tmRNA abundance inStreptomyces aureofaciens, S. griseus andS. collinus under stress-inducing conditions

Palečková, Petra; Felsberg, Jürgen; Bobek, Jan; Mikulı́k, Karel

doi:10.1007/bf02932105

Cited by 9 publications

(5 citation statements)

References 31 publications

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“…205,209 By controlling these genes, tmRNA plays an important role in stress control, and indeed mutants in ssrA (encoding tmRNA) are highly sensitive to exposure to antibiotics. [209][210][211] As the tmRNA system is required for production of DasR, it possibly has a role in regulating its cellular abundance and, in turn, antibiotic production.…”

Section: Antagonism Of Dasr By Atra and Rok7b7mentioning

confidence: 99%

The regulation of the secondary metabolism of Streptomyces: new links and experimental advances

2011

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Streptomycetes and other actinobacteria are renowned as a rich source of natural products of clinical, agricultural and biotechnological value. They are being mined with renewed vigour, supported by genome sequencing efforts, which have revealed a coding capacity for secondary metabolites in vast excess of expectations that were based on the detection of antibiotic activities under standard laboratory conditions. Here we review what is known about the control of production of so-called secondary metabolites in streptomycetes, with an emphasis on examples where details of the underlying regulatory mechanisms are known. Intriguing links between nutritional regulators, primary and secondary metabolism and morphological development are discussed, and new data are included on the carbon control of development and antibiotic production, and on aspects of the regulation of the biosynthesis of microbial hormones. Given the tide of antibiotic resistance emerging in pathogens, this review is peppered with approaches that may expand the screening of streptomycetes for new antibiotics by awakening expression of cryptic antibiotic biosynthetic genes. New technologies are also described that have potential to greatly further our understanding of gene regulation in what is an area fertile for discovery and exploitation

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Section: Antagonism Of Dasr By Atra and Rok7b7mentioning

confidence: 99%

The regulation of the secondary metabolism of Streptomyces: new links and experimental advances

2011

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“…Consistently, the total amount of trans -translation products increases under stressful conditions (Fujihara et al, 2002). Perhaps in response to the increased requirement of the trans -translation system, the intracellular level of tmRNA or SmpB increases with an increase in stress in some bacteria (Muto et al, 2000; Palecková et al, 2007; Rezzonico et al, 2007). …”

Section: Introductionmentioning

confidence: 99%

tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell

2014

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Transfer messenger RNA (tmRNA; also known as 10Sa RNA or SsrA RNA) is a small RNA molecule that is conserved among bacteria. It has structural and functional similarities to tRNA: it has an upper half of the tRNA-like structure, its 5’ end is processed by RNase P, it has typical tRNA-specific base modifications, it is aminoacylated with alanine, it binds to EF-Tu after aminoacylation and it enters the ribosome with EF-Tu and GTP. However, tmRNA lacks an anticodon, and instead it has a coding sequence for a short peptide called tag-peptide. An elaborate interplay of actions of tmRNA as both tRNA and mRNA with the help of a tmRNA-binding protein, SmpB, facilitates trans-translation, which produces a single polypeptide from two mRNA molecules. Initially alanyl-tmRNA in complex with EF-Tu and SmpB enters the vacant A-site of the stalled ribosome like aminoacyl-tRNA but without a codon–anticodon interaction, and subsequently truncated mRNA is replaced with the tag-encoding region of tmRNA. During these processes, not only tmRNA but also SmpB structurally and functionally mimics both tRNA and mRNA. Thus trans-translation rescues the stalled ribosome, thereby allowing recycling of the ribosome. Since the tag-peptide serves as a target of AAA+ proteases, the trans-translation products are preferentially degraded so that they do not accumulate in the cell. Although alternative rescue systems have recently been revealed, trans-translation is the only system that universally exists in bacteria. Furthermore, it is unique in that it employs a small RNA and that it prevents accumulation of non-functional proteins from truncated mRNA in the cell. It might play the major role in rescuing the stalled translation in the bacterial cell.

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“…Confirmation of the expected employment of small RNAs in the regulation of cell processes, including primary metabolism, developmental transitions, antibiotic production, and various stress responses is being increasingly reported (Palecková et al, 2007 ; Pánek et al, 2008 ; Swiercz et al, 2008 ; D'Alia et al, 2010 ; Mikulík et al, 2014 ). Hundreds of cis- acting asRNAs were identified using RNA sequencing in two recent studies (Vockenhuber et al, 2011 ; Moody et al, 2013 ).…”

Section: Introductionmentioning

confidence: 92%

RNase III-Binding-mRNAs Revealed Novel Complementary Transcripts in Streptomyces

Šetinová

Šmídová

Pohl³

et al. 2018

Front. Microbiol.

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cis-Antisense RNAs (asRNAs) provide very simple and effective gene expression control due to the perfect complementarity between regulated and regulatory transcripts. In Streptomyces, the antibiotic-producing clade, the antisense control system is not yet understood, although it might direct the organism's complex development. Initial studies in Streptomyces have found a number of asRNAs. Apart from this, hundreds of mRNAs have been shown to bind RNase III, the double strand-specific endoribonuclease. In this study, we tested 17 mRNAs that have been previously co-precipitated with RNase III for antisense expression. Our RACE mapping showed that all of these mRNAs possess cognate asRNA. Additional tests for antisense expression uncovered as-adpA, as-rnc, as3983, as-sigB, as-sigH, and as-sigR RNAs. Northern blots detected the expression profiles of 18 novel transcripts. Noteworthy, we also found that only a minority of asRNAs respond to the absence of RNase III enzyme by increasing their cellular levels. Our findings suggest that antisense expression is widespread in Streptomyces, including genes of such important developmental regulators, as AdpA, RNase III, and sigma factors.

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tmRNA abundance inStreptomyces aureofaciens, S. griseus andS. collinus under stress-inducing conditions

Cited by 9 publications

References 31 publications

The regulation of the secondary metabolism of Streptomyces: new links and experimental advances

The regulation of the secondary metabolism of Streptomyces: new links and experimental advances

tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell

RNase III-Binding-mRNAs Revealed Novel Complementary Transcripts in Streptomyces

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