Tjp3/zo-3 is critical for epidermal barrier function in zebrafish embryos

Kiener, Tanja K.; Selptsova-Friedrich, Inna; Hunziker, Walter

doi:10.1016/j.ydbio.2007.12.047

Cited by 59 publications

(50 citation statements)

References 34 publications

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“…Similar developmental defects were also observed in zebrafish larvae experiencing ZO-3 knockdown (22). ZO-3 is a scaffolding protein that interacts with transmembrane tight junction proteins and cytoskeleton actin, and is suggested to play a role in maintaining epithelial integrity (22). Using a fluorescent paracellular marker FD-4, we observed that the accumulation of FD-4 was confined mainly to the yolk sac and pericardial cavity in the claudin-b morphants.…”

Section: Knockdown Of Claudin-b Causes Developmental Defects and Incrsupporting

confidence: 69%

“…Therefore, it is possible that the tail malformation in the claudin-b morphants was associated with Na ϩ imbalance (discussed below) during early stages of development. Similar developmental defects were also observed in zebrafish larvae experiencing ZO-3 knockdown (22). ZO-3 is a scaffolding protein that interacts with transmembrane tight junction proteins and cytoskeleton actin, and is suggested to play a role in maintaining epithelial integrity (22).…”

Section: Knockdown Of Claudin-b Causes Developmental Defects and Incrmentioning

confidence: 58%

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The tight junction protein claudin-b regulates epithelial permeability and sodium handling in larval zebrafish,Danio rerio

Kwong

Perry

2013

American Journal of Physiology-Regulatory, Integrative and Comp

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Kwong RWM, Perry SF. The tight junction protein claudin-b regulates epithelial permeability and sodium handling in larval zebrafish, Danio rerio. Am J Physiol Regul Integr Comp Physiol 304: R504 -R513, 2013. First published January 13, 2013 doi:10.1152/ajpregu.00385.2012.-The functional role of the tight junction protein claudin-b in larval zebrafish (Danio rerio) was investigated. We showed that claudin-b protein is expressed at epithelial cell-cell contacts on the skin. Translational gene knockdown of claudin-b protein expression caused developmental defects, including edema in the pericardial cavity and yolk sac. Claudin-b morphants exhibited an increase in epithelial permeability to the paracellular marker polyethylene glycol (PEG-4000) and fluorescein isothiocyanate-dextran (FD-4). Accumulation of FD-4 was confined mainly to the yolk sac and pericardial cavity in the claudin-b morphants, suggesting these regions became particularly leaky in the absence of claudin-b expression. Additionally, Na ϩ efflux was substantially increased in the claudin-b morphants, which contributed to a significant reduction in whole-body Na ϩ levels. These results indicate that claudin-b normally acts as a paracellular barrier to Na ϩ . Nevertheless, the elevated loss of Na ϩ in the morphants was compensated by an increase in Na ϩ uptake. Notably, we observed that the increased Na ϩ uptake in the morphants was attenuated in the presence of the selective Na ϩ /Cl Ϫ -cotransporter (NCC) inhibitor metolazone, or during exposure to Cl Ϫ -free water. These results suggested that the increased Na ϩ uptake in the morphants was, at least in part, mediated by NCC. Furthermore, treatment with an H ϩ -ATPase inhibitor bafilomycin A1 was found to reduce Na ϩ uptake in the morphants, suggesting that H ϩ -ATPase activity was essential to provide a driving force for Na ϩ uptake. Overall, the results suggest that claudin-b plays an important role in regulating epithelial permeability and Na ϩ handling in zebrafish.claudin-b; epithelial permeability; sodium; tight junction proteins; zebrafish TIGHT JUNCTIONS (TJS) ARE important components regulating the paracellular movement of solutes and water in vertebrates. TJs are composed of four major transmembrane proteins: occludin, claudins, tricellulins, and junctional adhesion molecule. However, claudins are thought to be the major determinant of the epithelial barrier functions (35). To date, more than 20 claudin isoforms have been identified in mammals (16), as well as in teleost fish (9,24,27,32), and the expression of these claudin isoforms appears to be cell-and tissue-specific. Studies using mammalian cell models have shown that the various claudin isoforms exhibit marked differences in their permeability characteristics, presumably establishing unique properties for different epithelia and endothelia (34,37). It has also been demonstrated that some claudin isoforms can form ion-specific seals or pores to regulate the paracellular movement of ions (1,20). The ion selectivity of claudins is sug...

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Section: Knockdown Of Claudin-b Causes Developmental Defects and Incrsupporting

confidence: 69%

Section: Knockdown Of Claudin-b Causes Developmental Defects and Incrmentioning

confidence: 58%

The tight junction protein claudin-b regulates epithelial permeability and sodium handling in larval zebrafish,Danio rerio

Kwong

Perry

2013

American Journal of Physiology-Regulatory, Integrative and Comp

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“…18 ZO proteins play an important role in maintaining barrier integrity formed by epithelial cells, and loss of ZO-3 in particular results in increased permeability of cell layers. 19 Recently, Sharma et al 15 showed glucose causes translocation of ZO-1 from the cell membrane to the cytoplasm in cultured podocytes. We observed similarly increased cytoplasmic and decreased membrane ZO-3 staining in renal tubules in human diabetic kidneys.…”

Section: Discussionmentioning

confidence: 99%

Urinary Peptidome May Predict Renal Function Decline in Type 1 Diabetes and Microalbuminuria

Merchant

Perkins

Boratyn

et al. 2009

Journal of the American Society of Nephrology

138

112

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One third of patients with type 1 diabetes and microalbuminuria experience an early, progressive decline in renal function that leads to advanced stages of chronic kidney disease and ESRD. We hypothesized that the urinary proteome may distinguish between stable renal function and early renal function decline among patients with type 1 diabetes and microalbuminuria. We followed patients with normal renal function and microalbuminuria for 10 to 12 yr and classified them into case patients (n ϭ 21) with progressive early renal function decline and control subjects (n ϭ 40) with stable renal function. Using liquid chromatography matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, we identified three peptides that decreased in the urine of patients with early renal function decline [fragments of ␣1(IV) and ␣1(V) collagens and tenascin-X] and three peptides that increased (fragments of inositol pentakisphosphate 2-kinase, zona occludens 3, and FAT tumor suppressor 2). In renal biopsies from patients with early nephropathy from type 1 diabetes, we observed increased expression of inositol pentakisphosphate 2-kinase, which was present in granule-like cytoplasmic structures, and zona occludens 3. These results indicate that urinary peptide fragments reflect changes in expression of intact protein in the kidney, suggesting new potential mediators of diabetic nephropathy and candidate biomarkers for progressive renal function decline. 20: 206520: -207420: , 200920: . doi: 10.1681 Microalbuminuria (MA) has been the primary diagnostic test to identify patients who have type 1 diabetes and are at risk for overt proteinuria and subsequent declining renal function leading to ESRD 1,2 ; however, the predictive value of MA is now questioned. First, a large proportion of patients with MA can revert to normoalbuminuria 3-5 ; second, only a minority of patients with MA progress to proteinuria 3,6 ; and, third, in one third of patients with MA, progressive renal function decline starts already at the onset of MA, not proteinuria. The last process we refer to as early renal function decline (ERFD). 7 Our understanding of the disease process underlying ERFD is limited. The existing hypotheses about the cause of diabetic nephropathy (DN) are in question given that progressive ERFD is initiated J Am Soc Nephrol

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“…However, in a recent study, the function of the TJ scaffold protein tight junction protein-3/zonula occludens-3 (Tjp-3/Zo-3) was investigated using antisense morpholino oligonucleotides (MOs; Kiener et al, 2008). Despite the fact that Tjp-3/Zo-3 is expressed throughout cleavage, blastula and gastrula stages, no early defects were observed in morpholino injected embryos.…”

Section: Introductionmentioning

confidence: 99%

“…Despite the fact that Tjp-3/Zo-3 is expressed throughout cleavage, blastula and gastrula stages, no early defects were observed in morpholino injected embryos. At later stages, morphant embryos displayed tail fin defects, pericardial edema, and a lack of blood circulation (Kiener et al, 2008). These phenotypes were ascribed to defects in the EVL permeability barrier, resulting in defective osmoregulation.…”

Section: Introductionmentioning

confidence: 99%

The tight junction component Claudin E is required for zebrafish epiboly

Siddiqui

Sheikh

Tran

et al. 2010

Developmental Dynamics

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Zebrafish epiboly results in the thinning and spreading of the blastoderm to cover the yolk cell and close the blastopore. The extra-embryonic yolk syncytial layer (YSL) tows the blastoderm vegetally during epiboly by means of its tight junction attachments to the enveloping layer (EVL). Claudins are the major transmembrane protein components of tight junctions. Here, we focus on the function of Claudin E (Cldne), which is expressed specifically in the EVL. Morpholino knock-down of cldne produced a highly penetrant epiboly delay. Our analysis suggested that the EVL margin, which is attached to the YSL, was under reduced tension in morphant embryos. We propose that local variation in the strength of EVL-YSL attachment in morphant embryos resulted in slow and uneven advancement of the EVL and blastoderm. Our work is the first to demonstrate that Claudins are important for zebrafish epiboly. Developmental Dynamics 239:715-722,

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Tjp3/zo-3 is critical for epidermal barrier function in zebrafish embryos

Cited by 59 publications

References 34 publications

The tight junction protein claudin-b regulates epithelial permeability and sodium handling in larval zebrafish,Danio rerio

The tight junction protein claudin-b regulates epithelial permeability and sodium handling in larval zebrafish,Danio rerio

Urinary Peptidome May Predict Renal Function Decline in Type 1 Diabetes and Microalbuminuria

The tight junction component Claudin E is required for zebrafish epiboly

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