Titration and Partial Characterization of a Soluble Hemolysin of a Group D Streptococcus

Irwin, John; Seeley, H. W.

doi:10.1128/jb.76.1.29-35.1958

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…Earlier studies on a group D hemolysin were performed by Irwin and Seeley (1958) but, since the strain they used is no longer available (Seeley, personal communication), we cannot compare our results with theirs.…”

Section: Omentioning

confidence: 89%

Probable Identity of a Group D Hemolysin With a Bacteriocine

1963

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Bloomington) AND JOSEPH M. DAVIE. Probable identity of a group D hemolysin with a bacteriocine. J. Bacteriol. 86:708-712. 1963.-All strains of Streptococcus zymogenes (S. faecalis var. zymogenes) produce a bacteriocine which is active against lactic acid bacteria and most other grampositive bacteria. Mutants which have lost the hemolytic characteristic lose at the same time their bacteriocine-producing ability. A strain which was resistant to the bacteriocine but which was nonhemolytic and nonbacteriocinogenic was irradiated, and two hemolytic mutants were isolated from it. These mutants were also bacteriocinogenic. Thus, the two activities are gained or lost together by mutation. Both activities are destroyed by chloroform vapors and are antagonized by lecithin. Both activities are destroyed at the same rate by treatment at 45 C under mildly acid conditions, and both activities are stable when heated in agar. The two activities are produced in parallel during the growth cycle, and disappear in parallel. The possible ecological role of a substance which is both a hemolysin and a bacteriocine is discussed.

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Section: Omentioning

confidence: 89%

Probable Identity of a Group D Hemolysin With a Bacteriocine

1963

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“…All reports concerning hemolysin production by the group D streptococci have dealt with preparations obtained from cells grown in complex media. For example, a serum-beef infusion broth was used by Plummer (18), whereas Irwin and Seeley (17) found that a polynucleotide from yeast autolysate could stimulate the activity of hemolysin when this polynucleotide was used to supplement their neopeptone-beef infusion medium. The studies reported by Brock and his co-workers and Jackson's group have also dealt with hemolysin produced in other complex media: Todd-Hewitt and brain heart infusion (BHI) broths, respectively.…”

mentioning

confidence: 99%

Production of Hemolysin and Bacteriolysin in a Synthetic Medium by Streptococcus faecalis var. zymogenes

Appelbaum

Zimmerman

1974

Infect Immun

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A synthetic medium has been developed in which Streptococcus faecalis var. zymogenes X-14 elaborates a lysin. The medium consists of 18 amino acids, lactose, six vitamins, adenine, guanine, uracil, and six salts. Glucose, and K,HPO, in excess of 0.5% (wt/vol), were inhibitory to lysin production; increasing concentrations of L-arginine-hydrochloride gave increasing yields of lytic activity. Brock et al. (4), in a survey of the bacteriocins of the enterococci, described a bacteriocin from ' Authorized for publication by The Experiment Station, Pennsylvania State University, as paper no. 4560.

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