1999
DOI: 10.1038/sj.onc.1202762
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Titration of cellular export factors, but not heteromultimerization, is the molecular mechanism of trans-dominant HTLV-1 Rex mutants

Abstract: The HTLV-1 Rex protein is an essential shuttle protein required for nuclear export of unspliced and incompletely-spliced viral RNAs. Several trans-dominant (TD) mutant Rex proteins have been reported, however, the mechanism of trans-dominance is not known. We compared TD Rex mutants and found that a natural occurring Rex mutant, Rexp21, lacking the RNA binding domain, was highly TD and inhibited also HIV-1 Rev function. Using fusions to the green uorescent protein (GFP) we observed that Rexp21-GFP displayed a … Show more

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Cited by 34 publications
(37 citation statements)
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“…4C). Our results contradict a previous paper suggesting that p21Rex may inhibit p27Rex (24). In that study, the authors used a 4-to 10-fold excess of a p21RexGFP expression vector in order to see an effect on p27Rex-mediated export.…”
Section: Resultscontrasting
confidence: 56%
“…4C). Our results contradict a previous paper suggesting that p21Rex may inhibit p27Rex (24). In that study, the authors used a 4-to 10-fold excess of a p21RexGFP expression vector in order to see an effect on p27Rex-mediated export.…”
Section: Resultscontrasting
confidence: 56%
“…3A). The low level of fluorescence of RemGFP was also absent from the nucleoli, which is typical of GFPRem and other Rev-like proteins (21,37,43) (see Fig. 3B) and reminiscent of the behavior of Rem cleavage site mutants (7).…”
Section: Discussionmentioning
confidence: 95%
“…The same localization and tra cking pattern was observed in the p53 negative cell line H1299 (e,f) or the p53 and Mdm2 negative cell line X174 (g,h). The presence of p21Rex-BFP in the same cell was veri®ed using the appropriate ®lter to detect BFP and mediated by the CRM1 export pathway (Fornerod et al, 1997;Heger et al, 1999;Stauber et al, 1998a). To understand how adenoviral or retroviral shuttle proteins exploit this export pathway, we reasoned that a shuttle protein with a high a nity for common export factors should be able to a ect the tra cking of a shuttle protein with a lower a nity in trans.…”
Section: Retroviral Shuttle Proteins Are Not Affected By E1b Traffickmentioning
confidence: 99%