Tn2008-driven carbapenem resistance in Acinetobacter baumannii isolates from a period of increased incidence of infections in a Southwest Virginia hospital (USA)

Rao, Jayasimha; Susanti, Dwi; Childress, Johnathon C.; Mitkos, M.C.; Brima, Joshua K.; Baffoe-Bonnie, Anthony; Pearce, Samuel N.; Grgurich, Dale; Fernandez-Cotarelo, Maria Jose; Kerkering, Thomas M.; Mukhopadhyay, Biswarup

doi:10.1016/j.jgar.2017.08.017

Cited by 11 publications

(10 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, it was found that the aphA6 in majority of the isolates is located in Tn aphA6 . This finding is consistent with the previous studies reporting that the aphA6 gene is frequently located in Tn aphA6 [8–10].…”

Section: Discussionsupporting

confidence: 94%

“…To date, Tn aphA6 -carrying plasmids belonging to repAci6 plasmid family have appeared to be mainly restricted to the Australian isolates and have only occasionally been found in the limited number of isolates from Italy [11], Romania [24], and the United States [10]. The aphA6 has been detected not only in A. baumannii but also on a plasmid of Escherichia coli and Klebsiella pneumoniae isolates from Republic of the Union of Myanmar, Australia, and Italy [25–27].…”

Section: Discussionmentioning

confidence: 99%

“…It is demonstrated that ISAba125 plays a role in overexpression of the aphA6 and high-level resistance to amikacin, kanamycin, and neomycin [6, 7]. Tn aphA6 was first detected in the Australian isolates belonging to global clone 2 (GC2), then in a member of global clone 1 (GC1) from Australia [8, 9], and lately in the isolates containing pCMCVT Ab 2-type plasmids from the United States [10]. Tn aphA6 was also found on pACICU2 in the same location as pAb-G7–2 [11].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Amikacin resistance due to the aphA6 gene in multi-antibiotic resistant Acinetobacter baumannii isolates belonging to global clone 1 from Iran

2019

View full text Add to dashboard Cite

Background TnaphA6-carrying repAci6 plasmids have been detected in Acinetobacter baumannii isolates belonging to global clones, GC1 and GC2, worldwide. Here, we examined whether RepAci6 plasmids family play a role in the dissemination of the aphA6 in GC1 A. baumannii isolates from Iran. Results We found that 22 isolates carried the repAci6 gene, suggesting that they contain a RepAci6 plasmid family. Using the primers linking the aphA6 gene to the backbone of repAci6 plasmid, it was revealed that 16 isolates from different hospitals harbored TnaphA6 on a repAci6 plasmid. Conclusions This study provides evidence for the dissemination of TnaphA6 on the plasmids encoding RepAci6 in Iranian A. baumannii isolates. Furthermore, it seems that TnaphA6 might be acquired by distinct plasmids separately as it was found to be located on the variants of repAci6 plasmids.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Amikacin resistance due to the aphA6 gene in multi-antibiotic resistant Acinetobacter baumannii isolates belonging to global clone 1 from Iran

2019

View full text Add to dashboard Cite

show abstract

“…A single colony of CMC-115 was grown for 18 h in 25 ml lysogeny broth at 37°C, at 200 rpm. The cell pellet was used for genomic DNA isolation with a Genomic-tip 20/G (Qiagen) (5). The genome was sequenced on the Illumina NextSeq platform at the Virginia Tech Genomics Resource Center, with a library constructed using the Illumina TruSeq DNA preparation kit.…”

mentioning

confidence: 99%

Complete Genome Sequence of Pseudomonas aeruginosa CMC-115, a Clinical Strain from an Acute Ventilator-Associated Pneumonia Patient

Adenikinju

Jensen

Kerkering

et al. 2020

Microbiol Resour Announc

Self Cite

View full text Add to dashboard Cite

show abstract

“…PCR was carried out using primers for pcrV gene and rahU gene used as comparative product by using genomic DNA (gDNA) as a template, as described earlier [42]. The PCR was set up as follows: 23 μl of platinum Taq PCR Supermix (Invitrogen) including 0.5 μl of forward and reverse primers for pcrV and rahU together, then 2 μl of DNA template from each strain was added to give a final volume of 25 μl.…”

Section: Polymerase Chain Reaction (Pcr) For Pcrv Genementioning

confidence: 99%

Comparative Transcriptomic Analysis of <em>Pseudomonas aeruginosa</em> Isolates from ICU Patients with Acute and Chronic Pneumonia

Jayasimha¹,

Adenikinju²,

Matthew³

et al. 2021

Int Arch Med Microbiol

View full text Add to dashboard Cite

Multidrug-resistant (MDR) and virulent pathogenic strains of Pseudomonas aeruginosa (Pa) are among the most dangerous pathogens in healthcare-associated infections. In this study, a comparative analysis was performed on two clinical Pa strains from pneumonia patients, 1) An acute strain (CMC-115) and 2) A chronic multidrug-and carbapenemresistant strain (CMC-097) with distinct phenotypic and genetic characteristics. A transcriptome analysis using both the Pseudomonas DNA GeneChip microarray and Illumina RNA-Seq technologies was carried out for both strains at early-stationary growth phase in laboratory culture. The comparative transcriptomic analysis identified 134 genes differentially expressed ≥ 4.0-fold by both technologies. Between the two strains, which included virulence genes such as flagellar, pili, pyoverdine, and phenazine genes, that were higher in the acute strain, CMC-115, and type 3 secretory system (T3SS) and pyochelin biosynthesis genes, that were higher in the chronic strain, CMC-097. In particular, the DNA microarrays suggested and the RNA-Seq analysis confirms the type 3 secretory system genes were completely missing from the acute strain, CMC-115.The combined analysis using the RNA-Seq and DNA microarray methods also identified important genes that were either missing or highly divergent from the Reference PAO1 genome used for the DNA microarray design. For example, the RNA-Seq data identified an operon containing severalmultidrug resistance genes: aacA27, β-lactamase OXA-2 (bla OXA-2 ), qacΔE, sul1, and GNAT-N, in the chronic strain, CMC-097. This comparative analysis revealed several important differences in gene expression between the two strains that may lead to identification of targets for better therapy and hospital management.

show abstract

Tn2008-driven carbapenem resistance in Acinetobacter baumannii isolates from a period of increased incidence of infections in a Southwest Virginia hospital (USA)

Cited by 11 publications

References 38 publications

Amikacin resistance due to the aphA6 gene in multi-antibiotic resistant Acinetobacter baumannii isolates belonging to global clone 1 from Iran

Amikacin resistance due to the aphA6 gene in multi-antibiotic resistant Acinetobacter baumannii isolates belonging to global clone 1 from Iran

Complete Genome Sequence of Pseudomonas aeruginosa CMC-115, a Clinical Strain from an Acute Ventilator-Associated Pneumonia Patient

Comparative Transcriptomic Analysis of <em>Pseudomonas aeruginosa</em> Isolates from ICU Patients with Acute and Chronic Pneumonia

Contact Info

Product

Resources

About