TLR4-Mediated Sensing of <i>Campylobacter jejuni</i> by Dendritic Cells Is Determined by Sialylation

Kuijf, Mark L.; Samsom, Janneke N.; Rijs, Wouter van; Bax, Marieke; Huizinga, Ruth; Heikema, Astrid P.; Doorn, Pieter A. van; Belkum, Alex van; Kooyk, Yvette van; Burgers, Peter C.; Luider, Theo M.; Endtz, Hubert P.; Nieuwenhuis, Edward E. S.; Jacobs, Bart C.

doi:10.4049/jimmunol.0903014

Cited by 75 publications

(86 citation statements)

References 40 publications

(61 reference statements)

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“…Using nonsialylated LOS derived from cst-II-deficient GB11 C. jejuni, a similar kinetics was observed (Fig. 1B), only the extent of the response was markedly reduced, as reported previously (14).…”

Section: Jejuni Los-stimulated Dcs Rapidly Produce Factors That Ensupporting

confidence: 89%

“…GB19 was isolated from a patient with Miller Fisher syndrome and has a GD1c-mimicking LOS (20). LOS were isolated and purified according to the hot-phenol extraction method (14). LOS from GB11 wild-type (wt), but not from GB11 cst-II, were recognized by cholera toxin, confirming the presence of GM1 mimicry.…”

Section: Bacteria and Los Preparationsmentioning

confidence: 92%

“…2). As a reference, we correlated B cell proliferation with IL-6, because we previously showed that this cytokine was not involved in DC-mediated B cell proliferation (14). Of these cytokines, TNF-a and IFN-b showed the highest correlation coefficients (r 2 = +0.70 and +0.67, respectively), whereas IL-10 and IL-6 correlated poorly (r 2 = +0.30 and +0.20, respectively).…”

Section: Ifn-b and Tnf-a From Los-stimulated Dcs Mediate B Cell Prolimentioning

confidence: 99%

“…LOS are rough types of endotoxin, because they lack the polysaccharide O-chain, and activate TLR4 (12,13). Notably, sialylation of C. jejuni LOS increases the activation of human dendritic cells (DCs) and mouse myeloid cells (14,15). How sialic acid influences the endotoxicity of C. jejuni LOS is not known, but it is possible that its presence alters the engagement with the TLR4-MD-2 complex or with upstream molecules, such as CD14 or LPS-binding protein (LBP), which act as cofactors in the recognition of endotoxins (16).…”

mentioning

confidence: 99%

“…Because myeloid cells, such as DCs, are important for bridging innate and adaptive immunity, as well as play an important role in the development of humoral immunity to T-independent Ags (17)(18)(19), we previously investigated the potential of DCs stimulated with C. jejuni LOS to promote B cell responses. It was shown that human monocyte-derived DCs stimulated with C. jejuni LOS produce soluble factors that enhance B cell proliferation, independently of T cells (14).…”

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confidence: 99%

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Sialylation of Campylobacter jejuni Endotoxin Promotes Dendritic Cell–Mediated B Cell Responses through CD14-Dependent Production of IFN-β and TNF-α

Huizinga

Rijs

Bajramović

et al. 2013

The Journal of Immunology

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Campylobacter jejuni is the most common bacterial cause of human gastroenteritis and often precedes development of Guillain–Barré syndrome (GBS), a life-threatening paralytic disease. The incorporation of the carbohydrate sialic acid into C. jejuni lipooligosaccharides (LOS) is associated with increased severity of gastroenteritis and with induction of GBS; however, the underlying mechanisms remain completely unknown. In this study, we demonstrate that sialic acids in C. jejuni endotoxin enhance the rapid production of IFN-β and TNF-α by human dendritic cells (DCs). Using neutralizing Abs and receptors it was shown that these DC-derived cytokines promote the proliferation of human mucosal B cells in a T cell–independent manner. The production of both IFN-β and TNF-α by DCs in response to LOS requires CD14, and the amplified response of DCs to sialylated C. jejuni LOS is CD14 dependent. Together, these results indicate that sialylation of C. jejuni LOS increases DC activation and promotes subsequent B cell responses through CD14-driven production of IFN-β and TNF-α. This enhanced DC/B cell response may explain the increased pathogenicity of sialylated C. jejuni and may be key to the initiation of B cell–mediated autoimmunity in GBS.

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Section: Jejuni Los-stimulated Dcs Rapidly Produce Factors That Ensupporting

confidence: 89%

Section: Bacteria and Los Preparationsmentioning

confidence: 92%

Section: Ifn-b and Tnf-a From Los-stimulated Dcs Mediate B Cell Prolimentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Sialylation of Campylobacter jejuni Endotoxin Promotes Dendritic Cell–Mediated B Cell Responses through CD14-Dependent Production of IFN-β and TNF-α

Huizinga

Rijs

Bajramović

et al. 2013

The Journal of Immunology

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Antecedent infections in Guillain‐Barré syndrome: a single‐center, prospective study

Hao

Wang

Jacobs³

et al. 2019

Ann Clin Transl Neurol

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ObjectiveTo investigate the spectrum of antecedent infections in Chinese patients with Guillain‐Barré syndrome (GBS) and analyze the infections‐related clinical phenotypes locally.MethodsA prospective case‐control study of 150 patients diagnosed with GBS and age‐ and sex‐matched neurological and healthy controls was performed to investigate recent infections of 14 pathogens serologically and collect the clinical data during a follow‐up of 12 months.ResultsIn total, 53% of patients with GBS had a positive serology for recent infection, including Campylobacter jejuni (27%), influenza A (17%) and B (16%), hepatitis A virus (5%), dengue virus (3%), cytomegalovirus (3%), Epstein–Barr virus (3%), Mycoplasma pneumoniae (2%), herpes simplex virus (2%), varicella‐zoster virus (1%), and rubella virus (1%). Serology for infections of hepatitis E virus, Haemophilus influenzae, and Zika virus was negative. There was a higher frequency of C. jejuni, influenza A, influenza B, and hepatitis A virus infections in GBS patients than both the neurological and healthy controls. C. jejuni infection was more frequent in younger GBS patients and was associated with antibodies against GM1, GalNAc‐GD1a, and GM1:galactocerebroside complex. Influenza B infection was associated with a pure motor form of GBS.Interpretation C. jejuni, influenza A, influenza B, and hepatitis A virus serve as the most common cause of antecedent infections in GBS locally. Influenza B‐related GBS may represent a pure motor phenotype. Differences in the infectious spectrum worldwide may contribute to the geographical clinical heterogeneity of GBS.

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Innate Immunity to Campylobacter jejuni in Guillain‐Barré Syndrome

Huizinga

Berg

Rijs

et al. 2015

Annals of Neurology

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TLR4-Mediated Sensing of Campylobacter jejuni by Dendritic Cells Is Determined by Sialylation

Abstract: Material

Cited by 75 publications

References 40 publications

Sialylation of Campylobacter jejuni Endotoxin Promotes Dendritic Cell–Mediated B Cell Responses through CD14-Dependent Production of IFN-β and TNF-α

Sialylation of Campylobacter jejuni Endotoxin Promotes Dendritic Cell–Mediated B Cell Responses through CD14-Dependent Production of IFN-β and TNF-α

Antecedent infections in Guillain‐Barré syndrome: a single‐center, prospective study

Innate Immunity to Campylobacter jejuni in Guillain‐Barré Syndrome

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