TMT‐based proteomics analysis of the effects of Qianjinweijing Tang on lung cancer

Abstract

Qianjinweijing Tang (QJWJ) is a classic traditional Chinese formula that is often used in the treatment of treat lung cancer (LC). However, the underlying cellular mechanisms of the anticancer effects of QJWJ remain unclear. Cell viability was determined by MTS assay and levels of apoptosis measured by flow cytometry.Animal experiments were conducted to determine the effects of QJWJ on tumor growth in vivo. We used a proteomics approach to study the effects of QJWJ on LC cells and applied bioinformatics analysis to identify differentially expressed proteins that were validated by western blotting. QJWJ inhibited the proliferation of LC cells and induced apoptosis. The tumor growth delay effects of QJWJ were confirmed in vivo. We identified 104 differentially expressed proteins following QJWJ treatments of which 45 proteins were upregulated and 59 were downregulated. The levels of differentially expressed proteins were validated by western blotting. Our study indicated that QJWJ has anticancer effects in vivo and in vitro and that these effects are mediated by modulating the expression of tumor-related proteins.

TMT Quantitative Proteomics Analysis Reveals the Effects of Transport Stress on Iron Metabolism in the Liver of Chicken

“…At the individual level, the liver maintains iron homeostasis by balancing iron supply with iron utilization and losses. The liver orchestrates systemic iron balance by producing and secreting hepcidin, inducing degradation of the iron exporter ferroportin, which regulates the import of iron from the bloodstream to the liver [ 17 ]. The liver has been shown to be a hub for iron regulation, but the effect TS on hepatic iron metabolism is unknown.…”

Exploring the Anti-Inflammatory Effect of Tryptanthrin by Regulating TLR4/MyD88/ROS/NF-κB, JAK/STAT3, and Keap1/Nrf2 Signaling Pathways

“…The samples were divided into control group and LPS and TRYP (2.5 μg/mL) groups. The methods of protein extraction and peptide enzymatic digestion were described in the previous literature . Briefly, the lysate was added to extract the protein and quantified.…”

“…The methods of protein extraction and peptide enzymatic digestion were described in the previous literature. 58 Briefly, the lysate was added to extract the protein and quantified. After that, the proteins were trypsinized, desalted, and lyophilized.…”