TNF-α and endotoxin increase hypoxia-induced VEGF production by cultured human nasal fibroblasts in synergistic fashion

Sun, Dong; Matsune, Shoji; Ohori, Junichiro; Fukuiwa, Tatsuya; Ushikai, Masato; Kurono, Yuichi

doi:10.1016/j.anl.2005.01.004

Cited by 29 publications

(26 citation statements)

References 29 publications

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“…VEGF is an angiogenic growth factor and it plays a vital role in the neovascularization of the middle ear mucosa during OM in mice and also an important role in inflammatory events together with other inflammatory cytokines [20,21]. Increasing expression of VEGF protein in middle ear of OME model induced by endotoxin and hypoxia indicated that VEGF might be associated with the pathogenesis of OME [22,23]. Studies have reported that both VEGF receptor-1 and VEGF receptor-2 are up-regulated during experimental OM in the rat [24].…”

Section: Discussionmentioning

confidence: 99%

Blocking macrophage migration inhibitory factor activity alleviates mouse acute otitis media in vivo

Zhang

Zheng

et al. 2014

Immunology Letters

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This study was to investigate the role of macrophage migration inhibitory factor (MIF) in mouse acute otitis media (AOM), we hypothesize that blocking MIF activity will relieve mouse AOM. A mouse AOM model was constructed by injecting lipopolysaccharide (LPS) into the middle ear of C57BL/6 mice through the tympanic membrane (TM). MIF levels were measured by real-time PCR (RT-PCR) and ELISA after LPS application. Normal or AOM mice were given PBS or ISO-1 (MIF antagonist) every day for 10 days and the hearing levels were determined by measuring auditory brainstem response (ABR) threshold. After the ABR test finished, H&E staining was conducted and the inflammation was also measured by detecting interleukin (IL)-1β, tumor necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF) levels with RT-PCR and ELISA. TLR-4 expression was determined by western blotting and NF-κB activation was determined by electrophoretic mobility shift assays. Compared with the normal control, MIF levels in the middle ear of LPS-induced AOM mice were significant increased. The ABR results showed that mean ABR thresholds in ISO-1 treated AOM mice were significantly reduced compared with PBS treated AOM mice since day 7, indicating that ISO-1 treatment potentially improved the hearing levels of AOM mice. H&Estaining showed that ISO-1 treatment could reduce the mucosal thickness of AOM mice. In ISO-1 treated mice, TLR-4 expression and levels of IL-1β, TNF-α and VEGF were significantly lower compared with PBS treated AOM mice. ISO-1 treatment also significantly inhibited NF-κB activation in AOM mice compared with PBS treated AOM mice. These results suggested that blocking the activity of MIF by ISO-1 could reduce the inflammation in AOM mice in which process TLR-4 and NF-κB were involved. The reduction in MIF activity is conducive to alleviate mouse AOM, which may serve as a potential therapeutic target for the treatment of AOM.

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Section: Discussionmentioning

confidence: 99%

Blocking macrophage migration inhibitory factor activity alleviates mouse acute otitis media in vivo

Zhang

Zheng

et al. 2014

Immunology Letters

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“…VEGF is an important angiogenic cytokine with critical roles in tumor angiogenesis [7] and associated with the invasion and metastasis of malignant tumors, promotes angiogenesis and enhances vascular permeability [37]. VEGF, the multifunctional cytokine plays a crucial role in mediating neovascularization and has been taken as a marker Table 5 Plasma T-NOS values and positive numbers of VEGF staining of rats in different groups.…”

Section: Discussionmentioning

confidence: 99%

Effect of selenium-enriched malt on VEGF and several relevant angiogenic cytokines in diethylnitrosamine-induced hepatocarcinoma rats

Liu

Zhao

Liu

et al. 2010

Journal of Trace Elements in Medicine and Biology

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“…Primer sequences were VEGF subtype forward, 5Ј-GTCTATCAGCGCAGCTACTG-3Ј; VEGF subtype reverse, 5Ј-CCGCCTCGGCTTGTCACA-3Ј; ␤-actin sense, 5Ј-GTGGGGCGCCCCAGGCACCA-3Ј; ␤-actin antisense, 5Ј-CTCCTTAATGTCACGCACGATTTC-3Ј. 13,14 …”

Section: Reverse Transcriptase-polymerase Chain Reactionmentioning

confidence: 99%

Vascular Endothelial Growth Factor Produced in Nasal Glands of Perennial Allergic Rhinitis

Matsune

Ohori

Sun

et al. 2008

American Journal of Rhinology

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TNF-α and endotoxin increase hypoxia-induced VEGF production by cultured human nasal fibroblasts in synergistic fashion

Cited by 29 publications

References 29 publications

Blocking macrophage migration inhibitory factor activity alleviates mouse acute otitis media in vivo

Blocking macrophage migration inhibitory factor activity alleviates mouse acute otitis media in vivo

Effect of selenium-enriched malt on VEGF and several relevant angiogenic cytokines in diethylnitrosamine-induced hepatocarcinoma rats

Vascular Endothelial Growth Factor Produced in Nasal Glands of Perennial Allergic Rhinitis

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