“…To assess TLR recognition of E. bovis-derived antigens (EbAg), HEK cells expressing bovine TLR2 or bovine TLR4 containing an NF-κB luciferase reporter (NF-κB-luc, Promega, Chilworth, Hampshire, UK) were used [56,72,73]. Briefly, HEK-TLR2 or HEK-TLR4 cells were seeded at a density of 2.5 × 10 5 cells in 6-well plates using Dulbecco's Modified Eagle Medium (DMEM Life Technologies, Paisley, Renfrewshire, UK) supplemented with 10% fetal bovine serum (FBS, Life Technologies, Paisley, Renfrewshire, UK) and 1 mg mL −1 Geneticin (Life Technologies, Paisley, Renfrewshire, UK).…”