwan; Zhao, Wenqing; Abid, Muhammad; Dong, Haoran; Zhou, Zhiguo

doi:10.1016/j.jplph.2017.05.001

Cited by 143 publications

(91 citation statements)

References 40 publications

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“…(Konishi & Yanagisawa, 2011). Reduction in NR activity in water deficit conditions was also observed in other studies (Meng et al, 2015;Zahoor, Zhao, Abid, Dong, & Zhou, 2017).…”

Section: Leaf Gas Exchangesupporting

confidence: 81%

Physiological Responses of Physalis angulata Plants to Water Deficit

Leite¹,

Nascimento²,

Tanan³

et al. 2018

JAS

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It’s known that drought affects crop growth, however, little is known about the physiological responses developed under these conditions by underexploited species, such as Physalis angulata. This study aimed at assessing the physiological responses of Physalis angulata plants after 40 days under different water availability (100%, 80%, 60%, 40% and 20% of pot field capacity). In this research, the effects of the water deficit on the relative water content, water potential, gas exchange, sugars accumulation and activity of nitrate reductase were evaluated. Water relations were affected mainly in plants under severe water deficit, however, the variables remained stable when cultivated at sub-optimal levels of field capacity. Gas exchanges were also affected by water deficit, with reduction in carbon assimilation, internal carbon, stomatal conductance and transpiration, as well as increased leaf temperature and water use efficiency. Plants accumulated sugars as a mechanism of tolerance to severe water deficit, while nitrate reductase activity was reduced. P. angulata plants develop important strategies to tolerate water deficit, contributing to the establishment of crops under low water availability.

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“…(Konishi & Yanagisawa, 2011). Reduction in NR activity in water deficit conditions was also observed in other studies (Meng et al, 2015;Zahoor, Zhao, Abid, Dong, & Zhou, 2017).…”

Section: Leaf Gas Exchangesupporting

confidence: 81%

Physiological Responses of Physalis angulata Plants to Water Deficit

Leite¹,

Nascimento²,

Tanan³

et al. 2018

JAS

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“…Previous studies showed that drought stress regulated N metabolism balance through changes in N metabolic enzyme activities [17,18,32]. Drought stress decreased activities of NR and GOGAT enzymes in both leaves and roots of Populus simonii seedlings [67] and activities of NR, GS, and GOGAT enzymes in cotton leaves [32]. The changes of enzyme activities can directly affect the efficiency of N uptake and utilization.…”

Section: Discussionmentioning

confidence: 97%

“…The mixture was incubated at 25 • C for 20 min to change color and then centrifuged at 15,000× g for 10 min. The absorbance of supernatant was measured at A540 nm according to Zahoor et al [32]. GS activity was calculated from the standard curve of c-glutamyl hydroxamate.…”

Section: N-metabolizing Enzymes Extraction and Analysismentioning

confidence: 99%

“…Furthermore, proline and some other nitrogen compounds are important osmotic adjustment (OA) substances involved in various metabolic pathways in response to abiotic stress [29][30][31], including maintaining cell turgor and subcellular structures, quenching free radicals, and buffering cellular redox potential [29]. Accumulation and synthesis of compatible solutes (amino acids and soluble sugars) and ions (NO 3 − ) are considered as main contributors to OA in plants under drought stress [29,32]. Moreover, previous studies have shown that nitrogen assimilation products, including NO 3 − , NH 4 + , and proline, can regulate the selective absorption and transport of ions in plants and play an important role in maintaining the dynamic balance of physiological metabolism in plants under abiotic stress [33][34][35].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Drought Stress at Reproductive Stages on Growth and Nitrogen Metabolism in Soybean

Zhao

Chen

et al. 2020

Agronomy

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This study aims to determine variability among soybean (Glycine max (L.) Merr.) cultivars under drought conditions and how nitrogen metabolites, metabolism-related enzymes, and gene expression vary during soybean growth. Three soybean cultivars, Shennong17 (CV.SN17), Shennong8 (CV.SN8), and Shennong12 (CV.SN12), were grown in pot culture and subjected to drought stress at reproductive stages for 45 days. The results showed that long-term drought stress decreased biomass allocation to reproductive organs, weakened antioxidant capacity, and reduced seed weight, effects that were less pronounced in CV.SN12 compared with those in CV.SN8 and CV.SN17. Drought stress decreased the concentrations of nitrogen and soluble protein but increased nitrate concentration in leaves. This was related to the significantly reduction of nitrogen metabolism efficiency, including decreased activities of nitrogen metabolism enzymes, and downregulated expression of GmNR, GmNiR, GmGS, and GmGOGAT. Drought stress increased the concentrations of free amino acid, proline, and soluble sugar in leaves to enhance the osmotic adjustment ability. Furthermore, soybean seed weight showed significantly correlation (p < 0.05) with nitrogen-metabolism-related parameters. Based on the performance of growth, nitrogen metabolism, and yield attributes, CV.SN12 showed the highest tolerance to drought, followed by CV.SN8 and CV.SN17. In addition, these nitrogen-metabolism-related parameters could be used in soybeans to select for drought tolerance.

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“…Decreased soluble protein contents have also been observed in drought-stressed plant species such as barley [59], cotton [60], and maize [61]. Zhang et al [52] reported that the protein degradation of tomato seedlings exposed to saline-alkaline stress could be inhibited by exogenous spermidine by relieving disturbances in N metabolism.…”

Section: Discussionmentioning

confidence: 99%

Exogenous DCPTA Increases the Tolerance of Maize Seedlings to PEG-Simulated Drought by Regulating Nitrogen Metabolism-Related Enzymes

Xie

et al. 2019

Agronomy

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2-(3,4-Dichlorophenoxy) triethylamine (DCPTA) regulates plant development; however, the molecular basis of this regulation is poorly understood. In this study, RNA sequencing (RNA-seq) analysis and physiological indexes of maize seedlings (three-leaf stage) treated with 15% polyethylene glycol (PEG) with/without DCPTA were investigated to explore the possible mechanism of exogenous DCPTA-improved drought tolerance. In the library pair comparisons of DCPTA vs. the control, PEG vs. the control, and PEG + DCPTA vs. PEG, totals of 19, 38 and 20 differentially expressed genes (DEGs) were classified as being involved in metabolic processes, respectively; totals of 5, 11, and 6 DEGs were enriched in the nitrogen (N) metabolic pathway, respectively. The genes encoding nicotinamide adenine dinucleotide-nitrate reductase (NADH-NR), ferredoxin-nitrite reductase (Fd-NiR), reduced ferredoxin-glutamate synthase (Fd-GOGAT), and chloroplastic glutamine synthetase (GS 2) were common in response to PEG-simulated drought stress with/without DCPTA treatment. Moreover, DCPTA maintained stable gene relative expression levels and protein abundances of NADH-NR, Fd-NiR, GS2, and Fd-GOGAT. Moreover, exogenous DCPTA partially mitigated PEG-simulated drought-induced reductions in the enzymatic activities of NR, nitrite reductase (NiR), glutamine synthase (GS), glutamine oxoglutarate aminotransferase (GOGAT), and transaminase, as well as in the contents of nitrate (NO 3 − ), nitrite (NO 2 − ) and soluble proteins and increases in the contents of ammonium (NH 4 + ) and free amino acids. Together, our results indicate that exogenous DCPTA improved plant growth and drought tolerance by regulating N-mechanism enzymatic activities involved in transcription and enzymatic protein synthesis.

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Title: Potassium application regulates nitrogen metabolism and osmotic adjustment in cotton ( Gossypium hirsutum L.) functional leaf under drought stress

Cited by 143 publications

References 40 publications

Physiological Responses of Physalis angulata Plants to Water Deficit

Physiological Responses of Physalis angulata Plants to Water Deficit

Effect of Drought Stress at Reproductive Stages on Growth and Nitrogen Metabolism in Soybean

Exogenous DCPTA Increases the Tolerance of Maize Seedlings to PEG-Simulated Drought by Regulating Nitrogen Metabolism-Related Enzymes

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