TLR9 Negatively Regulates Intracellular Bacterial Killing by Pyroptosis in Burkholderia pseudomallei
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            Infected Mouse Macrophage Cell Line (Raw264.7)

Pudla, Matsayapan; Sanongkiet, Sucharat; Ekchariyawat, Peeraya; Luangjindarat, Chularat; Ponpuak, Marisa; Utaisincharoen, Pongsak

doi:10.1128/spectrum.03488-22

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“…Cell death has a crucial role in aspects of immune responses against pathogen infection. Pyroptosis, a type of regulated cell death, controls microbial survival and contributes to the regulation of the host immune response (37)(38)(39). Recent studies of the role of pyroptosis in Kp infection have suggested that Kp-induced pyroptosis is important for the development of host immunity.…”

Section: Discussionmentioning

confidence: 99%

Resistance of hypervirulent Klebsiella pneumoniae to cathepsin B-mediated pyroptosis in murine macrophages

et al. 2023

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IntroductionHypervirulent Klebsiella pneumoniae (hvKp) has emerged as a clinically significant global pathogen in the last decade. However, the host immune responses of the macrophages during hvKp infection are largely unknown. In the present study, we aimed to compare the cytotoxic effects of hvKp and classical K. pneumoniae (cKp) in murine macrophages.ResultsWe found that the activation of caspase-1 -dependent pyroptosis was higher in cKp-infected macrophages compared with that in hvKp-infected macrophages. In Caspase-1 deficiency macrophages, pyroptosis diminished during infection. Both hvKp and cKp strains led to nucleotide-binding and oligomerization domain-like receptor protein 3 (NLRP3) inflammasome formation and lysosomal cathepsin B activation, thus resulting in pyroptosis. Compared with the cKp strain, the hvKp strain inhibited these phenomena in murine macrophages.ConclusionHvKp infection resulted in different levels of pyroptosis via the activation of cathepsin B-NLRP3-caspase-1 in murine macrophages. Therefore, the manipulation of pyroptotic cell death is a potential target for host response during hvKp infection in macrophages.

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Section: Discussionmentioning

confidence: 99%

Resistance of hypervirulent Klebsiella pneumoniae to cathepsin B-mediated pyroptosis in murine macrophages

et al. 2023

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TLR9 Negatively Regulates Intracellular Bacterial Killing by Pyroptosis in Burkholderia pseudomallei - Infected Mouse Macrophage Cell Line (Raw264.7)

Abstract: Surface TLRs have been well established to play an essential role in Burkholderia pseudomallei infection. However, the role of endosomal TLRs has not been elucidated.

Cited by 1 publication

References 45 publications

Resistance of hypervirulent Klebsiella pneumoniae to cathepsin B-mediated pyroptosis in murine macrophages

Resistance of hypervirulent Klebsiella pneumoniae to cathepsin B-mediated pyroptosis in murine macrophages

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